My research focuses on development and improvement of biochemical laboratory measures for improved diagnoses of both maternal and neonatal health issues.
Comparative evaluation of neonatal bilirubin using Radiometer whole blood co-oximetry and plasma bilirubin methods from Roche Diagnostics and Ortho Clinical Diagnostics
Ian Marie Lano and Andrew W. Lyon and Li Wang and Rob Ruskin and Martha E. Lyon
Limitations and opportunities of whole blood bilirubin measurements by GEM premier 4000®
Li Wang and Arianne Y. K. Albert and Benjamin Jung and Keyvan Hadad and Martha E. Lyon and Melanie Basso
Visual or automated dipstick testing for proteinuria in pregnancy?
Pregnancy Hypertension: An International Journal of Women's Cardiovascular Health
Mauricio E. Correa and Anne-Marie Côté and Dane A. De Silva and Li Wang and Prianthiny Packianathan and Peter von Dadelszen and Laura A. Magee
Roche acetaminophen assay negative bias at low concentrations is imprvoved using the TDM diluent as the zero calibrator
P. Kavsak and N. Wakefied and L. Clark and L. Lyko and R. Pickersgill and L. Wang and J. Zeidler
Rescue of Munc18-1 and -2 Double Knockdown Reveals the Essential Functions of Interaction between Munc18 and Closed Syntaxin in PC12 Cells
Molecular Biology of the Cell
L. Han and T. Jiang and G. A. Han and N. T. Malintan and L. Xie and L. Wang and F. W. Tse and H. Y. Gaisano and B. M. Collins and F. A. Meunier and S. Sugita
RalA and RalB Function as the Critical GTP Sensors for GTP-Dependent Exocytosis
Journal of Neuroscience
G. Li and L. Han and T.-C. Chou and Y. Fujita and L. Arunachalam and A. Xu and A. Wong and S.-K. Chiew and Q. Wan and L. Wang and S. Sugita
A Central Kinase Domain of Type I Phosphatidylinositol Phosphate Kinases Is Sufficient to Prime Exocytosis: ISOFORM SPECIFICITY AND ITS UNDERLYING MECHANISM
Journal of Biological Chemistry
L. Wang and G. Li and S. Sugita
Whole blood measurement of bilirubin: Bilirubin is routinely measured in infants before discharge to screen for hyperbilirubinemia (jaundice). Early detection prevents brain damage that may occur with high levels of bilirubin. I am currently investigating new methods to assess bilirubin with whole blood. This project compares whole blood bilirubin measurement to plasma bilirubin measurement to verify accuracy, efficiency and quality and assess factors which might interfere with the whole blood method. If successful, this will enable assessment using much smaller blood sample volumes and producing faster results at the point of care.
Biomarkers of preeclampsia: Early diagnosis of preeclampsia can potentially prevent some of the adverse outcomes associated with this disease. However, early diagnosis of this disease is currently limited by a lack of clinical tests in this area. There is increasing evidence that an enzyme that breaks down tryptophan, an amino acid essential in the diet, is involved in the development of preeclampsia. I am assessing the levels of tryptophan and its metabolites in maternal blood to see whether they can be used to identify patients with preeclampsia in the second trimester of pregnancy before preeclampsia would typically be diagnosed. The development of a new test to predict preeclampsia and its complications will help clinicians provide an accurate diagnosis early in pregnancy and modify patient care accordingly, thus allowing them to provide the best care for both mothers and babies.Grants
Ultra performance liquid chromatography-Tandem mass spectrometry (UPLC-MS/MS) determination of tryptophan and kynurenines in urine and plasma samples. Resident Research Grant, Department of Pathology & Molecular Medicine, McMaster University (2011).
Evaluation of the role of maternal tryptophan and kynurenine pathway metabolites in early diagnosis of preeclampsia. Clinical Translational Research Seed Grant, Child & Family Research Institute (2014-2015).