• Brown, Lindsay

    Investigator, BC Children's Hospital
    Clinical Associate Professor, Department of Pathology and Laboratory Medicine, Faculty of Medicine, University of British Columbia
    Degrees / Designations
    PhD, FCCMG 
    Primary Area of Research
    Childhood Diseases
    Secondary Area(s) of Research
    604-875-2000 ext. 7534
    Lab Phone
    Mailing Address
    Department of Pathology
    Cytogenetics Lab
    4500 Oak Street
    Vancouver, BC  V6H 3N1
    Affiliate Websites
    Research Areas
    • Clinical cytogenetics 
    • Cancer genetics: Pediatric leukemia and lymphomas, and solid tumors 

    My current research focuses on exploring chromosome abnormalities in patients diagnosed with acute leukemia.

    Acute leukemia is the most common cancer affecting children. Although the outcome of treatment has improved substantially in recent decades, there remains approximately 20 per cent of children treated for leukemia who relapse and have a poor prognosis. 

    My research aims to use high resolution microarray analysis to better identify the genetic changes associated with acute leukemia. I am particularly interested in determining if there are genetic alterations unique to treatment-resistant leukemia. Obtaining a detailed understanding of the genetics of acute leukemia will enable other researchers to develop targeted, personalized treatments with greater success rates and less harmful effects. 

    Current Projects

    Acute leukemia has been well characterized genetically, largely due to the successful implementation of cytogenetic techniques to identify large chromosomal abnormalities, the detection of which has proven important in terms of diagnosis, prognosis and treatment. There has been an effort to use high resolution genomic profiling to identify novel genetic alterations that contribute to leukemogenesis. 

    Several microarray-based techniques for the genome-wide assessment of DNA copy number alterations (CNA) are now routinely used in the study of cancer genetics. Currently, the Cytogenetics Laboratory at C&W uses numerous cyotogenetic techniques, including karyotype, fluorescent in-situ hybridization, and more recently microarray to provide a comprehensive genomic analysis of pediatric acute leukemia. The power of microarray is that it can identify small CNA's and well as regions of copy number-neutral loss of heterozygosity (CN-LOH). 

    Currently, all leukemia’s are evaluated in the Cytogenetics laboratory and CMA is performed. Established clinical thresholds are used for identifying CNAs. The aim of the current research project is to furhter explore the existing data looking at much smaller CNAs (outside of the clinical thresholds) and assess CN-LOH. The use of SNP-arrays to identify somatically altered regions of the genome is anticipated to lead to the discovery of clinically relevant genes involved in leukemogenesis.

    Selected Publications
    1. Schlade-Bartusiak K, Brown L, Lomax B, Bruyère H, Gillan T, Hamilton S, McGillivray B, Eydoux P. BPES with atypical premature ovarian insufficiency, and evidence of mitotic recombination, in a woman with trisomy X and a translocation t(3;11)(q22.3;q14.1). Am J Med Genet A. 2012 Sep;158A(9):2322-7.
    2. Tucker T, Schlade-Bartusiak K, Eydoux P, Nelson T, Brown L: Uniparental disomy: can SNP array data be used for diagnosis? Accepted in Genet Med.
    3. Tucker T, Nelson T, Sirrs S, Roughley P, Glorieux FH, Moffatt P, Schlade-Bartusiak K, Brown L, Rauch F: A Co-Occurrence of Osteogenesis Imperfecta Type VI and Cystinosis. Accepted in Am J Med Genet.
    4. Falconer E, Chavez EA, Henderson A, Poon SS, McKinney S, Brown L, Huntsman DG, Lansdorp PM.  Identification of sister chromatids by DNA template strand sequences.  Nature. 2010 Jan 7:463(7277):93-7.
    5. Brown LA, Johnson K,  Leung S, Bismar TA,Benítez J, Foulkes WD, Huntsman DG. Co-amplification of CCND1 and EMSY is associated with an adverse outcome in ER-positive tamoxifen-treated breast cancers. Breast Cancer Res Treat. 2009 Jul 28
    6. Brown LA, Hoog J, Chin SF, Tao Y, Zayed AA, Chin K, Teschendorff AE, Quackenbush JF, Marioni JC, Leung S, Perou CM, Neilsen TO, Ellis M, Gray JW, Bernard PS, Huntsman DG, Caldas C.  ESR1 amplification in breast cancer: a common phenomenon? Nature Genetics. 2008 Jul;40(7):806-7.
    7. Brown LA, Kalloger SE, Miller MA, Shih IeM, McKinney SE, Santos JL, Swenerton K, Spellman PT, Gray J, Gilks CB, Huntsman DG.  Amplification of 11q13 in ovarian carcinoma.  Genes Chromosomes Cancer. 2008 Jun;47(6):481-9.
    8. Hewitt J, Craven SJ, Brown LA, Bleackley MR, Ballard JN, Smith VC, Ofosu FA, Huntsman DG, Wadsworth LD, Wu JK, Macgillivray RT.   Molecular determination of the breakpoints of a 161 556 bp deletion at chromosome 13q34 that presented as severe  factor VII deficiency in a neonate.  Br J Haematol. 2008 Mar;140(5):589-92.
    • BCCH Telethon Award 2012, Genome wide assessment of genetic alterations in pediatric acute lymphoblastic leukemia
    • BCCH Telethon Award 2010, Discovery of novel candidate genes involved in facial clefting: A pilot project 

    Honours & Awards
    • CCMG Fellowship Salary Award, UBC, 2008
    • Linda Stevens Memorial Fund (CCMG), 2008
    • Travel Fellowship for the Helene Harris Memorial Trust 11th International Forum on Ovarian Cancer, Lake Como, Italy, 2007
    • Best Oral Presentation, UBC Pathology Day, 2006
    • Stowell-Orbison Award Certificate of Merit, USCAP 2004 Annual Meeting
    • Selected for AACR Pathobiology of Cancer Workshop, 2004
    • Best Clinical Poster, UBC Pathology Day, 2004
    • Best Clinical Poster, UBC Pathology Day, 2002     
    Research Group Members