Lipid analysis

Our laboratory has extensive experience with lipid analysis and can provide identification and quantification of individual lipids and fatty acids present in a variety of samples, including cells, tissues, fluids and foods.

Analyses include: 

Fatty Acyls: Total fatty acids and separation, identification and quantification of individual fatty acids including saturated, monounsaturated, omega-6 and omega-3 fatty acids, conjugated, trans and cis-trans isomers with carbon chain lengths from 8-24. Quantitation of fatty acids on a weight percent basis employs internal standards added to samples prior to extraction.

Glycerophospholipids, glycerolipids, sphingolipids and sterols:

  • Total phospholipids and individual phospholipids including phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol, cardiolipin
  • Triglycerides and partial glycerides
  • Sphingomyelin
  • Sterol esters, cholesterol, cholesterol precursors and plant sterols

Sample Requirement:

Samples can be from any animal, plant or food source. Examples are plasma, serum, red cells, white cells, milk, saliva, tissues, cells, membrane fractions. Robotic sample handling enables a high degree of accuracy and reproducibility with low sample volumes. For example, quantitative analysis of fatty acids is possible in as little as 80 µL of plasma and quantitative analysis of phospholipids and triglyceride is possible with 200 µL of plasma. The minimum amount of sample required will depend on the analysis requested.

Laboratory Instrumentation:

Agilent 6850 gas chromatographs equipped with flame ionization detection, equipped with 30 and 100m capillary columns. These instruments are dedicated for analysis of qualitative and quantitative analysis of fatty acids on plasma, cell, specific membrane fractions, or milk and foods stuff. Analysis include medium and long chain saturated fatty acids, monounsaturated fatty acids, n-6 and n-3 polyunsaturated, as well as conjugated, tans and cis-trans isomers.

Waters Alliance 2695 high pressure liquid chromatograph, with temperature controlled autosampler and column holder, Alltech Evaporative Light Scattering Detector and Gilson fraction collector. This instrument is dedicated to targeted lipidomics and lipid profiling of a wide range of lipids. Used for separation and quantification of lipid classes from plasma or other biological materials.

Methyl and one-carbon metabolites

Our laboratory offers quantification of methyl and one-carbon metabolites using HPLC-tandem mass spectrometry, ELISA, radioimmunoassay (RIA) and ion capture in a variety of samples, including cells, tissues, and fluids from adults, infants and animal or cell models. 

Analyses include:

  • AnalysesHomocysteine
  • Methionine
  • S-adenosyl homocysteine (SAH)
  • S-adenosyl methionine (SAM)
  • Cysteine
  • Choline
  • Betaine
  • Dimethylglycine (DMG)
  • Oxoproline
  • Taurine
  • Acetylcholine
  • Folate (plasma and red blood cell)
  • Vitamin B12
  • Vitamin D
  • Other assays upon request

Sample Requirement:

Sample volume will depend on the number of analyses required. Most analyses require small volumes, for example 20 µL for choline, betaine, and dimethylglycine. Analyses of SAM and SAH require sepcition procedure during sample preparation. 

Laboratory Equipment:

  • A Quattro micro triple-quadrupole MS system configured with an electrospray source coupled to an Acquity HPLC equipped with a thermostatted autosampler, operated in both positive and negative ion detection mode and used for quantification of selected metabolites. 
  • AxSYM (Abbott) automated analyzer for immunodiagnostic tests. Processes a variety of tests simultaneously, with up to 120 tests per hour.

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