CALIPER: A Canada-wide Initiative to Close the Critical Gaps in Pediatric Reference Intervals

The diagnosis and care of pediatric patients is critically dependent on laboratory test results. Physicians assess test results and consider diagnoses by comparing these results to ‘normal ranges’. The validity of these normal ranges may vary depending on the the age and gender of subjects used to derive them. Additionally, different test methods exist that produce numerically different results, such that results and normal ranges are not interchangeable. Normal ranges for pediatrics for many laboratory tests are inaccurate and outdated. Also, for those tests that have good pediatric normal ranges available, there has been difficulty and delay in laboratories to report them.

CALIPER is a Canada-wide initiative to develop, implement, and maintain accurate and up-to-date normal ranges for any laboratory tests performed on children. CALIPER collects and analyzes small blood samples from healthy Canadian children of all ethnicities so that the results benefit our diverse population. Seven pediatric hospitals from across Canada, including BC Children's Hospital, are collaborating on the CALIPER project. We are currently planning to expand efforts to study reference ranges in neonates.


Limitations and opportunities of whole blood bilirubin measurements by GEM premier 4000®
BMC Pediatrics
Li Wang, Arianne Y. K. Albert, Benjamin Jung, Keyvan Hadad, Martha E. Lyon, Melanie Basso
DOI: 10.1186/s12887-017-0842-8

Utility of Ara h 2 sIgE levels to predict peanut allergy in Canadian children
The Journal of Allergy and Clinical Immunology: In Practice
Sara H. Leo, John M. Dean, Benjamin Jung, Boris Kuzeljevic, Edmond S. Chan
DOI: 10.1016/j.jaip.2015.04.001

Case report: Hemolytic interference of a kinetic Jaffe creatinine method
Clinical Biochemistry
Dailin Li, Andre Mattman, Benjamin Jung, Adelina Lim
DOI: 10.1016/j.clinbiochem.2015.07.082

Negative heterophile antibody interference affecting iPTH
Clinical Biochemistry
Andre Mattman, Benjamin Jung, Kristin Hauff, Laura Burns, Daniel Holmes, Supna Sandhu, Breay Paty
DOI: 10.1016/j.clinbiochem.2015.07.084

Acute encephalopathy in a kidney transplant recipient following infusion of intravenous immunoglobulin
Transplant International
Li Wang, Nicholas Larkins, Benjamin Jung, Nicholas H.C. Au, Cherry Mammen
DOI: 10.1111/tri.12384

Comparison of the Nova StatStrip(R) Lactate versus the Arkray Lactate Pro(R) meters for fetal scalp lactate measurement
Clinical Biochemistry
Melanie Basso, Benjamin Jung, Li Wang, Elvira Kozak, Ivy Fernando, Catherine Halstead, Ellen Giesbrecht
DOI: 10.1016/j.clinbiochem.2014.06.036

Acute encephalopathy due to true hyponatremia and hyperammonemia after fast infusion of intravenous immunoglobulin
Clinical Biochemistry
L. Wang, N. Larkins, B. Jung, N. Au, C. Mammen
DOI: 10.1016/j.clinbiochem.2014.04.077

CLSI-based transference of the CALIPER database of pediatric reference intervals from Abbott to Beckman, Ortho, Roche and Siemens Clinical Chemistry Assays: Direct validation using reference samples from the CALIPER cohort
Clinical Biochemistry
Mathew P. Estey, Ashley H. Cohen, David A. Colantonio, Man Khun Chan, Tina Binesh Marvasti, Edward Randell, Edgard Delvin, Jocelyne Cousineau, Vijaylaxmi Grey, Donald Greenway, Qing H. Meng, Benjamin Jung, Jalaluddin Bhuiyan, David Seccombe, Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2013.04.001

Discovery of undocumented drug interferences on the Vitros urine methadone immunoassay
Clinical Biochemistry
M. Seyed Ibrahim, R. Saeedi, W. Martz, J. Bhuiyan, B. Jung, A.C. Halstead
DOI: 10.1016/j.clinbiochem.2012.07.056

Evaluation of the Sebia isoelectric focusing method for alpha 1 antripsin phenotype determination
Clinical Biochemistry
T.N. Higgins, M. Limcango, B. Jung
DOI: 10.1016/j.clinbiochem.2012.07.036

Validation of the Randox total bile acids assay on the Vitros 5600
Clinical Biochemistry
R. Saeedi, M. Seyed Ibrahim, L. Trowski, B. Jung, J. Bhuiyan, A.C. Halstead
DOI: 10.1016/j.clinbiochem.2012.07.055

Sanger sequencing solved a cryptic case of severe alpha1-antitrypsin deficiency
Clinical Biochemistry
Shing H. Zhan, Raja T. Abboud, Benjamin Jung, Brian Kuchinka, Diana Ralston, Brett Casey, Andre Mattman
DOI: 10.1016/j.clinbiochem.2012.01.027

Alpha1-antitrypsin deficiency: a clinical-genetic overview
The Application of Clinical Genetics
Raja Abboud, Nelson, Tanya, Benjamin Jung, Andre Mattman
DOI: 10.2147/tacg.s10604

Pediatric reference intervals for 28 chemistries and immunoassays on the Roche cobas® 6000 analyzer—A CALIPER pilot study
Clinical Biochemistry
Vathany Kulasingam, Benjamin P. Jung, Ivan M. Blasutig, Sanaz Baradaran, Man Khun Chan, Mujdat Aytekin, David A. Colantonio, Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2010.05.008

Analytical evaluation of the VITROS® 5600 Integrated System in a pediatric setting and determination of pediatric reference intervals
Clinical Biochemistry
Ivan M. Blasutig, Benjamin Jung, Vathany Kulasingam, Sanaz Baradaran, Yu Chen, Man Khun Chan, David Colantonio, Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2010.05.011

Pediatric reference intervals for 28 biochemistry analytes on the Roche cobas® 6000 analyzer
Clinical Biochemistry
V. Kulasingam, B.P. Jung, I.M. Blasutig, S. Baradaran, M.K. Chan, M. Aytekin, D.A. Colantonio, K. Adeli
DOI: 10.1016/j.clinbiochem.2010.04.007

Clinical laboratory reference intervals in pediatrics: The CALIPER initiative
Clinical Biochemistry
Benjamin Jung, Khosrow Adeli
DOI: 10.1016/j.clinbiochem.2009.06.025

Identification of a murine methyl cytosine phosphate guanine binding domain-containing factor 3 (MBD3) promoter segment sufficient for driving reporter gene expression in neurons in vitro and in vivo
B.P. Jung, D. Purushotham, J.H. Eubanks
DOI: 10.1016/j.neuroscience.2006.12.057

Isolation and characterization of LCHN: a novel factor induced by transient global ischemia in the adult rat hippocampusIdentification of a novel ischemia-induced gene
Journal of Neurochemistry
Guangming Zhang, Benjamin P. Jung, Warren Ho, Denis G. M. Jugloff, Herman H. Cheung, James W. Gurd, M. Christopher Wallace, James H. Eubanks
DOI: 10.1111/j.1471-4159.2006.04374.x

Increased dendritic complexity and axonal length in cultured mouse cortical neurons overexpressing methyl-CpG-binding protein MeCP2
Neurobiology of Disease
Denis G.M. Jugloff, Benjamin P. Jung, Diana Purushotham, Richard Logan, James H. Eubanks
DOI: 10.1016/j.nbd.2004.11.002

Decreased expression and impaired function of muscarinic acetylcholine receptors in the rat hippocampus following transient forebrain ischemia
Neurobiology of Disease
Guangming Zhang, Liang Zhang, Richard Logan, Benjamin P. Jung, Darren Yuen, Jee Ching Hsu, James H. Eubanks
DOI: 10.1016/j.nbd.2005.05.011

Differential expression of methyl CpG-binding domain containing factor MBD3 in the developing and adult rat brain
Journal of Neurobiology
Benjamin P. Jung, Guangming Zhang, Romy Nitsch, Judy Trogadis, Sukriti Nag, James H. Eubanks
DOI: 10.1002/neu.10199

The expression of methyl CpG binding factor MeCP2 correlates with cellular differentiation in the developing rat brain and in cultured cells
Journal of Neurobiology
Benjamin P. Jung, Denis G.M. Jugloff, Guangming Zhang, Richard Logan, Stephanie Brown, James H. Eubanks
DOI: 10.1002/neu.10201

Expression and function of A1 adenosine receptors in the rat hippocampus following transient forebrain ischemia
H Shen, L Zhang, D Yuen, R Logan, B.P Jung, G Zhang, J.H Eubanks
DOI: 10.1016/s0306-4522(02)00352-4

Kindling induces the mRNA expression of methyl DNA-binding factors in the adult rat hippocampus
J Francis, B Jung, G Zhang, J Cheng, W Ho, W.M Burnham, J.H Eubanks
DOI: 10.1016/s0306-4522(02)00150-1

Transient forebrain ischemia alters the mRNA expression of methyl DNA-binding factors in the adult rat hippocampus
B.P Jung, G Zhang, W Ho, J Francis, J.H Eubanks
DOI: 10.1016/s0306-4522(02)00383-4

Perforant pathway kindling transiently induces the mRNA expression of GABA-B receptor subtypes R1A and R2 in the adult rat hippocampus
Molecular Brain Research
Joseph Francis, Benjamin P. Jung, Guangming Zhang, Warren Ho, Jerome Cheng, W. McIntyre Burnham, James H. Eubanks
DOI: 10.1016/s0169-328x(01)00122-x

Somatostatin type 2 receptor expression in the rat hippocampus following cerebral ischemia
Warren Ho, Benjamin P. Jung, Guangming Zhang, James H. Eubanks
DOI: 10.1097/00001756-200107200-00013

Differential increase in Fos immunoreactivity in hypothalamic and septal nuclei by arginine8-vasopressin and desglycinamide9-arginine8-vasopressin
A.J Lança, P.H Wu, B Jung, J.-F Liu, V Ng, H Kalant
DOI: 10.1016/s0306-4522(98)00709-x

Cloning and chromosomal mapping of four putative novel human G-protein-coupled receptor genes
Brian F O'Dowd, Tuan Nguyen, Benjamin P Jung, Adriano Marchese, Regina Cheng, Henry H.Q Heng, Lee F Kolakowski, Kevin R Lynch, Susan R George
DOI: 10.1016/s0378-1119(96)00722-6

Discovery of a Novel Human G Protein-Coupled Receptor Gene (GPR25) Located on Chromosome 1
Biochemical and Biophysical Research Communications
Benjamin P. Jung, Tuan Nguyen, Lee F. Kolakowski, Kevin R. Lynch, Henry H.Q. Heng, Susan R. George, Brian F. O'Dowd
DOI: 10.1006/bbrc.1996.5828

Characterization of a human gene related to genes encoding somatostatin receptors
FEBS Letters
Lee F. Kolakowski, Benjamin P. Jung, Tuan Nguyen, Michael P. Johnson, Kevin R. Lynch, Regina Cheng, Henry H.Q. Heng, Susan R. George, Brian F. O'Dowd
DOI: 10.1016/s0014-5793(96)01160-x


A Canada-wide Initiative to Close the Critical Gaps in Pediatric Reference Intervals

Research Group Members

Samantha Pawer, Undergraduate Student