Overview

CALIPER: A Canada-wide Initiative to Close the Critical Gaps in Pediatric Reference Intervals

The diagnosis and care of pediatric patients is critically dependent on laboratory test results. Physicians assess test results and consider diagnoses by comparing these results to ‘normal ranges’. The validity of these normal ranges may vary depending on the the age and gender of subjects used to derive them. Additionally, different test methods exist that produce numerically different results, such that results and normal ranges are not interchangeable. Normal ranges for pediatrics for many laboratory tests are inaccurate and outdated. Also, for those tests that have good pediatric normal ranges available, there has been difficulty and delay in laboratories to report them.

CALIPER is a Canada-wide initiative to develop, implement, and maintain accurate and up-to-date normal ranges for any laboratory tests performed on children. CALIPER collects and analyzes small blood samples from healthy Canadian children of all ethnicities so that the results benefit our diverse population. Seven pediatric hospitals from across Canada, including BC Children's Hospital, are collaborating on the CALIPER project. We are currently planning to expand efforts to study reference ranges in neonates.

Publications

Limitations and opportunities of whole blood bilirubin measurements by GEM premier 4000®
BMC Pediatrics
Li Wang, Arianne Y. K. Albert, Benjamin Jung, Keyvan Hadad, Martha E. Lyon, Melanie Basso
DOI: 10.1186/s12887-017-0842-8
03/2017

Utility of Ara h 2 sIgE levels to predict peanut allergy in Canadian children
The Journal of Allergy and Clinical Immunology: In Practice
Sara H. Leo, John M. Dean, Benjamin Jung, Boris Kuzeljevic, Edmond S. Chan
DOI: 10.1016/j.jaip.2015.04.001
11/2015

Case report: Hemolytic interference of a kinetic Jaffe creatinine method
Clinical Biochemistry
Dailin Li, Andre Mattman, Benjamin Jung, Adelina Lim
DOI: 10.1016/j.clinbiochem.2015.07.082
10/2015

Negative heterophile antibody interference affecting iPTH
Clinical Biochemistry
Andre Mattman, Benjamin Jung, Kristin Hauff, Laura Burns, Daniel Holmes, Supna Sandhu, Breay Paty
DOI: 10.1016/j.clinbiochem.2015.07.084
10/2015

Acute encephalopathy in a kidney transplant recipient following infusion of intravenous immunoglobulin
Transplant International
Li Wang, Nicholas Larkins, Benjamin Jung, Nicholas H.C. Au, Cherry Mammen
DOI: 10.1111/tri.12384
08/2014

Comparison of the Nova StatStrip(R) Lactate versus the Arkray Lactate Pro(R) meters for fetal scalp lactate measurement
Clinical Biochemistry
Melanie Basso, Benjamin Jung, Li Wang, Elvira Kozak, Ivy Fernando, Catherine Halstead, Ellen Giesbrecht
DOI: 10.1016/j.clinbiochem.2014.06.036
08/2014

Acute encephalopathy due to true hyponatremia and hyperammonemia after fast infusion of intravenous immunoglobulin
Clinical Biochemistry
L. Wang, N. Larkins, B. Jung, N. Au, C. Mammen
DOI: 10.1016/j.clinbiochem.2014.04.077
06/2014

CLSI-based transference of the CALIPER database of pediatric reference intervals from Abbott to Beckman, Ortho, Roche and Siemens Clinical Chemistry Assays: Direct validation using reference samples from the CALIPER cohort
Clinical Biochemistry
Mathew P. Estey, Ashley H. Cohen, David A. Colantonio, Man Khun Chan, Tina Binesh Marvasti, Edward Randell, Edgard Delvin, Jocelyne Cousineau, Vijaylaxmi Grey, Donald Greenway, Qing H. Meng, Benjamin Jung, Jalaluddin Bhuiyan, David Seccombe, Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2013.04.001
09/2013

Discovery of undocumented drug interferences on the Vitros urine methadone immunoassay
Clinical Biochemistry
M. Seyed Ibrahim, R. Saeedi, W. Martz, J. Bhuiyan, B. Jung, A.C. Halstead
DOI: 10.1016/j.clinbiochem.2012.07.056
09/2012

Evaluation of the Sebia isoelectric focusing method for alpha 1 antripsin phenotype determination
Clinical Biochemistry
T.N. Higgins, M. Limcango, B. Jung
DOI: 10.1016/j.clinbiochem.2012.07.036
09/2012

Validation of the Randox total bile acids assay on the Vitros 5600
Clinical Biochemistry
R. Saeedi, M. Seyed Ibrahim, L. Trowski, B. Jung, J. Bhuiyan, A.C. Halstead
DOI: 10.1016/j.clinbiochem.2012.07.055
09/2012

Sanger sequencing solved a cryptic case of severe alpha1-antitrypsin deficiency
Clinical Biochemistry
Shing H. Zhan, Raja T. Abboud, Benjamin Jung, Brian Kuchinka, Diana Ralston, Brett Casey, Andre Mattman
DOI: 10.1016/j.clinbiochem.2012.01.027
04/2012

Alpha1-antitrypsin deficiency: a clinical-genetic overview
The Application of Clinical Genetics
Raja Abboud, Nelson, Tanya, Benjamin Jung, Andre Mattman
DOI: 10.2147/tacg.s10604
03/2011

Pediatric reference intervals for 28 chemistries and immunoassays on the Roche cobas® 6000 analyzer—A CALIPER pilot study
Clinical Biochemistry
Vathany Kulasingam, Benjamin P. Jung, Ivan M. Blasutig, Sanaz Baradaran, Man Khun Chan, Mujdat Aytekin, David A. Colantonio, Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2010.05.008
09/2010

Analytical evaluation of the VITROS® 5600 Integrated System in a pediatric setting and determination of pediatric reference intervals
Clinical Biochemistry
Ivan M. Blasutig, Benjamin Jung, Vathany Kulasingam, Sanaz Baradaran, Yu Chen, Man Khun Chan, David Colantonio, Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2010.05.011
09/2010

Pediatric reference intervals for 28 biochemistry analytes on the Roche cobas® 6000 analyzer
Clinical Biochemistry
V. Kulasingam, B.P. Jung, I.M. Blasutig, S. Baradaran, M.K. Chan, M. Aytekin, D.A. Colantonio, K. Adeli
DOI: 10.1016/j.clinbiochem.2010.04.007
06/2010

Clinical laboratory reference intervals in pediatrics: The CALIPER initiative
Clinical Biochemistry
Benjamin Jung, Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2009.06.025
11/2009

Identification of a murine methyl cytosine phosphate guanine binding domain-containing factor 3 (MBD3) promoter segment sufficient for driving reporter gene expression in neurons in vitro and in vivo
Neuroscience
B.P. Jung, D. Purushotham, J.H. Eubanks
DOI: 10.1016/j.neuroscience.2006.12.057
03/2007

Isolation and characterization of LCHN: a novel factor induced by transient global ischemia in the adult rat hippocampusIdentification of a novel ischemia-induced gene
Journal of Neurochemistry
Guangming Zhang, Benjamin P. Jung, Warren Ho, Denis G. M. Jugloff, Herman H. Cheung, James W. Gurd, M. Christopher Wallace, James H. Eubanks
DOI: 10.1111/j.1471-4159.2006.04374.x
11/2006

Increased dendritic complexity and axonal length in cultured mouse cortical neurons overexpressing methyl-CpG-binding protein MeCP2
Neurobiology of Disease
Denis G.M. Jugloff, Benjamin P. Jung, Diana Purushotham, Richard Logan, James H. Eubanks
DOI: 10.1016/j.nbd.2004.11.002
06/2005

Decreased expression and impaired function of muscarinic acetylcholine receptors in the rat hippocampus following transient forebrain ischemia
Neurobiology of Disease
Guangming Zhang, Liang Zhang, Richard Logan, Benjamin P. Jung, Darren Yuen, Jee Ching Hsu, James H. Eubanks
DOI: 10.1016/j.nbd.2005.05.011
2005

Differential expression of methyl CpG-binding domain containing factor MBD3 in the developing and adult rat brain
Journal of Neurobiology
Benjamin P. Jung, Guangming Zhang, Romy Nitsch, Judy Trogadis, Sukriti Nag, James H. Eubanks
DOI: 10.1002/neu.10199
05/2003

The expression of methyl CpG binding factor MeCP2 correlates with cellular differentiation in the developing rat brain and in cultured cells
Journal of Neurobiology
Benjamin P. Jung, Denis G.M. Jugloff, Guangming Zhang, Richard Logan, Stephanie Brown, James H. Eubanks
DOI: 10.1002/neu.10201
02/2003

Expression and function of A1 adenosine receptors in the rat hippocampus following transient forebrain ischemia
Neuroscience
H Shen, L Zhang, D Yuen, R Logan, B.P Jung, G Zhang, J.H Eubanks
DOI: 10.1016/s0306-4522(02)00352-4
10/2002

Kindling induces the mRNA expression of methyl DNA-binding factors in the adult rat hippocampus
Neuroscience
J Francis, B Jung, G Zhang, J Cheng, W Ho, W.M Burnham, J.H Eubanks
DOI: 10.1016/s0306-4522(02)00150-1
08/2002

Transient forebrain ischemia alters the mRNA expression of methyl DNA-binding factors in the adult rat hippocampus
Neuroscience
B.P Jung, G Zhang, W Ho, J Francis, J.H Eubanks
DOI: 10.1016/s0306-4522(02)00383-4
2002

Perforant pathway kindling transiently induces the mRNA expression of GABA-B receptor subtypes R1A and R2 in the adult rat hippocampus
Molecular Brain Research
Joseph Francis, Benjamin P. Jung, Guangming Zhang, Warren Ho, Jerome Cheng, W. McIntyre Burnham, James H. Eubanks
DOI: 10.1016/s0169-328x(01)00122-x
07/2001

Somatostatin type 2 receptor expression in the rat hippocampus following cerebral ischemia
Neuroreport
Warren Ho, Benjamin P. Jung, Guangming Zhang, James H. Eubanks
DOI: 10.1097/00001756-200107200-00013
07/2001

Differential increase in Fos immunoreactivity in hypothalamic and septal nuclei by arginine8-vasopressin and desglycinamide9-arginine8-vasopressin
Neuroscience
A.J Lança, P.H Wu, B Jung, J.-F Liu, V Ng, H Kalant
DOI: 10.1016/s0306-4522(98)00709-x
07/1999

Cloning and chromosomal mapping of four putative novel human G-protein-coupled receptor genes
Gene
Brian F O'Dowd, Tuan Nguyen, Benjamin P Jung, Adriano Marchese, Regina Cheng, Henry H.Q Heng, Lee F Kolakowski, Kevin R Lynch, Susan R George
DOI: 10.1016/s0378-1119(96)00722-6
03/1997

Discovery of a Novel Human G Protein-Coupled Receptor Gene (GPR25) Located on Chromosome 1
Biochemical and Biophysical Research Communications
Benjamin P. Jung, Tuan Nguyen, Lee F. Kolakowski, Kevin R. Lynch, Henry H.Q. Heng, Susan R. George, Brian F. O'Dowd
DOI: 10.1006/bbrc.1996.5828
01/1997

Characterization of a human gene related to genes encoding somatostatin receptors
FEBS Letters
Lee F. Kolakowski, Benjamin P. Jung, Tuan Nguyen, Michael P. Johnson, Kevin R. Lynch, Regina Cheng, Henry H.Q. Heng, Susan R. George, Brian F. O'Dowd
DOI: 10.1016/s0014-5793(96)01160-x
1996

Research

CALIPER
A Canada-wide Initiative to Close the Critical Gaps in Pediatric Reference Intervals

Research Group Members

Samantha Pawer, Undergraduate Student