- Overview
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Timely and accurate diagnosis of infectious agents is of the utmost importance in treating pediatric patients. I am interested in exploring how new technologies can be applied to improve the diagnosis of infectious disease and enable the best possible care of children.
- Publications
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Empiric antibiotics for peripartum bacteremia: A chart review from a quaternary Canadian centre
International Journal of Gynecology and Obstetrics
Mohn, S.F. and Reimer, R. and Mar, N. and Katelieva, A. and Paquette, V. and Albert, A.Y.K. and Roberts, A. and Tilley, P. and McClymont, E. and Ting, J. and Boucoiran, I. and Elwood, C.
DOI: 10.1002/ijgo.15048
2024Extraction-free clinical detection of SARS-CoV-2 virus from saline gargle samples using Hamilton STARlet liquid handler
Scientific Reports
Gadkar, V.J. and Goldfarb, D.M. and Al-Rawahi, G.N. and Srigley, J.A. and Smailus, D.E. and Coope, R.J.N. and Pleasance, S. and Watson, N. and Chen, T. and Lam, S. and Hoang, L. and Tilley, P.A.G.
DOI: 10.1038/s41598-023-30993-2
2023Comprehensive human amniotic fluid metagenomics supports the sterile womb hypothesis
Scientific Reports
Wang, H.C. and Yang, G.X. and Hu, Y. and Lam, P. and Sangha, K. and Siciliano, D. and Swenerton, A. and Miller, R. and Tilley, P. and Von Dadelszen, P. and Kalyan, S. and Tang, P. and Patel, M.S.
DOI: 10.1038/s41598-022-10869-7
2022Limitation of ctrA as a Target for Neisseria meningitidis Identification and Potential Alternative Targets
Journal of Clinical Microbiology
Sirluck-Schroeder, I. and Al-Rawahi, G.N. and Gadkar, V. and Hoang, L. and Tsang, R. and Tilley, P.
DOI: 10.1128/jcm.00152-22
2022Evaluation of observed and unobserved self-collection of saline gargle samples for the detection of SARS-CoV-2 in outpatients
Diagnostic Microbiology and Infectious Disease
Kinshella, M.-L.W. and Tilley, P. and Al-Rawahi, G.N. and Srigley, J.A. and Kayda, I. and Canes, M. and McLennan, M. and Bone, J.N. and Dittrick, M. and Gadkar, V.J. and Hoang, L.M.N. and Goldfarb, D.M.
DOI: 10.1016/j.diagmicrobio.2021.115566
2022Development and validation of a new triplex real-time quantitative reverse Transcriptase-PCR assay for the clinical detection of SARS-CoV-2
Molecular and Cellular Probes
Gadkar, V.J. and Goldfarb, D.M. and Young, V. and Watson, N. and Al-Rawahi, G.N. and Srigley, J. and Tilley, P.
DOI: 10.1016/j.mcp.2021.101744
2021Self-collected saline gargle samples as an alternative to health care worker-collected nasopharyngeal swabs for COVID-19 diagnosis in outpatients
Journal of Clinical Microbiology
Goldfarb, D.M. and Tilley, P. and Al-Rawahi, G.N. and Srigley, J.A. and Ford, G. and Pedersen, H. and Pabbi, A. and Hannam-Clark, S. and Charles, M. and Dittrick, M. and Gadkar, V.J. and Pernica, J.M. and Hoanga, L.M.N.
DOI: 10.1128/JCM.02427-20
2021Self-collected saline gargle samples as an alternative to healthcare worker collected nasopharyngeal swabs for COVID-19 diagnosis in outpatients
medRxiv
Goldfarb, D.M. and Tilley, P. and Al-Rawahi, G.N. and Srigley, J.A. and Ford, G. and Pedersen, H. and Pabbi, A. and Hannam-Clark, S. and Charles, M.K. and Dittrick, M. and Gadkar, V.J. and Pernica, J.M. and Hoang, L.M.N.
DOI: 10.1101/2020.09.13.20188334
2020Development of a national neonatal intensive care unit-specific antimicrobial stewardship programme in Canada: Protocol for a cohort study
BMJ Open
Ting, J.Y. and Roberts, A. and Tilley, P. and Robinson, J.L. and Dunn, M.S. and Paquette, V. and Lee, K.-S. and Shah, V. and Yoon, E. and Richter, L.L. and Lodha, A. and Shivananda, S. and Thampi, N. and Autmizguine, J. and Shah, P.S.
DOI: 10.1136/bmjopen-2020-043403
2020Matrix-assisted laser desorption/ionization time-of-flight MS for the accurate identification of Burkholderia cepacia complex and Burkholderia gladioli in the clinical microbiology laboratory
Journal of Medical Microbiology
Wong, K.S.K. and Dhaliwal, S. and Bilawka, J. and Srigley, J.A. and Champagne, S. and Romney, M.G. and Tilley, P. and Sadarangani, M. and Zlosnik, J.E.A. and Chilvers, M.A.
DOI: 10.1099/jmm.0.001223
2020Gargle-Direct: Extraction-free detection of SARS-CoV-2 using real-time PCR (RT-qPCR) of saline gargle rinse samples
medRxiv
Gadkar, V.J. and Goldfarb, D.M. and Young, V. and Watson, N. and Hoang, L. and Lee, T. and Prystajecky, N. and Al-Rawahi, G.N. and Srigley, J.A. and Tilley, P.
DOI: 10.1101/2020.10.09.20203430
2020Performance of the BD phoenix automated microbiology system for trimethoprim-sulfamethoxazole susceptibility testing of Staphylococcus aureus
Journal of Clinical Microbiology
Al-Rawahi, G.N. and Chorlton, S. and Dhaliwal, S. and Golding, G.R. and Tilley, P.
DOI: 10.1128/JCM.00994-19
2020A metagenomics-based diagnostic approach for central nervous system infections in hospital acute care setting
Scientific Reports
Hasan, M.R. and Sundararaju, S. and Tang, P. and Tsui, K.-M. and Lopez, A.P. and Janahi, M. and Tan, R. and Tilley, P.
DOI: 10.1038/s41598-020-68159-z
2020Third-generation cephalosporin-resistant urinary tract infections in children presenting to the paediatric emergency department
Paediatrics and Child Health (Canada)
Kang, K.T. and Ng, K. and Kendrick, J. and Tilley, P. and Ting, J. and Rassekh, S. and Murthy, S. and Roberts, A.
DOI: 10.1093/pch/pxy175
2020A Metagenomics – Based Diagnostic Approach for Central Nervous System Infections in Hospital Acute Care Setting
bioRxiv
Hasan, M.R. and Sundararaju, S. and Tang, P. and Tsui, K.-M. and Lopez, A.P. and Janahi, M. and Tan, R. and Tilley, P.
DOI: 10.1101/752618
2019Pediatric oncology and stem cell transplant patients with healthcare-associated Clostridium difficile infection were already colonized on admission
Pediatric Blood and Cancer
Al-Rawahi, G.N. and Al-Najjar, A. and McDonald, R. and Deyell, R.J. and Golding, G.R. and Brant, R. and Tilley, P. and Thomas, E. and Rassekh, S.R. and O'Gorman, A. and Wong, P. and Turnham, L. and Dobson, S.
DOI: 10.1002/pbc.27604
2019Running out of options: Extensively drug-resistant urinary tract infection in an infant
Paediatrics and Child Health (Canada)
Mijovic, H. and Wakeham, S. and Ng, K. and Al-Rawahi, G.N. and Tilley, P. and Ho, L. and Roberts, A.
DOI: 10.1093/pch/pxz048
2019Asymptomatic Pharyngeal Carriage of Kingella kingae among Young Children in Vancouver, British Columbia, Canada
Pediatric Infectious Disease Journal
Masud, S. and Greenman, J. and Mulpuri, K. and Hasan, M.R. and Goldfarb, D.M. and Tilley, P. and Gadkar, V.J. and Al-Rawahi, G.N.
DOI: 10.1097/INF.0000000000002403
2019Reduction of Inappropriate Antimicrobial Prescriptions in a Tertiary Neonatal Intensive Care Unit After Antimicrobial Stewardship Care Bundle Implementation
Pediatric Infectious Disease Journal
Ting, J.Y. and Paquette, V. and Ng, K. and Lisonkova, S. and Hait, V. and Shivanada, S. and Tilley, P. and Osiovich, H. and Roberts, A.
DOI: 10.1097/INF.0000000000002039
2019A novel real-time PCR assay panel for detection of common respiratory pathogens in a convenient, strip-tube array format
bioRxiv
Hasan, M.R. and Mana, H.A. and Young, V. and Tang, P. and Thomas, E. and Tan, R. and Tilley, P.
DOI: 10.1101/455568
2018Recovery of cytomegalovirus DNA from newborn saliva samples by different methods
Journal of Clinical Virology
Goshen, O. and Goldfarb, D.M. and Book, L. and Tilley, P. and Gantt, S.
DOI: 10.1016/j.jcv.2018.05.001
2018Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes
Scientific Reports
Gadkar, V.J. and Goldfarb, D.M. and Gantt, S. and Tilley, P.A.G.
DOI: 10.1038/s41598-018-23930-1
2018Is it safe to re-access sodium bicarbonate bottles for use in minor surgery?
Journal of Pediatric Surgery
Bjornson, L. and Bucevska, M. and Tilley, P. and Verchere, C.
DOI: 10.1016/j.jpedsurg.2018.04.005
2018Cellular metabolism constrains innate immune responses in early human ontogeny
Nature Communications
Kan, B. and Michalski, C. and Fu, H. and Au, H.H.T. and Lee, K. and Marchant, E.A. and Cheng, M.F. and Anderson-Baucum, E. and Aharoni-Simon, M. and Tilley, P. and Mirmira, R.G. and Ross, C.J. and Luciani, D.S. and Jan, E. and Lavoie, P.M.
DOI: 10.1038/s41467-018-07215-9
2018Impact of matrix-assisted laser desorption and ionization time-of-flight and antimicrobial stewardship intervention on treatment of bloodstream infections in hospitalized children
Journal of the Pediatric Infectious Diseases Society
Malcolmson, C. and Ng, K. and Hughes, S. and Kissoon, N. and Schina, J. and Tilley, P.A. and Roberts, A.
DOI: 10.1093/jpids/piw033
2017Symptomatic Congenital Cytomegalovirus Infection Is Underdiagnosed in British Columbia
The Journal of Pediatrics
Brendan Sorichetti and Oran Goshen and Julie Pauwels and Frederick K. Kozak and Peter Tilley and Mel Krajden and Soren Gantt
DOI: 10.1016/j.jpeds.2015.10.069
02/2016Bacillus pumilus Septic Arthritis in a Healthy Child
Canadian Journal of Infectious Diseases and Medical Microbiology
Shivamurthy, V.M. and Gantt, S. and Reilly, C. and Tilley, P. and Guzman, J. and Tucker, L.
DOI: 10.1155/2016/3265037
2016Respiratory Presentation of Pediatric Patients in the 2014 Enterovirus D68 Outbreak
Canadian Respiratory Journal
Martin, G. and Li, R. and Cook, V.E. and Carwana, M. and Tilley, P. and Sauve, L. and Tang, P. and Kapur, A. and Yang, C.L.
DOI: 10.1155/2016/8302179
2016Paediatric infectious keratitis at tertiary referral centres in Vancouver, Canada
British Journal of Ophthalmology
Noureddin, G.S. and Sasaki, S. and Butler, A.L. and Tilley, P. and Roscoe, D. and Lyons, C.J. and Holland, S.P. and Yeung, S.N.
DOI: 10.1136/bjophthalmol-2015-308034
2016Depletion of human DNA in spiked clinical specimens for improvement of sensitivity of pathogen detection by next-generation sequencing
Journal of Clinical Microbiology
Hasan, M.R. and Rawat, A. and Tang, P. and Jithesh, P.V. and Thomas, E. and Tan, R. and Tilley, P.
DOI: 10.1128/JCM.03050-15
2016Comparative evaluation of laboratory developed real-time PCR assays and RealStar® BKV PCR Kit for quantitative detection of BK polyomavirus
Journal of Virological Methods
Hasan, M.R. and Tan, R. and Al-Rawahi, G. and Thomas, E. and Tilley, P.
DOI: 10.1016/j.jviromet.2016.04.009
2016Surgical masks reduce airborne spread of Pseudomonas aeruginosa in colonized patients with cystic fibrosis
American Journal of Respiratory and Critical Care Medicine
Driessche, K.V. and Hens, N. and Tilley, P. and Quon, B.S. and Chilvers, M.A. and De Groot, R. and Cotton, M.F. and Marais, B.J. and Speert, D.P. and Zlosnik, J.E.A.
DOI: 10.1164/rccm.201503-0481LE
2015Evaluation of amplification targets for the specific detection of Bordetella pertussis using real-time polymerase chain reaction
Canadian Journal of Infectious Diseases and Medical Microbiology
Hasan, M.R. and Tan, R. and Al-Rawahi, G.N. and Thomas, E. and Tilley, P.
DOI: 10.1155/2014/763128
2014Optimal use of MRSASelect and PCR to maximize sensitivity and specificity of MRSA detection
Current Microbiology
Hasan, M.R. and Brunstein, J.D. and Al-Rawahi, G. and Tan, R. and Thomas, E. and Tilley, P.
DOI: 10.1007/s00284-012-0241-1
2013Short-term stability of pathogen-specific nucleic acid targets in clinical samples
Journal of Clinical Microbiology
Hasan, M.R. and Tan, R. and Al-Rawahi, G.N. and Thomas, E. and Tilley, P.
DOI: 10.1128/JCM.02659-12
2012Successful approach to treatment of helicobacter bilis infection in x-linked agammaglobulinemia
Journal of Clinical Immunology
Turvey, S.E. and Leo, S.H. and Boos, A. and Deans, G.D. and Prendiville, J. and Crawford, R.I. and Senger, C. and Conley, M.E. and Tilley, P. and Junker, A. and Janz, L. and Azana, R. and Hoang, L. and Morton, T.L.
DOI: 10.1007/s10875-012-9750-8
2012Antimicrobial resistance in gonorrhea: The influence of epidemiologic and laboratory surveillance data on treatment guidelines: Alberta, Canada 2001-2007
Sexually Transmitted Diseases
Singh, A.E. and Plitt, S. and Boyington, C. and Sutherland, K. and Lovgren, M. and Tilley, P. and Read, R.
DOI: 10.1097/OLQ.0b013e3181aad9df
2009Surveillance of mosquito-borne viruses in alberta using reverse transcription polymerase chain reaction with generic primers
Journal of Medical Entomology
Pabbaraju, K. and Ho, K.C.F. and Wong, S. and Fox, J.D. and Kaplen, B. and Tyler, S. and Drebot, M. and Tilley, P.A.G.
DOI: 10.1603/033.046.0332
2009Molecular characterization of syphilis in patients in Canada: Azithromycin resistance and detection of Treponema pallidum DNA in whole-blood samples versus ulcerative swabs
Journal of Clinical Microbiology
Martin, I.E. and Tsang, R.S.W. and Sutherland, K. and Tilley, P. and Read, R. and Anderson, B. and Roy, C. and Singh, A.E.
DOI: 10.1128/JCM.02392-08
2009Microfluidic device for dielectrophoresis manipulation and electrodisruption of respiratory pathogen Bordetella pertussis
IEEE Transactions on Biomedical Engineering
De La Rosa, C. and Tilley, P.A. and Fox, J.D. and Kaler, K.V.I.S.
DOI: 10.1109/TBME.2008.923148
2008Cost effectiveness of enzyme immunoassay and immunoblot testing for the diagnosis of syphilis
International Journal of STD and AIDS
Chuck, A. and Ohinmaa, A. and Tilley, P. and Singh, A. and Jacobs, P.
DOI: 10.1258/ijsa.2007.007272
2008Comparison between the clinical and laboratory features of enterovirus and West Nile virus infections
Journal of Medical Virology
Middleton, J. and Lee, B.E. and Fox, J.D. and Tilley, P.A.G. and Robinson, J.L.
DOI: 10.1002/jmv.21184
2008Screening of organ and tissue donors for West Nile virus by nucleic acid amplification - A three year experience in Alberta
American Journal of Transplantation
Tilley, P.A.G. and Fox, J.D. and Lee, B. and Chui, L. and Preiksaitis, J.
DOI: 10.1111/j.1600-6143.2008.02365.x
2008Respiratory pathogens
Molecular Pathology in Clinical Practice
Fox, J.D. and Tilley, P.A.
DOI: 10.1007/978-0-387-33227-7_41
2007Integrated microfluidic systems for sample preparation and detection of respiratory pathogen Bordetella pertussis
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
De La Rosa, C. and Prakash, R. and Tilley, P.A. and Fox, J.D. and Kaler, K.V.I.S.
DOI: 10.1109/IEMBS.2007.4353796
2007External quality assessment of a DNA sequence-based scheme for epidemiological typing of Legionella pneumophila by an international network of laboratories
Journal of Clinical Microbiology
Afshar, B. and Fry, N.K. and Bellamy, W. and Underwood, A.P. and Harrison, T.G. and Indra, A. and Deplano, A. and Soetens, O. and Tilley, P. and Bernard, K. and Bangsborg, J. and Nielsen, S. and Etienne, J. and Mentula, S. and Lück, P.C. and Franzin, L. and Scaturro, M. and Visca, P. and Amemura-Maekawa, J. and Mossong, J. and Bruin, J.P. and Van Der Zwaluw, K. and Caugant, D.A. and Marques, T. and Lindsay, D.S. and Blanco, S. and Pelaz, C. and Fendukly, F. and Herrmann, B. and Gaia, V. and Lai, S. and Benson, R.F.
DOI: 10.1128/JCM.00898-07
2007Integrated microfluidic systems for sample preparation and detection of respiratory pathogen Bordetella pertussis.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
de la Rosa, C. and Prakash, R. and Tilley, P.A. and Fox, J.D. and Kaler, K.V.
2007Maculopapular rash and tremor are associated with West Nile fever and neurological syndromes
Journal of Neurology, Neurosurgery and Psychiatry
Tilley, P.A.G. and Fox, J.D. and Jayaraman, G.C. and Preiksaitis, J.K.
DOI: 10.1136/jnnp.2006.107862
2007Evaluation of real-time PCR for diagnosis of Bordetella pertussis infection
BMC Infectious Diseases
Knorr, L. and Fox, J.D. and Tilley, P.A.G. and Ahmed-Bentley, J.
DOI: 10.1186/1471-2334-6-62
2006Use of sequence-based typing for investigation of a case of nosocomial legionellosis
Journal of Medical Microbiology
Wong, S. and Pabbaraju, K. and Burk, V.F. and Broukhanski, G.C. and Fox, J. and Louie, T. and Mah, M.W. and Bernard, K. and Tilley, P.A.G.
DOI: 10.1099/jmm.0.46754-0
2006Nucleic acid testing for West Nile virus RNA in plasma enhances rapid diagnosis of acute infection in symptomatic patients
Journal of Infectious Diseases
Tilley, P.A.G. and Fox, J.D. and Jayaraman, G.C. and Preiksaitis, J.K.
DOI: 10.1086/503577
2006West Nile virus infection and conjunctival exposure [8]
Emerging Infectious Diseases
Fonseca, K. and Prince, G.D. and Bratvold, J. and Fox, J.D. and Pybus, M. and Preksaitis, J.K. and Tilley, P.
DOI: 10.3201/eid1110.040212
2005The rash of West Nile virus infection
CMAJ. Canadian Medical Association Journal
Gorsche, R. and Tilley, P.
DOI: 10.1503/cmaj.1041013
2005Clinical utility of commercial enzyme immunoassays during the inaugural season of West Nile virus activity, Alberta, Canada
Journal of Clinical Microbiology
Tilley, P.A.G. and Walle, R. and Chow, A. and Jayaraman, G.C. and Fonseca, K. and Drebot, M.A. and Preiksaitis, J. and Fox, J.
DOI: 10.1128/JCM.43.9.4691-4695.2005
2005West Nile virus detection and commercial assays [7]
Emerging Infectious Diseases
Tilley, P.A.G. and Zachary, G.A. and Walle, R. and Schnee, P.F.
DOI: 10.3201/eid1107.041149
2005West Nile virus in Canadian blood donors
Transfusion
Cameron, C. and Reeves, J. and Antonishyn, N. and Tilley, P. and Alport, T. and Enrich, B. and Towns, D. and Lane, D. and Saldanha, J.
DOI: 10.1111/j.0041-1132.2005.04384.x
2005A novel Shigella dysenteriae serovar isolated in Canada
Journal of Clinical Microbiology
Melito, P.L. and Woodward, D.L. and Munro, J. and Walsh, J. and Foster, R. and Tilley, P. and Paccagnella, A. and Isaac-Renton, J. and Ismail, J. and Ng, L.K.
DOI: 10.1128/JCM.43.2.740-744.2005
2005Calgary experience with West Nile virus neurological syndrome during the late summer of 2003
Canadian Journal of Neurological Sciences
Sayao, A.-L. and Suchowersky, O. and Al-Khathaami, A. and Klassen, B. and Katz, N.R. and Sevick, R. and Tilley, P. and Fox, J. and Patry, D.
DOI: 10.1017/S031716710005383X
2004Relative Versus Absolute Noncontagiousness of Respiratory Tuberculosis on Treatment
Infection Control and Hospital Epidemiology
Long, R. and Bochar, K. and Chomyc, S. and Talbot, J. and Barrie, J. and Kunimoto, D. and Tilley, P.
DOI: 10.1086/502145
2003Association between handling of pet treats and infection with Salmonella enterica serotype Newport expressing the AmpC ß-lactamase, CMY-2
Journal of Clinical Microbiology
Pitout, J.D.D. and Reisbig, M.D. and Mulvey, M. and Chui, L. and Louie, M. and Crowe, L. and Church, D.L. and Elsayed, S. and Gregson, D. and Ahmed, R. and Tilley, P. and Hanson, N.D.
DOI: 10.1128/JCM.41.10.4578-4582.2003
2003Detection of Bordetella pertussis in a clinical laboratory by culture, polymerase chain reaction, and direct fluorescent antibody staining; accuracy, and cost
Diagnostic Microbiology and Infectious Disease
Tilley, P.A.G. and Kanchana, M.V. and Knight, I. and Blondeau, J. and Antonishyn, N. and Deneer, H.
DOI: 10.1016/S0732-8893(00)00117-6
2000Detection of Mycobacterium-specific interferon-gamma-producing human T lymphocytes by flow cytometry
APMIS
Tilley, P.A.G. and Menon, J.N.
DOI: 10.1034/j.1600-0463.2000.d01-6.x
2000Bacteremia with acinetobacter species: Risk factors and prognosis in different clinical settings
Clinical Infectious Diseases
Tilley, P.A.G. and Roberts, F.J.
DOI: 10.1093/clinids/18.6.896
1994Varicella-zoster virus antibody avidity and IgG-subclass patterns in children with recurrent chickenpox
Journal of Medical Virology
Junker, A.K. and Tilley, P.
DOI: 10.1002/jmv.1890430204
1994Three cases of relapsing fever associated with lakeside cabins in Idaho.
Canada communicable disease report = Relevé des maladies transmissibles au Canada
Tilley, P.A. and Azar, R. and Banerjee, S. and Bell, A.
1994Interleukin-2 (r-IL-2)-induced lymphokine-activated killer (LAK) cells and their precursors express the VGO1 antigen
Journal of Clinical Immunology
Denegri, J.F. and Peterson, J. and Tilley, P.
DOI: 10.1007/BF00918668
1989 - Research
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Current Projects
Early and accurate diagnosis of respiratory and central nervous system infections can lead to improved outcomes and earlier discharges, yet identification methods for such diseases are commonly available for only a limited number of infectious agents. Next generation sequencing (NGS) technology has shown promise for detection of a huge range of human pathogens from biological samples. I have been involved as a co-investigator in a pilot study examining a new lab process for NGS for the comprehensive detection of bacterial and viral pathogens in pediatric respiratory and cerebrospinal fluid samples.GrantsPrincipal Investigator R Hasan. Application of Next Generation Sequencing to the diagnosis of respiratory illness in critically ill children.
B.C. Children’s Hospital Telethon Grant Competition. $29,869 Sept 2012.
Research Roundup: January 2022
This month’s Research Roundup covers how increasing parent-child proximity in the first days of life can improve toddler behaviour later on, what lean body mass can tell us about the health of pediatric kidney transplant recipients, insights into the barriers to knowledge translation in Canadian pediatric health-care centres, and an evaluation of the effectiveness of saline gargle COVID-19 tests.
