- Overview
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Timely and accurate diagnosis of infectious agents is of the utmost importance in treating pediatric patients. I am interested in exploring how new technologies can be applied to improve the diagnosis of infectious disease and enable the best possible care of children.
- Publications
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Empiric antibiotics for peripartum bacteremia: A chart review from a quaternary Canadian centre
International Journal of Gynecology and Obstetrics
DOI: 10.1002/ijgo.15048
2024Extraction-free clinical detection of SARS-CoV-2 virus from saline gargle samples using Hamilton STARlet liquid handler
Scientific Reports
DOI: 10.1038/s41598-023-30993-2
2023Comprehensive human amniotic fluid metagenomics supports the sterile womb hypothesis
Scientific Reports
DOI: 10.1038/s41598-022-10869-7
2022Limitation of ctrA as a Target for Neisseria meningitidis Identification and Potential Alternative Targets
Journal of Clinical Microbiology
DOI: 10.1128/jcm.00152-22
2022Evaluation of observed and unobserved self-collection of saline gargle samples for the detection of SARS-CoV-2 in outpatients
Diagnostic Microbiology and Infectious Disease
DOI: 10.1016/j.diagmicrobio.2021.115566
2022Development and validation of a new triplex real-time quantitative reverse Transcriptase-PCR assay for the clinical detection of SARS-CoV-2
Molecular and Cellular Probes
DOI: 10.1016/j.mcp.2021.101744
2021Self-collected saline gargle samples as an alternative to health care worker-collected nasopharyngeal swabs for COVID-19 diagnosis in outpatients
Journal of Clinical Microbiology
DOI: 10.1128/JCM.02427-20
2021Self-collected saline gargle samples as an alternative to healthcare worker collected nasopharyngeal swabs for COVID-19 diagnosis in outpatients
medRxiv
DOI: 10.1101/2020.09.13.20188334
2020Development of a national neonatal intensive care unit-specific antimicrobial stewardship programme in Canada: Protocol for a cohort study
BMJ Open
DOI: 10.1136/bmjopen-2020-043403
2020Matrix-assisted laser desorption/ionization time-of-flight MS for the accurate identification of Burkholderia cepacia complex and Burkholderia gladioli in the clinical microbiology laboratory
Journal of Medical Microbiology
DOI: 10.1099/jmm.0.001223
2020Gargle-Direct: Extraction-free detection of SARS-CoV-2 using real-time PCR (RT-qPCR) of saline gargle rinse samples
medRxiv
DOI: 10.1101/2020.10.09.20203430
2020Performance of the BD phoenix automated microbiology system for trimethoprim-sulfamethoxazole susceptibility testing of Staphylococcus aureus
Journal of Clinical Microbiology
DOI: 10.1128/JCM.00994-19
2020A metagenomics-based diagnostic approach for central nervous system infections in hospital acute care setting
Scientific Reports
DOI: 10.1038/s41598-020-68159-z
2020Third-generation cephalosporin-resistant urinary tract infections in children presenting to the paediatric emergency department
Paediatrics and Child Health (Canada)
DOI: 10.1093/pch/pxy175
2020A Metagenomics – Based Diagnostic Approach for Central Nervous System Infections in Hospital Acute Care Setting
bioRxiv
DOI: 10.1101/752618
2019Pediatric oncology and stem cell transplant patients with healthcare-associated Clostridium difficile infection were already colonized on admission
Pediatric Blood and Cancer
DOI: 10.1002/pbc.27604
2019Running out of options: Extensively drug-resistant urinary tract infection in an infant
Paediatrics and Child Health (Canada)
DOI: 10.1093/pch/pxz048
2019Asymptomatic Pharyngeal Carriage of Kingella kingae among Young Children in Vancouver, British Columbia, Canada
Pediatric Infectious Disease Journal
DOI: 10.1097/INF.0000000000002403
2019Reduction of Inappropriate Antimicrobial Prescriptions in a Tertiary Neonatal Intensive Care Unit After Antimicrobial Stewardship Care Bundle Implementation
Pediatric Infectious Disease Journal
DOI: 10.1097/INF.0000000000002039
2019A novel real-time PCR assay panel for detection of common respiratory pathogens in a convenient, strip-tube array format
bioRxiv
DOI: 10.1101/455568
2018Recovery of cytomegalovirus DNA from newborn saliva samples by different methods
Journal of Clinical Virology
DOI: 10.1016/j.jcv.2018.05.001
2018Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes
Scientific Reports
DOI: 10.1038/s41598-018-23930-1
2018Is it safe to re-access sodium bicarbonate bottles for use in minor surgery?
Journal of Pediatric Surgery
DOI: 10.1016/j.jpedsurg.2018.04.005
2018Cellular metabolism constrains innate immune responses in early human ontogeny
Nature Communications
DOI: 10.1038/s41467-018-07215-9
2018Impact of matrix-assisted laser desorption and ionization time-of-flight and antimicrobial stewardship intervention on treatment of bloodstream infections in hospitalized children
Journal of the Pediatric Infectious Diseases Society
DOI: 10.1093/jpids/piw033
2017Symptomatic Congenital Cytomegalovirus Infection Is Underdiagnosed in British Columbia
The Journal of Pediatrics
Brendan Sorichetti and Oran Goshen and Julie Pauwels and Frederick K. Kozak and Peter Tilley and Mel Krajden and Soren Gantt
DOI: 10.1016/j.jpeds.2015.10.069
02/2016Bacillus pumilus Septic Arthritis in a Healthy Child
Canadian Journal of Infectious Diseases and Medical Microbiology
DOI: 10.1155/2016/3265037
2016Respiratory Presentation of Pediatric Patients in the 2014 Enterovirus D68 Outbreak
Canadian Respiratory Journal
DOI: 10.1155/2016/8302179
2016Paediatric infectious keratitis at tertiary referral centres in Vancouver, Canada
British Journal of Ophthalmology
DOI: 10.1136/bjophthalmol-2015-308034
2016Depletion of human DNA in spiked clinical specimens for improvement of sensitivity of pathogen detection by next-generation sequencing
Journal of Clinical Microbiology
DOI: 10.1128/JCM.03050-15
2016Comparative evaluation of laboratory developed real-time PCR assays and RealStar® BKV PCR Kit for quantitative detection of BK polyomavirus
Journal of Virological Methods
DOI: 10.1016/j.jviromet.2016.04.009
2016Surgical masks reduce airborne spread of Pseudomonas aeruginosa in colonized patients with cystic fibrosis
American Journal of Respiratory and Critical Care Medicine
DOI: 10.1164/rccm.201503-0481LE
2015Evaluation of amplification targets for the specific detection of Bordetella pertussis using real-time polymerase chain reaction
Canadian Journal of Infectious Diseases and Medical Microbiology
DOI: 10.1155/2014/763128
2014Optimal use of MRSASelect and PCR to maximize sensitivity and specificity of MRSA detection
Current Microbiology
DOI: 10.1007/s00284-012-0241-1
2013Short-term stability of pathogen-specific nucleic acid targets in clinical samples
Journal of Clinical Microbiology
DOI: 10.1128/JCM.02659-12
2012Successful approach to treatment of helicobacter bilis infection in x-linked agammaglobulinemia
Journal of Clinical Immunology
DOI: 10.1007/s10875-012-9750-8
2012Antimicrobial resistance in gonorrhea: The influence of epidemiologic and laboratory surveillance data on treatment guidelines: Alberta, Canada 2001-2007
Sexually Transmitted Diseases
DOI: 10.1097/OLQ.0b013e3181aad9df
2009Surveillance of mosquito-borne viruses in alberta using reverse transcription polymerase chain reaction with generic primers
Journal of Medical Entomology
DOI: 10.1603/033.046.0332
2009Molecular characterization of syphilis in patients in Canada: Azithromycin resistance and detection of Treponema pallidum DNA in whole-blood samples versus ulcerative swabs
Journal of Clinical Microbiology
DOI: 10.1128/JCM.02392-08
2009Microfluidic device for dielectrophoresis manipulation and electrodisruption of respiratory pathogen Bordetella pertussis
IEEE Transactions on Biomedical Engineering
DOI: 10.1109/TBME.2008.923148
2008Cost effectiveness of enzyme immunoassay and immunoblot testing for the diagnosis of syphilis
International Journal of STD and AIDS
DOI: 10.1258/ijsa.2007.007272
2008Comparison between the clinical and laboratory features of enterovirus and West Nile virus infections
Journal of Medical Virology
DOI: 10.1002/jmv.21184
2008Screening of organ and tissue donors for West Nile virus by nucleic acid amplification - A three year experience in Alberta
American Journal of Transplantation
DOI: 10.1111/j.1600-6143.2008.02365.x
2008Respiratory pathogens
Molecular Pathology in Clinical Practice
DOI: 10.1007/978-0-387-33227-7_41
2007Integrated microfluidic systems for sample preparation and detection of respiratory pathogen Bordetella pertussis
Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
DOI: 10.1109/IEMBS.2007.4353796
2007External quality assessment of a DNA sequence-based scheme for epidemiological typing of Legionella pneumophila by an international network of laboratories
Journal of Clinical Microbiology
DOI: 10.1128/JCM.00898-07
2007Integrated microfluidic systems for sample preparation and detection of respiratory pathogen Bordetella pertussis.
Conference proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference
2007Maculopapular rash and tremor are associated with West Nile fever and neurological syndromes
Journal of Neurology, Neurosurgery and Psychiatry
DOI: 10.1136/jnnp.2006.107862
2007Evaluation of real-time PCR for diagnosis of Bordetella pertussis infection
BMC Infectious Diseases
DOI: 10.1186/1471-2334-6-62
2006Use of sequence-based typing for investigation of a case of nosocomial legionellosis
Journal of Medical Microbiology
DOI: 10.1099/jmm.0.46754-0
2006Nucleic acid testing for West Nile virus RNA in plasma enhances rapid diagnosis of acute infection in symptomatic patients
Journal of Infectious Diseases
DOI: 10.1086/503577
2006West Nile virus infection and conjunctival exposure [8]
Emerging Infectious Diseases
DOI: 10.3201/eid1110.040212
2005The rash of West Nile virus infection
CMAJ. Canadian Medical Association Journal
DOI: 10.1503/cmaj.1041013
2005Clinical utility of commercial enzyme immunoassays during the inaugural season of West Nile virus activity, Alberta, Canada
Journal of Clinical Microbiology
DOI: 10.1128/JCM.43.9.4691-4695.2005
2005West Nile virus detection and commercial assays [7]
Emerging Infectious Diseases
DOI: 10.3201/eid1107.041149
2005West Nile virus in Canadian blood donors
Transfusion
DOI: 10.1111/j.0041-1132.2005.04384.x
2005A novel Shigella dysenteriae serovar isolated in Canada
Journal of Clinical Microbiology
DOI: 10.1128/JCM.43.2.740-744.2005
2005Calgary experience with West Nile virus neurological syndrome during the late summer of 2003
Canadian Journal of Neurological Sciences
DOI: 10.1017/S031716710005383X
2004Relative Versus Absolute Noncontagiousness of Respiratory Tuberculosis on Treatment
Infection Control and Hospital Epidemiology
DOI: 10.1086/502145
2003Association between handling of pet treats and infection with Salmonella enterica serotype Newport expressing the AmpC ß-lactamase, CMY-2
Journal of Clinical Microbiology
DOI: 10.1128/JCM.41.10.4578-4582.2003
2003Detection of Bordetella pertussis in a clinical laboratory by culture, polymerase chain reaction, and direct fluorescent antibody staining; accuracy, and cost
Diagnostic Microbiology and Infectious Disease
DOI: 10.1016/S0732-8893(00)00117-6
2000Detection of Mycobacterium-specific interferon-gamma-producing human T lymphocytes by flow cytometry
APMIS
DOI: 10.1034/j.1600-0463.2000.d01-6.x
2000Bacteremia with acinetobacter species: Risk factors and prognosis in different clinical settings
Clinical Infectious Diseases
DOI: 10.1093/clinids/18.6.896
1994Varicella-zoster virus antibody avidity and IgG-subclass patterns in children with recurrent chickenpox
Journal of Medical Virology
DOI: 10.1002/jmv.1890430204
1994Three cases of relapsing fever associated with lakeside cabins in Idaho.
Canada communicable disease report = Relevé des maladies transmissibles au Canada
1994Interleukin-2 (r-IL-2)-induced lymphokine-activated killer (LAK) cells and their precursors express the VGO1 antigen
Journal of Clinical Immunology
DOI: 10.1007/BF00918668
1989 - Research
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Current Projects
Early and accurate diagnosis of respiratory and central nervous system infections can lead to improved outcomes and earlier discharges, yet identification methods for such diseases are commonly available for only a limited number of infectious agents. Next generation sequencing (NGS) technology has shown promise for detection of a huge range of human pathogens from biological samples. I have been involved as a co-investigator in a pilot study examining a new lab process for NGS for the comprehensive detection of bacterial and viral pathogens in pediatric respiratory and cerebrospinal fluid samples.GrantsPrincipal Investigator R Hasan. Application of Next Generation Sequencing to the diagnosis of respiratory illness in critically ill children.
B.C. Children’s Hospital Telethon Grant Competition. $29,869 Sept 2012.
Research Roundup: January 2022
This month’s Research Roundup covers how increasing parent-child proximity in the first days of life can improve toddler behaviour later on, what lean body mass can tell us about the health of pediatric kidney transplant recipients, insights into the barriers to knowledge translation in Canadian pediatric health-care centres, and an evaluation of the effectiveness of saline gargle COVID-19 tests.
