Publications

Performance of the Alethia CMV Assay for Detection of Cytomegalovirus by Use of Neonatal Saliva Swabs
Journal of Clinical Microbiology
Soren Gantt and David M. Goldfarb and Albert Park and William Rawlinson and Suresh B. Boppana and Tiziana Lazzarotto and Lawrence M. Mertz
DOI: 10.1128/JCM.01951-19
01/2020

AMMI Canada: Response to FAQs about the management of children with congenital cytomegalovirus infection in Canada
Official Journal of the Association of Medical Microbiology and Infectious Disease Canada
DOI: 10.3138/jammi.2019-08-21
10/2019

Valganciclovir prophylaxis delays onset of EBV viremia in high-risk pediatric solid organ transplant recipients
Pediatric Research
DOI: 10.1038/s41390-019-0523-4
08/2019

Anti-Factor H Antibody Reactivity in Young Adults Vaccinated with a Meningococcal Serogroup B Vaccine Containing Factor H Binding Protein
mSphere
DOI: 10.1128/msphere.00393-19
07/2019

Humoral Immune Correlates for Prevention of Postnatal Cytomegalovirus Acquisition.
The Journal of infectious diseases
Saccoccio FM and Jenks JA and Itell HL and Li SH and Berry M and Pollara J and Casper C and Gantt S and Permar SR
DOI: 10.1093/infdis/jiz192
PubMed: 31107951
07/2019

Increased oral Epstein-Barr virus shedding with HIV-1 co-infection is due to a combination of B cell activation and impaired cellular immune control
Byrne CM and Johnston C and Orem J and Okuku F and Huang M and Selke S and Wald A and Corey L and Schiffer JT and Casper C and Coombs D and Gantt S
DOI: 10.1101/587063
03/2019

116. Role of Maternal Antibodies in Protection Against Postnatal Cytomegalovirus Acquisition.
Open Forum Infectious Diseases
Saccoccio F and Itell H and Li S and Jenks J and Xie G and Pollara J and Gantt S and Permar S
DOI: 10.1093/ofid/ofy209.007
11/2018

LB18. An Enveloped Virus-like Particle (eVLP) Cytomegalovirus (CMV) Vaccine Is Immunogenic and Safe: Results of a First-in-Humans Study.
Open forum infectious diseases
Gantt S and Quach C and Anderson DE and Diaz-Mitoma F and Langley J
DOI: 10.1093/ofid/ofy229.2192
11/2018

Natural history of postnatal rhesus cytomegalovirus shedding by dams and acquisition by infant rhesus monkeys
PLOS ONE
Amitinder Kaur and Hannah L. Itell and E. Peek Ehlinger and Valerie Varner and Soren Gantt and Sallie R. Permar
DOI: 10.1371/journal.pone.0206330
10/2018

In reference to should infants who fail their newborn hearing screen undergo cytomegalovirus testing ?
The Laryngoscope
Soren Gantt and David M. Goldfarb and Francois Dionne and Dennis Bulman and Sara Menlove Doutré
DOI: 10.1002/lary.27032
07/2018

Nonprimary Maternal Cytomegalovirus Infection After Viral Shedding in Infants.
The Pediatric infectious disease journal
DOI: 10.1097/INF.0000000000001877
PubMed: 29889809
07/2018

Higher Expectations for a Vaccine To Prevent Congenital Cytomegalovirus Infection
Journal of Virology
Soren Gantt and Arnaud Marchant and Suresh B. Boppana
DOI: 10.1128/jvi.00764-18
07/2018

Recovery of cytomegalovirus DNA from newborn saliva samples by different methods
Journal of Clinical Virology
Oran Goshen and David M. Goldfarb and Laura Book and Peter Tilley and Soren Gantt
DOI: 10.1016/j.jcv.2018.05.001
07/2018

Effects of spatiotemporal HSV-2 lesion dynamics and antiviral treatment on the risk of HIV-1 acquisition
PLOS Computational Biology
Catherine M. Byrne and Soren Gantt and Daniel Coombs
DOI: 10.1371/journal.pcbi.1006129
04/2018

Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes.
Scientific reports
DOI: 10.1038/s41598-018-23930-1
PubMed: 29615801
04/2018

Successful treatment of chronic norovirus gastroenteritis with nitazoxanide in a pediatric kidney transplant recipient
Pediatric Transplantation
Kathryn Haubrich and Soren Gantt and Tom Blydt-Hansen
DOI: 10.1111/petr.13186
04/2018

The Potential Harm of Cytomegalovirus Infection in Immunocompetent Critically Ill Children
Frontiers in Pediatrics
Raidan Alyazidi and Srinivas Murthy and Jennifer A. Slyker and Soren Gantt
DOI: 10.3389/fped.2018.00096
04/2018

Comparative genomic, transcriptomic, and proteomic reannotation of human herpesvirus 6.
BMC genomics
DOI: 10.1186/s12864-018-4604-2
PubMed: 29554870
03/2018

KSHV oral shedding and plasma viremia result in significant changes in the extracellular tumorigenic miRNA expression profile in individuals infected with the malaria parasite.
PloS one
DOI: 10.1371/journal.pone.0192659
PubMed: 29425228
02/2018

Herpes simplex Virus
Oxford Medicine Online
Raidan Alyazidi and Soren Gantt
DOI: 10.1093/med/9780190604813.003.0007
01/2018

Toll-like receptor 9 polymorphism is associated with increased Epstein-Barr virus and Cytomegalovirus acquisition in HIV-exposed infants.
AIDS (London, England)
DOI: 10.1097/QAD.0000000000001680
PubMed: 29112074
01/2018

Virus and host-specific differences in oral human herpesvirus shedding kinetics among Ugandan women and children.
Scientific reports
DOI: 10.1038/s41598-017-12994-0
PubMed: 29026166
10/2017

Reply to “Early Stochastic Dynamics in Human Cytomegalovirus Infection”
Journal of Virology
Soren Gantt and Bryan T. Mayer and Joshua T. Schiffer
DOI: 10.1128/jvi.01006-17
08/2017

Genomic and proteomic analysis of Human herpesvirus 6 reveals distinct clustering of acute versus inherited forms and reannotation of reference strain
Greninger AL and Knudsen GM and Roychoudhury P and Hanson DJ and Sedlak RH and Xie H and Guan J and Nguyen T and Peddu V and Boeckh M and Huang M and Cook L and Depledge DP and Zerr DM and Jerome KR
DOI: 10.1101/181248
08/2017

Transient Oral Human Cytomegalovirus Infections Indicate Inefficient Viral Spread from Very Few Initially Infected Cells.
Journal of virology
DOI: 10.1128/JVI.00380-17
PubMed: 28381570
05/2017

Diagnosis and management of infants with congenital cytomegalovirus infection
Paediatrics & Child Health
Soren Gantt and Ari Bitnun and Christian Renaud and Fatima Kakkar and Wendy Vaudry
DOI: 10.1093/pch/pxx002
04/2017

Cost-effectiveness of Universal and Targeted Newborn Screening for Congenital Cytomegalovirus Infection.
JAMA pediatrics
DOI: 10.1001/jamapediatrics.2016.2016
PubMed: 27723885
12/2016

Dynamics of Persistent Oral Cytomegalovirus Shedding During Primary Infection in Ugandan Infants.
The Journal of infectious diseases
DOI: 10.1093/infdis/jiw442
PubMed: 27651417
09/2016

Diagnosis and management of congenital cytomegalovirus infection
This Changed My Practice
06/2016

Bacillus pumilus Septic Arthritis in a Healthy Child.
The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale
DOI: 10.1155/2016/3265037
PubMed: 27366165
04/2016

Symptomatic Congenital Cytomegalovirus Infection Is Underdiagnosed in British Columbia
The Journal of Pediatrics
Brendan Sorichetti and Oran Goshen and Julie Pauwels and Frederick K. Kozak and Peter Tilley and Mel Krajden and Soren Gantt
DOI: 10.1016/j.jpeds.2015.10.069
02/2016

Patterns of human herpesvirus-8 oral shedding among diverse cohorts of human herpesvirus-8 seropositive persons
Infect Agents Cancer
Rachel A. Bender Ignacio and Jason D. Goldman and Amalia S. Magaret and Stacy Selke and Meei-Li Huang and Soren Gantt and Christine Johnston and Warren T. Phipps and Joshua T. Schiffer and Richard A. Zuckerman and R. Scott McClelland and Connie Celum and Larry Corey and Anna Wald and Corey Casper
DOI: 10.1186/s13027-016-0052-2
02/2016

Prospective Characterization of the Risk Factors for Transmission and Symptoms of Primary Human Herpesvirus Infections Among Ugandan Infants
J Infect Dis.
Soren Gantt and Jackson Orem and Elizabeth M. Krantz and Rhoda Ashley Morrow and Stacy Selke and Meei-Li Huang and Joshua T. Schiffer and Keith R. Jerome and Annet Nakaganda and Anna Wald and Corey Casper and Lawrence Corey
DOI: 10.1093/infdis/jiw076
02/2016

Risk of congenital cytomegalovirus infection among HIV-exposed uninfected infants is not decreased by maternal nelfinavir use during pregnancy
J. Med. Virol.
Soren Gantt and Erin Leister and Denise L. Jacobsen and Isabelle Boucoiran and Meei-Li Huang and Keith R. Jerome and Gonzague Jourdain and Nicole Ngo-Giang-Huong and Sandra Burchett and Lisa Frenkel
DOI: 10.1002/jmv.24420
11/2015

Nelfinavir Impairs Glycosylation of Herpes Simplex Virus 1 Envelope Proteins and Blocks Virus Maturation
Advances in Virology
Soren Gantt and Eliora Gachelet and Jacquelyn Carlsson and Serge Barcy and Corey Casper and Michael Lagunoff
DOI: 10.1155/2015/687162
2015

Development of Online Branched Learning Modules in Pediatric Transplant Infectious Diseases
Open Forum Infectious Diseases
Bernhard Wiedermann and Monica I. Ardura and Lara Danziger-Isakov and Soren Gantt and Blanca E Gonzalez and Tanvi Sharma
DOI: 10.1093/ofid/ofv133.314
2015

Bacillus Pumilus; A Rare Cause of Paediatric Septic Arthritis. A Case Report
JOURNAL OF RHEUMATOLOGY
2015

Management of influenza infection in children and pregnant women in BC, an update
382 the Mobile Medical Unit: A unique program in British columbia
2015

Myeloid Derived Suppressor Cells Are Present at High Frequency in Neonates and Suppress In Vitro T Cell Responses
PLoS ONE
Ana Gervassi and Nicholas Lejarcegui and Sandra Dross and Amanda Jacobson and Grace Itaya and Elvis Kidzeru and Soren Gantt and Heather Jaspan and Helen Horton
DOI: 10.1371/journal.pone.0107816
09/2014

The Role of Myeloid-Derived Suppressor Cells in Immune Ontogeny
Front. Immunol.
Soren Gantt and Ana Gervassi and Heather Jaspan and Helen Horton
DOI: 10.3389/fimmu.2014.00387
08/2014

Reduced human herpesvirus-8 oropharyngeal shedding associated with protease inhibitor-based antiretroviral therapy
Journal of Clinical Virology
Soren Gantt and Ashok Cattamanchi and Elizabeth Krantz and Amalia Magaret and Stacy Selke and Steven R. Kuntz and Meei-Li Huang and Lawrence Corey and Anna Wald and Corey Casper
DOI: 10.1016/j.jcv.2014.03.002
06/2014

Breast milk transmission of nevirapine-resistant HIV-1 to infants after single-dose nevirapine in Beira, Mozambique
Journal of Infectious Diseases
2014

Acute flaccid paralysis in a child infected with enterovirus D68: A case report
BC Medical Journal
2014

The Immunologic Basis for Severe Neonatal Herpes Disease and Potential Strategies for Therapeutic Intervention
DOI: 10.1155/2013/369172
2013

Early Life Immunity and Ability to Respond to Vaccines Hindered by Myeloid Derived Suppressor Cells: Implications for Infant HIV Vaccinology
AIDS RESEARCH AND HUMAN RETROVIRUSES
2013

Human herpesvirus 6, 7 and 8 in solid organ transplantation
American Journal of Transplantation
2013

Recommended Curriculum for Training in Pediatric Transplant Infectious Diseases
Journal of the Pediatric Infectious Diseases Society
L. Danziger-Isakov and U. Allen and J. Englund and B. Herold and J. Hoffman and M. Green and S. Gantt and D. Kumar and M. G. Michaels and on behalf of the American Society of Transplantation and Pediatric Infectious Diseases Society and and International Pediatric Transplant Association
DOI: 10.1093/jpids/pit079
2013

Implications of Age-Dependent Immune Responses to Enterovirus 71 Infection for Disease Pathogenesis and Vaccine Design
DOI: 10.1093/jpids/pit017
2013

Insights into the broad cellular effects of nelfinavir and the HIV protease inhibitors supporting their role in cancer treatment and prevention
DOI: 10.1097/cco.0b013e328363dfee
2013

An artesunate-containing antimalarial treatment regimen did not suppress cytomegalovirus viremia
DOI: 10.1016/j.jcv.2013.06.008
2013

Central Venous Catheter Repair Is Associated With an Increased Risk of Bacteremia and Central Line-associated Bloodstream Infection in Pediatric Patients
DOI: 10.1097/inf.0b013e31823eeec5
2012

Valganciclovir dosing using area under the curve calculations in pediatric solid organ transplant recipients
Pediatr Transplantation
David Villeneuve and Adam Brothers and Eric Harvey and Mariska Kemna and Yuk Law and Thomas Nemeth and Soren Gantt
DOI: 10.1111/petr.12030
2012

Nevirapine-Resistant HIV-1 DNA in Breast Milk After Single-Dose Nevirapine With or Without Zidovudine for Prevention of Mother-to-Child Transmission
DOI: 10.1093/jpids/pis065
2012

Effects of Short-Course Zidovudine on the Selection of Nevirapine-Resistant HIV-1 in Women Taking Single-Dose Nevirapine
DOI: 10.1093/infdis/jis282
2012

the roles of viral replication and antiviral treatment
DOI: 10.1097/qco.0b013e3283486d04
2011

Human herpesvirus 8-associated neoplasms: the roles of viral replication and antiviral treatment
Current opinion in infectious diseases
2011

The HIV Protease Inhibitor Nelfinavir Inhibits Kaposi's Sarcoma-Associated Herpesvirus Replication In Vitro
DOI: 10.1128/aac.01295-10
2011

Two paediatric cases of skin and soft-tissue infections due to clindamycin-resistant Staphylococcus aureus carrying a plasmid-encoded vga(A) allelic variant for a putative efflux pump
DOI: 10.1016/j.ijantimicag.2011.03.007
2011

Outcomes from pandemic influenza A H1N1 infection in recipients of solid-organ transplants: a multicentre cohort study
DOI: 10.1016/s1473-3099(10)70133-x
2010

Genetic Analyses of HIV-1 env Sequences Demonstrate Limited Compartmentalization in Breast Milk and Suggest Viral Replication within the Breast That Increases with Mastitis
DOI: 10.1128/jvi.00543-10
2010

Clinical presentation and outcome of epidemic Kaposi sarcoma in Ugandan children
DOI: 10.1186/1750-9378-5-s1-a12
2010

Nevirapine Resistance by Timing of HIV Type 1 Infection in Infants Treated with Single-Dose Nevirapine
DOI: 10.1086/652151
2010

Intermittent preventive treatment using artemisinin-based combination therapy reduces malaria morbidity among school-aged children in Mali
DOI: 10.1111/j.1365-3156.2009.02294.x
2009

Cytomegalovirus and Epstein-Barr virus in breast milk are associated with HIV-1 shedding but not with mastitis
DOI: 10.1097/qad.0b013e32830184f2
2008

Laboratory Indicators of Mastitis Are Not Associated with Elevated HIV-1 DNA Loads or Predictive of HIV-1 RNA Loads in Breast Milk
DOI: 10.1086/519843
2007

Use of 16S Ribosomal DNA Polymerase Chain Reaction to Identify Haemophilus influenzae Type b as the Etiology of Pericarditis in an Infant
The Pediatric Infectious Disease Journal
Crystal Benson and Soren Gantt and Danielle M Zerr and Xuan Qin and Patrick Abe
DOI: 10.1097/01.inf.0000154593.22356.e2
03/2005

Levels of circumsporozoite protein in the Plasmodium oocyst determine sporozoite morphology
The EMBO journal
2002

Antibodies against Thrombospondin-Related Anonymous Protein Do Not Inhibit Plasmodium Sporozoite Infectivity In Vivo
DOI: 10.1128/iai.68.6.3667-3673.2000
2000

Conservation of a Gliding Motility and Cell Invasion Machinery in Apicomplexan Parasites
J Cell Biol
Stefan Kappe and Thomas Bruderer and Soren Gantt and Hisashi Fujioka and Victor Nussenzweig and Robert Ménard
DOI: 10.1083/jcb.147.5.937
11/1999

Proteasome Inhibitors Block Development ofPlasmodium spp.
Antimicrobial agents and chemotherapy
1998

Cell Adhesion to a Motif Shared by the Malaria Circumsporozoite Protein and Thrombospondin Is Mediated by Its Glycosaminoglycan-binding Region and Not by CSVTCG
DOI: 10.1074/jbc.272.31.19205
1997

Research

Current Projects
Infections with human herpesviruses (HHVs), such as cytomegalovirus (CMV), Epstein-Barr virus (EBV), and Kaposi's sarcoma-associated herpesvirus (KSHV), cause an enormous amount of morbidity and mortality worldwide. Although nearly everyone becomes infected with one or more of these viruses, most have no significant illness from them. However, in young infants or people immunocompromised by HIV or having a transplant, these viruses frequently cause a range of severe infections and cancers. Vaccines or drugs to prevent these infections or their complications would have profound public health implications, but first additional research is required to better understand the underlying pathogenesis. As such, we carry out translational research studies, utilizing cohorts of children in Africa and North America as well as epidemiologic, clinical, and molecular approaches, to address fundamental questions about how to prevent and treat HHV-related diseases.

We are particularly interested in the role of HHV infections very early in life. For example, CMV infects approximately 1% of all infants in utero, which frequently results in neurologic sequelae like deafness and mental retardation; postnatal infections, however, are normally benign. Similarly, high risk for Burkitt's lymphoma, one of the most common childhood cancers in Africa, appears to be due to EBV infection in early infancy combined with exposure to malaria. We have established a cohort of children in Uganda who were followed every week during the first year of life to study CMV and EBV infection. The precise timing of infection with these viruses was established by weekly PCR testing. Comprehensive data were collected regarding epidemiologic and biologic risks of acquisition, including detailed behavioral questionnaires, determination of the viral exposure by household contacts, and collection of blood for measurement of virus-specific neutralizing maternal antibodies. Symptoms of illness associated with infection were captured using standardized physical exams and clinical histories, and blood was collected to evaluate the humoral and cellular immune responses to these viruses that are associated with control of viral replication.

Another recent focus of our group has been to elucidate the mechanism of action of the HIV protease inhibitor (PI) nelfinavir to inhibit HHV replication, and investigate the drug's potential to prevent and treat HHV-related diseases. We discovered that nelfinavir is able to block in vitro replication of all HHVs tested to date, at concentrations that are achieved in patients taking doses that are FDA-approved for treatment of HIV. This activity is unique to nelfinavir among PIs, and appears to result from effects of nelfinavir on host cell pathways that are required for viral replication, rather than on the virus itself. Interestingly, at least some of these cellular effects also confer nelfinavir's increasingly appreciated antitumor activities. Because nelfinavir is safe for use in humans, we have undertaken a phase 1/2 trial of nelfinavir for the treatment of Kaposi's sarcoma in Africa and the US. In addition, because nelfinavir is also safe during pregnancy, we are testing whether nelfinavir given to HIV-infected women during pregnancy can prevent congenital CMV infection.

Grants

CIHR Operating Grant - Project: "Correlates of acquisition and immune control of primary Epstein-Barr virus infection in Ugandan infants" – 2014-19

P01 Project: “Immune Control of KSHV Infection and Impact of HIV Infection And Therapy”

NIH (Co-PIs: Soren Gantt and Serge Barcy) 03/01/11 – 02/29/16

Honours & Awards

Sigma Xi Society, 1993

Honors for Undergraduate Research, Brown University, 1993

Rosenbluth Foundation Travel Award, 1999

Research Group Members

Jairah Alindogan, Direct study student
Catherine Byrne, PhD Student
April Christiansen, Research Trainee
Jason Jassal, Research Trainee
Aaron Liu, Doctoral Student
Rory Long, INSPIRE Student
Citlali Marquez, Postdoctoral fellow
Habibur Rahman
Shabnam Raufi, Research Volunteer
Carolyn Smith, Program Assistant
Guadalein Tanunliong, Directed study student
Jessica Tuengel, Graduate Research Asst
Emily Wong, Trainee