- Overview
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My research team strives to develop new diagnostic and therapeutic approaches to detect and treat children suffering from cancer earlier, better and with reduced impact on their life.
The fundamental question is how cancer cells are different from healthy, normal cells? If we understand this we will be able to better detect and kill cancer while leaving the rest of the body untouched.
Our research focuses on proteins, the structural and functional building blocks of a cell. To do this we combine genomics and proteomics, a technology that enables us to monitor all of the proteins in our body and detect how they are changed in childhood cancer. We then use computational approaches to further analyze and integrate our findings and to make them accessible to clinicians and fellow scientists around the world.
- Publications
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RNA Polymerase II transcription independent of TBP in murine embryonic stem cells
eLife
James ZJ Kwan and Thomas F Nguyen and Anuli C Uzozie and Marek A Budzynski and Jieying Cui and Joseph MC Lee and Filip Van Petegem and Philipp F Lange and Sheila S Teves
DOI: 10.7554/eLife.83810
03/2023ASAP-Automated Sonication-Free Acid-Assisted Proteomes-from Cells and FFPE Tissues
Analytical Chemistry
Georgina D. Barnabas and Verena Goebeler and Janice Tsui and Jonathan W. Bush and Philipp F. Lange
DOI: 10.1021/acs.analchem.2c04264
02/2023SQuAPP—simple quantitative analysis of proteins and PTMs
Bioinformatics
Enes K Ergin and Anuli C Uzozie and Siyuan Chen and Ye Su and Philipp F Lange
DOI: 10.1093/bioinformatics/btac628
10/2022Multi-omic profiling of the leukemic microenvironment shows bone marrow interstitial fluid is distinct from peripheral blood plasma
Lorenz Nierves and Jian Guo and Siyuan Chen and Janice Tsui and Anuli Uzozie and Jonathan W. Bush and Tao Huan and Philipp F. Lange
DOI: 10.1101/2022.03.30.486272
04/2022Pathogenic BRCA1 variants disrupt PLK1-regulation of mitotic spindle orientation
Nature Communications
Zhengcheng He and Ryan Ghorayeb and Susanna Tan and Ke Chen and Amanda C. Lorentzian and Jack Bottyan and Syed Mohammed Musheer Aalam and Miguel Angel Pujana and Philipp F. Lange and Nagarajan Kannan and Connie J. Eaves and Christopher A. Maxwell
DOI: 10.1038/s41467-022-29885-2
04/2022Persistence of targetable lesions, predicted therapy sensitivity and proteomes through disease evolution in pediatric acute lymphoblastic leukemia
Amanda C. Lorentzian and Jenna Rever and Enes K. Ergin and Meiyun Guo and Neha M. Akella and Nina Rolf and C. James Lim and Gregor S.D. Reid and Christopher A. Maxwell and Philipp F. Lange
DOI: 10.1101/2022.03.04.22271927
03/2022Sensitive Identification of Known and Unknown Protease Activities by Unsupervised Linear Motif Deconvolution
Analytical Chemistry
Anuli C. Uzozie and Theodore G. Smith and Siyuan Chen and Philipp F. Lange
DOI: 10.1021/acs.analchem.1c04937
02/2022A cross-standardized flow cytometry platform to assess phenotypic stability in precursor B-cell acute lymphoblastic leukemia (B-ALL) xenografts
Cytometry Part A
Nina Rolf and Lorraine Y. T. Liu and Angela Tsang and Philipp F. Lange and Chinten James Lim and Christopher A. Maxwell and Suzanne M. Vercauteren and Gregor S. D. Reid
DOI: 10.1002/cyto.a.24473
01/2022Shift of the insoluble content of the proteome in aging mouse brain
bioRxiv
Molzahn, C. and Kuechler, E. and Zemlyankina, I. and Nieves, L. and Ali, T. and Cole, G. and Wang, J. and Albu, R.F. and Zhu, M. and Cashman, N. and Gilch, S. and Karsan, A. and Lange, P.F. and Gsponer, J. and Mayor, T.
DOI: 10.1101/2022.12.13.520290
2022HUNTER: Sensitive Automated Characterization of Proteolytic Systems by N Termini Enrichment from Microscale Specimen
Methods in Molecular Biology
Uzozie, A.C. and Tsui, J. and Lange, P.F.
DOI: 10.1007/978-1-0716-2124-0_8
2022Isolation of Detergent Insoluble Proteins from Mouse Brain Tissue for Quantitative Analysis Using Data Independent Acquisition (DIA)
Methods in Molecular Biology
Molzahn, C. and Nierves, L. and Lange, P.F. and Mayor, T.
DOI: 10.1007/978-1-0716-2124-0_3
2022Multi-omic profiling of the leukemic microenvironment shows bone marrow interstitial fluid is distinct from peripheral blood plasma
Experimental Hematology and Oncology
Nierves, L. and Guo, J. and Chen, S. and Tsui, J. and Uzozie, A.C. and Bush, J.W. and Huan, T. and Lange, P.F.
DOI: 10.1186/s40164-022-00310-0
2022Modification of BRCA1-associated breast cancer risk by HMMR overexpression
Nature Communications
Mateo, F. and He, Z. and Mei, L. and de Garibay, G.R. and Herranz, C. and García, N. and Lorentzian, A. and Baiges, A. and Blommaert, E. and Gómez, A. and Mirallas, O. and Garrido-Utrilla, A. and Palomero, L. and Espín, R. and Extremera, A.I. and Soler-Monsó, M.T. and Petit, A. and Li, R. and Brunet, J. and Chen, K. and Tan, S. and Eaves, C.J. and McCloskey, C. and Hakem, R. and Khokha, R. and Lange, P.F. and Lázaro, C. and Maxwell, C.A. and Pujana, M.A.
DOI: 10.1038/s41467-022-29335-z
2022Sensitive identification of known and unknown protease activities by unsupervised linear motif deconvolution
Anuli C. Uzozie and Theodore G. Smith and Siyuan Chen and Philipp F. Lange
DOI: 10.1101/2021.11.15.468703
11/2021Detectability of Biotin Tags by LC–MS/MS
Journal of Proteome Research
Lorenz Nierves and Philipp F. Lange
DOI: 10.1021/acs.jproteome.0c01049
05/2021Robust unsupervised deconvolution of linear motifs characterizes 68 protein modifications at proteome scale
Scientific Reports
Smith, T.G. and Uzozie, A.C. and Chen, S. and Lange, P.F.
DOI: 10.1038/s41598-021-01971-3
2021PDX models reflect the proteome landscape of pediatric acute lymphoblastic leukemia but divert in select pathways
Journal of Experimental and Clinical Cancer Research
Uzozie, A.C. and Ergin, E.K. and Rolf, N. and Tsui, J. and Lorentzian, A. and Weng, S.S.H. and Nierves, L. and Smith, T.G. and Lim, C.J. and Maxwell, C.A. and Reid, G.S.D. and Lange, P.F.
DOI: 10.1186/s13046-021-01835-8
2021Detectability of biotin tags by LC-MS/MS
Lorenz Nierves and Philipp F. Lange
DOI: 10.1101/2020.12.30.424786
12/2020Multi-Omic Approach to Identify Phenotypic Modifiers Underlying Cerebral Demyelination in X-Linked Adrenoleukodystrophy.
Frontiers in cell and developmental biology
Richmond PA and van der Kloet F and Vaz FM and Lin D and Uzozie A and Graham E and Kobor M and Mostafavi S and Moerland PD and Lange PF and van Kampen AHC and Wasserman WW and van Karnebeek CDM
DOI: 10.3389/fcell.2020.00520
PubMed: 32671069
06/2020Fold-Change Compression: An Unexplored But Correctable Quantitative Bias Caused by Nonlinear Electrospray Ionization Responses in Untargeted Metabolomics.
Analytical chemistry
Yu H and Xing S and Nierves L and Lange PF and Huan T
DOI: 10.1021/acs.analchem.0c00246
PubMed: 32319750
05/2020Multi-omic approach to identify phenotypic modifiers underlying cerebral demyelination in X-linked adrenoleukodystrophy
Richmond PA and van der Kloet F and Vaz FM and Lin D and Uzozie A and Graham EJ and Kobor MS and Mostafavi S and Moerland PD and Lange PF and van Kampen AHC and Wasserman WW and van Karnebeek C
DOI: 10.1101/2020.03.19.20035063
03/2020Sensitive Determination of Proteolytic Proteoforms in Limited Microscale Proteome Samples
Molecular & Cellular Proteomics
Samuel S.H. Weng and Fatih Demir and Enes K. Ergin and Sabrina Dirnberger and Anuli Uzozie and Domenic Tuscher and Lorenz Nierves and Janice Tsui and Pitter F. Huesgen and Philipp F. Lange
DOI: 10.1074/mcp.TIR119.001560
11/2019Sensitive determination of proteolytic proteoforms in limited microscale proteome samples
Samuel S.H. Weng and Fatih Demir and Enes K. Ergin and Sabrina Dirnberger and Anuli Uzozie and Domenic Tuscher and Lorenz Nierves and Janice Tsui and Pitter F. Huesgen and Philipp F. Lange
DOI: 10.1101/566109
03/2019Origins and clinical relevance of proteoforms in pediatric malignancies
Expert Review of Proteomics
Amanda Lorentzian and Anuli Uzozie and Philipp F. Lange
DOI: 10.1080/14789450.2019.1575206
02/2019Tumor Variant Identification That Accounts for the Unique Molecular Landscape of Pediatric Malignancies
JNCI Cancer Spectrum
Amanda Lorentzian and Jaclyn A Biegel and D Gigi Ostrow and Nina Rolf and Chi-Chao Liu and S Rod Rassekh and Rebecca J Deyell and Timothy Triche and Kirk R Schultz and Jacob Rozmus and Gregor S D Reid and C James Lim and Philipp F Lange and Christopher A Maxwell
DOI: 10.1093/jncics/pky079
01/2019PDX models reflect the proteome landscape of pediatric acute lymphoblastic leukemia but divert in select pathways
bioRxiv
Uzozie, A.C. and Ergin, E.K. and Rolf, N. and Tsui, J. and Lorentzian, A. and Weng, S.S. and Nierves, L. and Smith, T.G. and James Lim, C. and Maxwell, C.A. and Reid, G.S.D. and Lange, P.F.
DOI: 10.1101/2019.12.18.880401
2019Tumor Variant Identification That Accounts for the Unique Molecular Landscape of Pediatric Malignancies
JNCI Cancer Spectrum
Lorentzian, A. and Biegel, J.A. and Ostrow, D.G. and Rolf, N. and Liu, C.-C. and Rassekh, S.R. and Deyell, R.J. and Triche, T. and Schultz, K.R. and Rozmus, J. and Reid, G.S.D. and Lim, C.J. and Lange, P.F. and Maxwell, C.A.
DOI: 10.1093/JNCICS/PKY079
2018HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development
eLife
Marisa Connell and Helen Chen and Jihong Jiang and Chia-Wei Kuan and Abbas Fotovati and Tony LH Chu and Zhengcheng He and Tess C Lengyell and Huaibiao Li and Torsten Kroll and Amanda M Li and Daniel Goldowitz and Lucien Frappart and Aspasia Ploubidou and Millan S Patel and Linda M Pilarski and Elizabeth M Simpson and Philipp F Lange and Douglas W Allan and Christopher A Maxwell
DOI: 10.7554/elife.28672
10/2017HMMR acts in the PLK1-dependent spindle positioning pathway and supports neural development
eLife
Connell, M. and Chen, H. and Jiang, J. and Kuan, C.-W. and Fotovati, A. and Chu, T.L.H. and He, Z. and Lengyell, T.C. and Li, H. and Kroll, T. and Li, A.M. and Goldowitz, D. and Frappart, L. and Ploubidou, A. and Patel, M.S. and Pilarski, L.M. and Simpson, E.M. and Lange, P.F. and Allan, D.W. and Maxwell, C.A.
DOI: 10.7554/eLife.28672
2017Active site specificity profiling of the matrix metalloproteinase family: Proteomic identification of 4300 cleavage sites by nine MMPs explored with structural and synthetic peptide cleavage analyses
Matrix Biology
Eckhard, Ulrich and Huesgen, Pitter F. and Schilling, Oliver and Bellac, Caroline L. and Butler, Georgina S. and Cox, Jennifer H. and Dufour, Antoine and Goebeler, Verena and Kappelhoff, Reinhild and Keller, Ulrich Auf Dem and Klein, Theo and Lange, Philipp F. and Marino, Giada and Morrison, Charlotte J. and Prudova, Anna and Rodriguez, David and Starr, Amanda E. and Wang, Yili and Overall, Christopher M.
DOI: 10.1016/j.matbio.2015.09.003
2016TAILS N-Terminomics and Proteomics Show Protein Degradation Dominates over Proteolytic Processing by Cathepsins in Pancreatic Tumors
Cell Reports
Prudova, A. and Gocheva, V. and auf dem Keller, U. and Eckhard, U. and Olson, O.C. and Akkari, L. and Butler, G.S. and Fortelny, N. and Lange, P.F. and Mark, J.C. and Joyce, J.A. and Overall, C.M.
DOI: 10.1016/j.celrep.2016.06.086
2016Active site specificity profiling datasets of matrix metalloproteinases (MMPs) 1, 2, 3, 7, 8, 9, 12, 13 and 14
Data in Brief
Eckhard, U. and Huesgen, P.F. and Schilling, O. and Bellac, C.L. and Butler, G.S. and Cox, J.H. and Dufour, A. and Goebeler, V. and Kappelhoff, R. and auf dem Keller, U. and Klein, T. and Lange, P.F. and Marino, G. and Morrison, C.J. and Prudova, A. and Rodriguez, D. and Starr, A.E. and Wang, Y. and Overall, C.M.
DOI: 10.1016/j.dib.2016.02.036
2016COSMC knockdown mediated aberrant O-glycosylation promotes oncogenic properties in pancreatic cancer
Molecular Cancer
Hofmann, B.T. and Schlüter, L. and Lange, P. and Mercanoglu, B. and Ewald, F. and Fölster, A. and Picksak, A.-S. and Harder, S. and El Gammal, A.T. and Grupp, K. and Güngör, C. and Drenckhan, A. and Schlüter, H. and Wagener, C. and Izbicki, J.R. and Jücker, M. and Bockhorn, M. and Wolters-Eisfeld, G.
DOI: 10.1186/s12943-015-0386-1
2015Proteome TopFIND 3.0 with TopFINDer and PathFINDer: Database and analysis tools for the association of protein termini to pre- and post-translational events
Nucleic Acids Research
Fortelny, N. and Yang, S. and Pavlidis, P. and Lange, P.F. and Overall, C.M.
DOI: 10.1093/nar/gku1012
2015Active Site Specificity Profiling of the Matrix Metalloproteinase Family: Proteomic Identification of 4,300 Cleavage Sites by MMPs 1, 2, 3, 7, 8, 9, 12, 13, and 14.
2015LysargiNase mirrors trypsin for protein C-terminal and methylation-site identification
Nature Methods
Pitter F Huesgen and Philipp F Lange and Lindsay D Rogers and Nestor Solis and Ulrich Eckhard and Oded Kleifeld and Theodoros Goulas and F Xavier Gomis-Rüth and Christopher M Overall
DOI: 10.1038/nmeth.3177
11/2014Ensembles of protein termini and specific proteolytic signatures as candidate biomarkers of disease.
Huesgen PF and Lange PF and Overall CM
DOI: 10.1002/prca.201300104
PubMed: 24497460
02/2014Annotating N Termini for the Human Proteome Project: N Termini and N alpha-Acetylation Status Differentiate Stable Cleaved Protein Species from Degradation Remnants in the Human Erythrocyte Proteome
Journal of Proteome Research
Lange, Philipp F. and Huesgen, Pitter F. and Nguyen, Karen and Overall, Christopher M.
DOI: 10.1021/pr401191w
2014Macrophage matrix metalloproteinase-12 dampens inflammation and neutrophil influx in arthritis
Cell Reports
Bellac, C.L. and Dufour, A. and Krisinger, M.J. and Loonchanta, A. and Starr, A.E. and auf dem Keller, U. and Lange, P.F. and Goebeler, V. and Kappelhoff, R. and Butler, G.S. and Burtnick, L.D. and Conway, E.M. and Roberts, C.R. and Overall, C.M.
DOI: 10.1016/j.celrep.2014.09.006
2014Network Analyses Reveal Pervasive Functional Regulation Between Proteases in the Human Protease Web
PLoS Biology
Fortelny, N. and Cox, J.H. and Kappelhoff, R. and Starr, A.E. and Lange, P.F. and Pavlidis, P. and Overall, C.M.
DOI: 10.1371/journal.pbio.1001869
2014Characterization of LysargiNase for use in phosphoproteomics experiments, partII
2014LysargiNase and tryptic digest of MDA-MB 231 cell lysates
2014Protein TAILS: when termini tell tales of proteolysis and function.
Lange PF and Overall CM
DOI: 10.1016/j.cbpa.2012.11.025
PubMed: 23298954
02/2013Proteomic amino-termini profiling reveals targeting information for protein import into complex plastids.
Huesgen PF and Alami M and Lange PF and Foster LJ and Schröder WP and Overall CM and Green BR
DOI: 10.1371/journal.pone.0074483
PubMed: 24066144
2013TopFIND 2.0-linking protein termini with proteolytic processing and modifications altering protein function
Nucleic Acids Research
Lange, Philipp F. and Huesgen, Pitter F. and Overall, Christopher M.
DOI: 10.1093/nar/gkr1025
2012TopFIND, a knowledgebase linking protein termini with function
Nature Methods
Lange, Philipp F. and Overall, Christopher M.
DOI: 10.1038/nmeth.1669
2011Towards kit-like 18F-labeling of marimastat, a noncovalent inhibitor drug for in vivo PET imaging cancer associated matrix metalloproteases
MedChemComm
Li, Y. and Ting, R. and Harwig, C.W. and Auf Dem Keller, U. and Bellac, C.L. and Lange, P.F. and Inkster, J.A.H. and Schaffer, P. and Adam, M.J. and Ruth, T.J. and Overall, C.M. and Perrin, D.M.
DOI: 10.1039/c1md00117e
2011Novel Matrix Metalloproteinase Inhibitor [(18)F]Marimastat-Aryltrifluoroborate as a Probe for In vivo Positron Emission Tomography Imaging in Cancer
Cancer Research
Keller, Ulrich Auf Dem and Bellac, Caroline L. and Li, Ying and Lou, Yuanmei and Lange, Philipp F. and Ting, Richard and Harwig, Curtis and Kappelhoff, Reinhild and Dedhar, Shoukat and Adam, Michael J. and Ruth, Thomas J. and Benard, Francois and Perrin, David M. and Overall, Christopher M.
DOI: 10.1158/0008-5472.CAN-10-1584
2010ClC-7 requires Ostm1 as a beta-subunit to support bone resorption and lysosomal function
GBM Annual Spring meeting Mosbach 2007
Jens C. Fuhrmann and Philipp F. Lange and Lena Wartosch and Thomas J. Jentsch
DOI: 10.1240/sav_gbm_2007_m_001701
03/2007ClC-7 requires Ostm1 as a beta-subunit to support bone resorption and lysosomal function
Nature
Lange, PF and Wartosch, L and Jentsch, TJ and Fuhrmann, JC
DOI: 10.1038/nature04535
2006 - Research
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Current Projects
The overall objective of my team's research is to monitor and detect aberrant protein presence and function in cancer and exploit this difference to diagnose and treat cancer and improve the wellbeing of cancer survivors.
We are particularly interested in how the post-translational modification of proteins affects cancer progression and secondary disease, which can hit childhood cancer survivors years after successful treatment. After translation proteins can be modified by, for example, proteolytic processing or phosphorylation. This creates a repertoire of "proteoforms", which are all the variant and modified protein products of a single gene. They often differ in their localization, function and interaction with other proteins.
Our research program follows four main axes that inform each other:
I: We use proteo-genomics to study differences in tissue specimen from children suffering from select cancers and healthy individuals, and translate these findings into a new diagnostic and treatment approach.
II: We integrate cell biology, biochemistry, genomics, proteomics and bioinformatics to study the role of post-translational modification in the regulation of select cell signaling networks. By investigating patient-derived cells and mouse models we strive to identify new ways of altering these signaling networks to improve drug efficacy and reduce side effects.
III: Computational biology plays an integral role in our research. Our main focus is the development of algorithms for multi-scale data integraction, protein function analysis, pattern recognition and de-convolution of network effects in complex systems. Building on these we develop new biological knowledgebases and applications to improve the functional analysis of genomics and proteomics data and to guide personalized treatment decisions.
IV: We continuously strive to improve existing- and develop new technologies for the mass spectrometric analysis of complex biological specimen enabling a more comprehensive, specific and sensitive investigation of smaller biopsies.
My research team is embedded in the Michael Cuccione Childhood Cancer Research Program and affiliated with the BC Cancer Research Centre and BC Children’s Hospital. This creates a stimulating environment in which we work closely with world-class experts in clinical oncology, cancer biology, genomics and computer sciences.Grants"Proteolytic protein termini as a new strategy for cancer cell-specific therapy," Innovation Grant, Canadian Cancer Society, 2016-2018
"Bioinformatics resources for genomewide assessment of protein function at the proteoform level," NSERC Discovery Grant, $28,000/ year, 2018-2023
Honours & AwardsMichael Smith Health Foundation Research Scholar Award (2016)
Canada Research Chair Tier 2 Award in Translational Proteomics of Childhood Malignancies (2015-2020)
Feodor Lynen Research Fellowship for Postdoctoral Research (Alexander von Humboldt Foundation) (2009-2011)
Joint Michael Smith Foundation for Health Research & Breast Cancer Society of Canada Research Award (2011-2012)
Research Group MembersGeorgina Barnabas, Postdoctoral Fellow
Enes Kemal Ergin, Graduate Student
Lina Jenane, Graduate Student
Lorenz Nierves, PhD Candidate
Ye Su, Graduate Student
Brenda Tse, Research Coordinator
Moones Yadegari, Masters Student
Transforming childhood cancer care across the country
BC Children's Hospital clinicians and researchers are part of a new Canadian Pediatric Cancer Consortium that aims to transform pediatric cancer care and research by ensuring every child diagnosed with cancer in Canada will have increased and equitable access to therapies and care.
