Overview

CALIPER: A Canada-wide Initiative to Close the Critical Gaps in Pediatric Reference Intervals

The diagnosis and care of pediatric patients is critically dependent on laboratory test results. Physicians assess test results and consider diagnoses by comparing these results to ‘normal ranges’. The validity of these normal ranges may vary depending on the the age and gender of subjects used to derive them. Additionally, different test methods exist that produce numerically different results, such that results and normal ranges are not interchangeable. Normal ranges for pediatrics for many laboratory tests are inaccurate and outdated. Also, for those tests that have good pediatric normal ranges available, there has been difficulty and delay in laboratories to report them.

CALIPER is a Canada-wide initiative to develop, implement, and maintain accurate and up-to-date normal ranges for any laboratory tests performed on children. CALIPER collects and analyzes small blood samples from healthy Canadian children of all ethnicities so that the results benefit our diverse population. Seven pediatric hospitals from across Canada, including BC Children's Hospital, are collaborating on the CALIPER project. We are currently planning to expand efforts to study reference ranges in neonates.

Publications

Limitations and opportunities of whole blood bilirubin measurements by GEM premier 4000®
BMC Pediatrics
Li Wang and Arianne Y. K. Albert and Benjamin Jung and Keyvan Hadad and Martha E. Lyon and Melanie Basso
DOI: 10.1186/s12887-017-0842-8
03/2017

Utility of Ara h 2 sIgE levels to predict peanut allergy in Canadian children
The Journal of Allergy and Clinical Immunology: In Practice
Sara H. Leo and John M. Dean and Benjamin Jung and Boris Kuzeljevic and Edmond S. Chan
DOI: 10.1016/j.jaip.2015.04.001
11/2015

Case report: Hemolytic interference of a kinetic Jaffe creatinine method
Clinical Biochemistry
Dailin Li and Andre Mattman and Benjamin Jung and Adelina Lim
DOI: 10.1016/j.clinbiochem.2015.07.082
10/2015

Negative heterophile antibody interference affecting iPTH
Clinical Biochemistry
Andre Mattman and Benjamin Jung and Kristin Hauff and Laura Burns and Daniel Holmes and Supna Sandhu and Breay Paty
DOI: 10.1016/j.clinbiochem.2015.07.084
10/2015

Comparison of the Nova StatStrip(R) Lactate versus the Arkray Lactate Pro(R) meters for fetal scalp lactate measurement
Clinical Biochemistry
Melanie Basso and Benjamin Jung and Li Wang and Elvira Kozak and Ivy Fernando and Catherine Halstead and Ellen Giesbrecht
DOI: 10.1016/j.clinbiochem.2014.06.036
08/2014

Acute encephalopathy in a kidney transplant recipient following infusion of intravenous immunoglobulin
Transplant International
Li Wang and Nicholas Larkins and Benjamin Jung and Nicholas H.C. Au and Cherry Mammen
DOI: 10.1111/tri.12384
08/2014

Acute encephalopathy due to true hyponatremia and hyperammonemia after fast infusion of intravenous immunoglobulin
Clinical Biochemistry
L. Wang and N. Larkins and B. Jung and N. Au and C. Mammen
DOI: 10.1016/j.clinbiochem.2014.04.077
06/2014

CLSI-based transference of the CALIPER database of pediatric reference intervals from Abbott to Beckman, Ortho, Roche and Siemens Clinical Chemistry Assays: Direct validation using reference samples from the CALIPER cohort
Clinical Biochemistry
Mathew P. Estey and Ashley H. Cohen and David A. Colantonio and Man Khun Chan and Tina Binesh Marvasti and Edward Randell and Edgard Delvin and Jocelyne Cousineau and Vijaylaxmi Grey and Donald Greenway and Qing H. Meng and Benjamin Jung and Jalaluddin Bhuiyan and David Seccombe and Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2013.04.001
09/2013

Discovery of undocumented drug interferences on the Vitros urine methadone immunoassay
Clinical Biochemistry
M. Seyed Ibrahim and R. Saeedi and W. Martz and J. Bhuiyan and B. Jung and A.C. Halstead
DOI: 10.1016/j.clinbiochem.2012.07.056
09/2012

Validation of the Randox total bile acids assay on the Vitros 5600
Clinical Biochemistry
R. Saeedi and M. Seyed Ibrahim and L. Trowski and B. Jung and J. Bhuiyan and A.C. Halstead
DOI: 10.1016/j.clinbiochem.2012.07.055
09/2012

Evaluation of the Sebia isoelectric focusing method for alpha 1 antripsin phenotype determination
Clinical Biochemistry
T.N. Higgins and M. Limcango and B. Jung
DOI: 10.1016/j.clinbiochem.2012.07.036
09/2012

Sanger sequencing solved a cryptic case of severe alpha1-antitrypsin deficiency
Clinical Biochemistry
Shing H. Zhan and Raja T. Abboud and Benjamin Jung and Brian Kuchinka and Diana Ralston and Brett Casey and Andre Mattman
DOI: 10.1016/j.clinbiochem.2012.01.027
04/2012

Alpha1-antitrypsin deficiency: a clinical-genetic overview
The Application of Clinical Genetics
Raja Abboud and Nelson and Tanya and Benjamin Jung and Andre Mattman
DOI: 10.2147/tacg.s10604
03/2011

Analytical evaluation of the VITROS® 5600 Integrated System in a pediatric setting and determination of pediatric reference intervals
Clinical Biochemistry
Ivan M. Blasutig and Benjamin Jung and Vathany Kulasingam and Sanaz Baradaran and Yu Chen and Man Khun Chan and David Colantonio and Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2010.05.011
09/2010

Pediatric reference intervals for 28 chemistries and immunoassays on the Roche cobas® 6000 analyzer—A CALIPER pilot study
Clinical Biochemistry
Vathany Kulasingam and Benjamin P. Jung and Ivan M. Blasutig and Sanaz Baradaran and Man Khun Chan and Mujdat Aytekin and David A. Colantonio and Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2010.05.008
09/2010

Pediatric reference intervals for 28 biochemistry analytes on the Roche cobas® 6000 analyzer
Clinical Biochemistry
V. Kulasingam and B.P. Jung and I.M. Blasutig and S. Baradaran and M.K. Chan and M. Aytekin and D.A. Colantonio and K. Adeli
DOI: 10.1016/j.clinbiochem.2010.04.007
06/2010

Clinical laboratory reference intervals in pediatrics: The CALIPER initiative
Clinical Biochemistry
Benjamin Jung and Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2009.06.025
11/2009

Identification of a murine methyl cytosine phosphate guanine binding domain-containing factor 3 (MBD3) promoter segment sufficient for driving reporter gene expression in neurons in vitro and in vivo
Neuroscience
B.P. Jung and D. Purushotham and J.H. Eubanks
DOI: 10.1016/j.neuroscience.2006.12.057
03/2007

Isolation and characterization of LCHN: a novel factor induced by transient global ischemia in the adult rat hippocampus
Journal of Neurochemistry
Guangming Zhang and Benjamin P. Jung and Warren Ho and Denis G. M. Jugloff and Herman H. Cheung and James W. Gurd and M. Christopher Wallace and James H. Eubanks
DOI: 10.1111/j.1471-4159.2006.04374.x
11/2006

Increased dendritic complexity and axonal length in cultured mouse cortical neurons overexpressing methyl-CpG-binding protein MeCP2
Neurobiology of Disease
Denis G.M. Jugloff and Benjamin P. Jung and Diana Purushotham and Richard Logan and James H. Eubanks
DOI: 10.1016/j.nbd.2004.11.002
06/2005

Decreased expression and impaired function of muscarinic acetylcholine receptors in the rat hippocampus following transient forebrain ischemia
Neurobiology of Disease
Guangming Zhang and Liang Zhang and Richard Logan and Benjamin P. Jung and Darren Yuen and Jee Ching Hsu and James H. Eubanks
DOI: 10.1016/j.nbd.2005.05.011
2005

Differential expression of methyl CpG-binding domain containing factor MBD3 in the developing and adult rat brain
Journal of Neurobiology
Benjamin P. Jung and Guangming Zhang and Romy Nitsch and Judy Trogadis and Sukriti Nag and James H. Eubanks
DOI: 10.1002/neu.10199
05/2003

The expression of methyl CpG binding factor MeCP2 correlates with cellular differentiation in the developing rat brain and in cultured cells
Journal of Neurobiology
Benjamin P. Jung and Denis G.M. Jugloff and Guangming Zhang and Richard Logan and Stephanie Brown and James H. Eubanks
DOI: 10.1002/neu.10201
02/2003

Expression and function of A1 adenosine receptors in the rat hippocampus following transient forebrain ischemia
Neuroscience
H Shen and L Zhang and D Yuen and R Logan and B.P Jung and G Zhang and J.H Eubanks
DOI: 10.1016/s0306-4522(02)00352-4
10/2002

Kindling induces the mRNA expression of methyl DNA-binding factors in the adult rat hippocampus
Neuroscience
J Francis and B Jung and G Zhang and J Cheng and W Ho and W.M Burnham and J.H Eubanks
DOI: 10.1016/s0306-4522(02)00150-1
08/2002

Transient forebrain ischemia alters the mRNA expression of methyl DNA-binding factors in the adult rat hippocampus
Neuroscience
B.P Jung and G Zhang and W Ho and J Francis and J.H Eubanks
DOI: 10.1016/s0306-4522(02)00383-4
2002

Perforant pathway kindling transiently induces the mRNA expression of GABA-B receptor subtypes R1A and R2 in the adult rat hippocampus
Molecular Brain Research
Joseph Francis and Benjamin P. Jung and Guangming Zhang and Warren Ho and Jerome Cheng and W. McIntyre Burnham and James H. Eubanks
DOI: 10.1016/s0169-328x(01)00122-x
07/2001

Somatostatin type 2 receptor expression in the rat hippocampus following cerebral ischemia
Neuroreport
Warren Ho and Benjamin P. Jung and Guangming Zhang and James H. Eubanks
DOI: 10.1097/00001756-200107200-00013
07/2001

Differential increase in Fos immunoreactivity in hypothalamic and septal nuclei by arginine8-vasopressin and desglycinamide9-arginine8-vasopressin
Neuroscience
A.J Lança and P.H Wu and B Jung and J.-F Liu and V Ng and H Kalant
DOI: 10.1016/s0306-4522(98)00709-x
07/1999

Cloning and chromosomal mapping of four putative novel human G-protein-coupled receptor genes
Gene
Brian F O'Dowd and Tuan Nguyen and Benjamin P Jung and Adriano Marchese and Regina Cheng and Henry H.Q Heng and Lee F Kolakowski and Kevin R Lynch and Susan R George
DOI: 10.1016/s0378-1119(96)00722-6
03/1997

Discovery of a Novel Human G Protein-Coupled Receptor Gene (GPR25) Located on Chromosome 1
Biochemical and Biophysical Research Communications
Benjamin P. Jung and Tuan Nguyen and Lee F. Kolakowski and Kevin R. Lynch and Henry H.Q. Heng and Susan R. George and Brian F. O'Dowd
DOI: 10.1006/bbrc.1996.5828
01/1997

Characterization of a human gene related to genes encoding somatostatin receptors
FEBS Letters
Lee F. Kolakowski and Benjamin P. Jung and Tuan Nguyen and Michael P. Johnson and Kevin R. Lynch and Regina Cheng and Henry H.Q. Heng and Susan R. George and Brian F. O'Dowd
DOI: 10.1016/s0014-5793(96)01160-x
1996

Research

CALIPER
A Canada-wide Initiative to Close the Critical Gaps in Pediatric Reference Intervals

Research Group Members

Samantha Pawer, Undergraduate Student