Overview

Flow cytometry is a technology that can be used to rapidly analyze physical and chemical properties of individual cells. It is a powerful tool with many applications in multiple disciplines such as blood cancers, immunology, molecular biology, and cellular therapy. For example, it is very effective for the study of the immune system and its response to infectious diseases and cancer.

Publications

Circulating rhabdoid tumor cells in the peripheral blood of a neonate
American Journal of Hematology
Maryam Al-Bakri and Jefferson Terry and Kate Chipperfield and Douglas Morrison and Audi Setiadi
DOI: 10.1002/ajh.26624
06/2022

Malignancy-associated haemophagocytic lymphohistiocytosis
The Lancet Haematology
Audi Setiadi and Adi Zoref-Lorenz and Christina Y Lee and Michael B Jordan and Luke Y C Chen
DOI: 10.1016/S2352-3026(21)00366-5
03/2022

Targeted treatment of immune thrombocytopenia in CTLA-4 insufficiency: a case report
British Journal of Haematology
Lai, C.M.B. and Setiadi, A. and Barlas, A. and Kanani, A. and Pourshahnazari, P. and Leitch, H.A. and Metzger, D.L. and Merkeley, H. and Biggs, C.M.
DOI: 10.1111/bjh.17866
2022

T-lymphoblastic transformation of chronic myeloid leukemia
eJHaem
Maryam Al Bakri and Audi Setiadi
DOI: 10.1002/jha2.125
02/2021

Clinical and laboratory features associated with myeloperoxidase expression in pediatric B-lymphoblastic leukemia
Cytometry Part B - Clinical Cytometry
McGinnis, E. and Yang, D. and Au, N. and Morrison, D. and Chipperfield, K.M. and Setiadi, A.F. and Liu, L. and Tsang, A. and Vercauteren, S.M.
DOI: 10.1002/cyto.b.21966
2021

EDTA-induced platelet hypogranulation: beware of dysplasia mimic!
Journal of Hematopathology
Al Moosawi, M. and Vawda, A. and Setiadi, A.
DOI: 10.1007/s12308-021-00445-9
2021

CD138-negative plasma cell myeloma: a diagnostic challenge and a unique entity.
BMJ case reports
DOI: 10.1136/bcr-2019-232233
PubMed: 31791993
12/2019

The significance of peripheral blood minimal residual disease to predict early disease response in patients with B-cell acute lymphoblastic leukemia
International Journal of Laboratory Hematology
A. Setiadi and D. Owen and A. Tsang and R. Milner and S. Vercauteren
DOI: 10.1111/ijlh.12535
10/2016

Notch initiates the endothelial-to-mesenchymal transition in the atrioventricular canal through autocrine activation of soluble guanylyl cyclase.
Developmental cell
DOI: 10.1016/j.devcel.2011.06.022
PubMed: 21839921
08/2011

Differential regulation of transforming growth factor beta signaling pathways by Notch in human endothelial cells.
The Journal of biological chemistry
DOI: 10.1074/jbc.m109.011833
PubMed: 19473993
05/2009

Differential regulation of transforming growth factor ß signaling pathways by notch in human endothelial cells
Journal of Biological Chemistry
Fu, Y.X. and Chang, A. and Chang, L. and Niessen, K. and Eapen, S. and Setiadi, A. and Karsan, A.
DOI: 10.1074/jbc.M109.011833
2009

Research

Minimal Residual Disease & Leukemic Stem Cells
Minimal residual disease refers to a small number of malignant cells that remain in the patient during or after treatment when the patient has no signs or symptoms of the disease. Flow cytometry allows for MRD analysis by looking at different expression of cellular antigens on malignant cells compared to normal cells. As part of Dr. Suzanne Vercauteren’s team, our lab has implemented 10-color flow cytometry panels for MRD detection in B-cell acute lymphoblastic leukemia (the most common type of blood cancer in children) post chemotherapy and post CD19 directed therapy. In collaboration with the BRAVe initiative, we are currently looking at more markers to identify leukemic stem cell populations, which may contribute to relapse or treatment resistance.

Primary Immunodeficiency Disorders and Hemophagocytic Lymphohistiocytosis
PIDDs are a heterogeneous group of inherited disorders resulting from genetic defects that impair the immune system. Given the complexity of the immune system, the clinical manifestations of PIDDs are highly variable and may present with recurrent infections, autoimmunity and predisposition to cancer. Under the “Diseases of Immune Dysregulation” group of PIDD is a life threatening disorder called Hemophagocytic Lymphohistiocytosis (HLH), leading to an overactive, dysfunctional response of immune system, low blood cell counts and multi-organ damage. Our lab implemented clinical flow cytometry panels to support newborn screening for severe combined immunodeficiency (SCID) and expand the investigation for PIDD, as well as Perforin assay for screening of primary HLH. We continue to optimize our assay methodologies and reference ranges to support the diagnosis of PIDD in BC.

Cellular Therapy
Our lab is validating several flow cytometry panels for product assessment of several graft engineering projects under TRACE initiative, including T-cell alpha/beta depleted stem cell transplantation to reduce GvHD risk, as well as viral specific T-cell therapy for severe protracted viral infection post-transplant.

Research Group Members

Michelle Dittrick, Clinical Research Program Manager
Lorraine Liu, Clinical flow core manager