- Overview
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My focus is on doing more with less. If we need to take blood from a child, then take as little as possible. If we need to take a biopsy, can we get both diagnosis and a treatment plan all at once? Combining what we know from many years of research with what we want (accurate diagnosis, treatment options) helps personalize drug treatment regimens and minimize blood collections, all while improving the overall patient experience and providing better outcomes. By working with cutting edge technologies and utilizing the extensive expertise of physicians, pathologists and scientists, we can drive substantial improvement in patient health and wellbeing.
- Publications
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Is It Time to Retire Aldosterone Suppression Testing?
American Journal of Hypertension
Gregory A Kline and Alexander A Leung and Dennis Orton and James MacFarlane and Mark Gurnell
DOI: 10.1093/ajh/hpaf163
08/2025Big data determination and validation of reference range for 24-h urine cortisol by liquid chromatography-mass spectrometry
European Journal of Endocrinology
Kline, G.A. and Venos, E.S. and Campbell, D. and Leung, A.A. and Orton, D.
DOI: 10.1093/ejendo/lvaf054
2025Nociceptor neurons suppress alveolar macrophage-induced Siglec-F+ neutrophil-mediated inflammation to protect against pulmonary fibrosis
Immunity
Hiroki, C.H. and Hassanabad, M.F. and Defaye, M. and Sarden, N. and Bartlett, A. and Farias, R. and Nguyen, A.P. and Guerrero-Fonseca, I.M. and Yoon, G. and Brown, L. and Ma, C. and Yang, H. and Belke, D. and Hassanabad, A.F. and McCoy, C. and Rosin, N.L. and Orton, D.J. and Fedak, P.W.M. and Vallance, B.A. and Yu, H. and Jacobson, K. and Khan, N. and Altier, C. and Kelly, M.M. and Yipp, B.G.
DOI: 10.1016/j.immuni.2025.05.002
2025Outcomes After Unilateral Adrenalectomy in Asymmetrical Bilateral Primary Aldosteronism
Hypertension
Grundy, M. and Leung, A.A. and Pasieka, J.L. and Harvey, A. and So, B. and Caughlin, C. and Przybojewski, S. and Orton, D. and Hyrcza, M. and Kline, G.A.
DOI: 10.1161/HYPERTENSIONAHA.125.24849
2025Advancing Newborn Screening in Washington State: A Novel Multiplexed LC-MS/MS Proteomic Assay for Wilson Disease and Inborn Errors of Immunity
International Journal of Neonatal Screening
Klippel, C. and Park, J. and Sandin, S. and Winstone, T.M.L. and Chen, X. and Orton, D. and Singh, A. and Hill, J.D. and Shahbal, T.K. and Hamacher, E. and Officer, B. and Thompson, J. and Duong, P. and Grotzer, T. and Hahn, S.H.
DOI: 10.3390/ijns11010006
2025Discordance between adrenal vein sampling and imaging: the role of unilateral cortisol excess
European Journal of Endocrinology
Grundy, M. and Leung, A.A. and Hyrcza, M. and Pasieka, J.L. and Chan, D. and Przybojewski, S.J. and So, C.-B.B. and Orton, D. and Harvey, A. and Kline, G.A.
DOI: 10.1093/ejendo/lvaf019
2025Confirmatory Testing for Primary Aldosteronism A Study of Diagnostic Test Accuracy
Annals of Internal Medicine
Leung, A.A. and Padwal, R.S. and Hundemer, G.L. and Venos, E. and Campbell, D.J.T. and Holmes, D.T. and Orton, D.J. and So, C.B. and Przybojewski, S.J. and Caughlin, C.E. and Pasieka, J.L. and Rabi, D.M. and Kline, G.A.
DOI: 10.7326/ANNALS-24-03153
2025Targeted proteomic approach for quantification of collagen type I and type III in formalin-fixed paraffin-embedded tissue
Scientific Reports
Nicole L. Rosin and Tara M. L. Winstone and Margaret Kelley and Jeff Biernaskie and Antoine Dufour and Dennis J. Orton
DOI: 10.1038/s41598-024-68377-9
08/2024Understanding Elevated Vitamin D Measurements to Uncover Hypercalcemia Etiology
Clinical Chemistry
Sajid, U. and Orton, D. and Kaufmann, M. and Jones, G. and Kline, G.A.
DOI: 10.1093/clinchem/hvae044
2024Increased Lp(a) Workload Volumes Reflect Uptake of the 2021 Canadian Cardiovascular Society Guidelines for Management of Dyslipidemia
Canadian Journal of Cardiology
Orton, D.J. and Gifford, J.L. and Raizman, J.E. and Pearson, G.J. and Tsui, A.K.Y.
DOI: 10.1016/j.cjca.2024.02.019
2024Distribution of videos demonstrating best practices in preventing hemolysis is associated with reduced hemolysis among nurse-collected specimens in hospitals
Clinical Biochemistry
Lawrence de Koning and Dennis Orton and Isolde Seiden Long and Jessica Boyd and Mark Kellogg and Amid Abdullah and Christopher Naugler and Albert Tsui and Brenda Strange and Dory Glaser
DOI: 10.1016/j.clinbiochem.2023.110632
09/2023Agreement of LC-MS assays for IGF-1 traceable to NIST and WHO standards permits harmonization of reference intervals between laboratories
Clinical Biochemistry
Sally Ezra and Tara M.L. Winstone and R. Singh and Dennis J. Orton
DOI: 10.1016/j.clinbiochem.2023.04.002
06/2023Biological variation in clozapine and metabolite reporting during therapeutic drug monitoring
Clinica Chimica Acta
Wickramarachchi, P. and Boyd, J.M. and Orton, D.J.
DOI: 10.1016/j.cca.2022.04.005
2022Exosomal proteomic analysis reveals changes in the urinary proteome of rats with unilateral ureteral obstruction
Canadian Journal of Chemistry
Orton, D.J. and Doucette, A.A. and Huang, W.-Y. and MacLellan, D.L.
DOI: 10.1139/cjc-2017-0681
2018Bone metabolism
Endocrine Biomarkers: Clinicians and Clinical Chemists in Partnership
Kline, G. and Orton, D. and Sadrzadeh, H.
DOI: 10.1016/B978-0-12-803412-5.00004-5
2017Novel approach to establishing an aldosterone: Renin ratio cutoff for primary aldosteronism
Hypertension
Leung, A.A. and Orton, D.J. and Chin, A. and Sadrzadeh, H. and Kline, G.A.
DOI: 10.1161/HYPERTENSIONAHA.116.08407
2017One-step extraction and quantitation of toxic alcohols and ethylene glycol in plasma by capillary gas chromatography (GC) with flame ionization detection (FID)
Clinical Biochemistry
Orton, D.J. and Boyd, J.M. and Affleck, D. and Duce, D. and Walsh, W. and Seiden-Long, I.
DOI: 10.1016/j.clinbiochem.2015.09.007
2016Fasting time and vitamin B12 levels in a community-based population
Clinica Chimica Acta
Orton, D.J. and Naugler, C. and Sadrzadeh, S.M.H.
DOI: 10.1016/j.cca.2016.05.005
2016Critically high plasma ammonia in an adolescent girl
Clinical Chemistry
Orton, D.J. and Gifford, J.L. and Seiden-Long, I. and Khan, A. and De Koning, L.
DOI: 10.1373/clinchem.2016.259473
2016Resolubilization of precipitated intact membrane proteins with cold formic acid for analysis by mass spectrometry
Journal of Proteome Research
Doucette, A.A. and Vieira, D.B. and Orton, D.J. and Wall, M.J.
DOI: 10.1021/pr500864a
2014Reprint of "GELFrEE fractionation combined with mass spectrometry for proteome analysis of secreted toxins from Enteropathogenic Escherichia coli (EPEC)"
Molecular and Cellular Probes
Orton, D.J. and Arsenault, D.J. and Thomas, N.A. and Doucette, A.A.
DOI: 10.1016/j.mcp.2014.01.004
2014Proteomic analysis of rat proximal tubule cells following stretch-induced apoptosis in an in vitro model of kidney obstruction
Journal of Proteomics
Orton, D.J. and Doucette, A.A. and Maksym, G.N. and MacLellan, D.L.
DOI: 10.1016/j.jprot.2013.11.025
2014Dual LC-MS platform for high-throughput proteome analysis
Journal of Proteome Research
Orton, D.J. and Wall, M.J. and Doucette, A.A.
DOI: 10.1021/pr400738a
2013GELFrEE fractionation combined with mass spectrometry for proteome analysis of secreted toxins from Enteropathogenic Escherichia coli (EPEC)
Molecular and Cellular Probes
Orton, D.J. and Arsenault, D.J. and Thomas, N.A. and Doucette, A.A.
DOI: 10.1016/j.mcp.2013.06.004
2013Proteomic workflows for biomarker identification using mass spectrometry - Technical and statistical considerations during initial discovery
Proteomes
Orton, D.J. and Doucette, A.A.
DOI: 10.3390/proteomes1020109
2013A universal, high recovery assay for protein quantitation through temperature programmed liquid chromatography (TPLC)
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Orton, D.J. and Doucette, A.A.
DOI: 10.1016/j.jchromb.2013.01.021
2013The htpAB operon of Legionella pneumophila cannot be deleted in the presence of the groE chaperonin operon of Escherichia coli
Canadian Journal of Microbiology
Nasrallah, G.K. and Gagnon, E. and Orton, D.J. and Garduño, R.A.
DOI: 10.1139/w11-086
2011 - Research
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Targeted proteomic approach for quantification of collagen subtypes in formalin-fixed paraffin-embedded tissue
This project was established to quantify specific proteins in FFPE tissue. Here we develop our understanding of extracellular matrix protein composition during wound healing and fibrosis. The project was a proof-of-principle application to build targeted protein quantitation methods from FFPE tissues and may be applied to a variety of targets.Development and evaluation of a NUDT15 phenotyping assay in blood
NUDT15 is an enzyme in the metabolism of thiopurine medications that when deficient, causes significant adverse drug reactions to these medications. Primarily occurring in Asian populations, there are no current methods available to phenotype this enzyme and genotyping is often limited to analyzing a single polymorphism while dozens have been described.Development of a screening method to predict adverse outcomes in 5-FU chemotherapy
5-Fluorouracil is a chemotherapeutic drug with significant associated toxicity. Similar to the above NUDT15 polymorphism, this project was developed to help predict which patients, if any, may experience toxicity associated with a metabolic enzyme deficiency (DPYD).GrantsIGF-1 in Treatment of Growth Hormone Deficiency. Funding Source: Pfizer Long-Acting Growth Hormone Research
Congratulations to the winners of the Nobel Prize in Physiology or Medicine 2025
The Nobel Prize in Physiology or Medicine 2025 was awarded to Dr. Mary E. Brunkow, Dr. Fred Ramsdell, and Dr. Shimon Sakaguchi for their discoveries concerning peripheral immune tolerance. BCCHR's Dr. Megan Levings reflects on the significance of this area of research and how these immune cells could develop treatments for numerous diseases such as type 1 diabetes, multiple sclerosis, organ rejection, and more.
