Specialized Equipment

Bioreader 3000 Eli-Spot Reader

Elispot assays are a relatively simple means of assessing the cytokine-secreting function of antigen-specific lymphocytes. Lymphocytes are incubated in 96 well plates along with antigen, and cytokines released by activated cells are "captured" by cytokine-specific antibodies, appearing as coloured spots on the well surface. The Bioreader 3000 is a high throughput, high resolution Elispot reader capable of reproducibly counting up to 2,000 spots per well.

Contact:
Dr. Jacqueline Lai
cheukyan@mail.ubc.ca | 604-875-2245

Cellomics

The ArrayScan VTI is a high content screening (HCS) instrument capable of simultaneously imaging up to six cellular functions in a single cell using fluorescence imaging. Examples of how this instrument has been used at the Research Institute include assessments of cell growth, apoptosis, invasion, nuclear translocation, differentiation, library screening (small interfering RNAs and chemicals), neuronal imaging and identification of rare subcellular populations i.e. cancer stem cells are readily detectable and quantified.

Contact coming soon

Kodak X-Omat 2000A Film Processor

The Kodak X-Omat 2000A film processor is a tabletop processor designed for both low and medium volume processing applications. The processor combines proven reliability and image quality with a selection of two processing cycles. The rapid cycle (90 seconds) processes 125 34 x 43 cm films an hour and is recommended for Kodak T-Mat films and similar general-purpose radiography films. The standard cycle (120 seconds) processes 100 34 x 43 films an hour.

Contact:
Nick Steel
nsteel@bcchr.ca | 604-875-2000 ext. 7975

Microscopy

Several microscope facilities are available on site.

Metabolic Assessment and Indirect Calorimetry

The laboratory for obesity genetics and indirect calorimetry is a research facility that allows detailed, high-throughput energy balance measurements in mouse models of human disease. The laboratory allows for measurement of food and liquid intake, oxygen consumption, carbon dioxide production and locomotor activity (infrared beam-breaking). All measurements are performed continuously in the home cage environment, using the LabMaster system (TSE-Systems, Germany). Body composition is measured noninvasively using Quantitative Magnetic Resonance (QMR - Echo Medical Systems, Texas). These experiments are ideal for detailed, muticomponent phenotyping of mice for obesity susceptibility, fasting/refeeding and the metabolic response to dietary changes.

Contacts:
Dr. William Gibson
wgibson@cw.bc.ca

Katelin Townsend
604-875-2000 ext. 6783.

Radioisotope Facility

Contact:
Roger Dyer
radyer@mail.ubc.ca | 604-875-2418