As a neonatologist, I am regularly involved in the medical care of infants needing intensive medical care at birth. Newborns are particularly vulnerable to infections. Infection and prematurity are leading causes of mortality and long-term health problems. My lab focuses on understanding the development of the immune system in humans before and at birth, and how to use this knowledge to prevent diseases in this age group.

We also work on understanding why some human individuals are more susceptible to infections or auto-immune/inflammatory conditions compared to others, in the general population and across development. To do so, we employ a combination of translational research approaches (population-based clinical studies, epidemiology, but lab-based human immunology and molecular biology, but also clinical trials/interventional studies. We also have ongoing collaboration nationally and internationally (US/Europe) to understand risk factors for neonatal infections, genetics of BPD, HIV, vaccine development, microbiome and human immunology traits.


Glucose control and autonomic response during acute stress in youth with type 1 diabetes: A pilot study
Pediatric Diabetes
Ling Guan and Daniel L Metzger and Pascal M Lavoie and Jean-Paul Collet
DOI: 10.1111/pedi.12680

A Time-Based Analysis of Inflammation in Infants at Risk of Bronchopulmonary Dysplasia
The Journal of Pediatrics
Sandrine Leroy and Elsa Caumette and Chandra Waddington and Audrey Hébert and Rollin Brant and Pascal M. Lavoie
DOI: 10.1016/j.jpeds.2017.09.011

Why haven't the documented benefits of probiotics on preterm babies led to their wider acceptance and use?
Acta Paediatrica
Constantin R. Popescu and Leah T. Stiemsma and Pascal M. Lavoie
DOI: 10.1111/apa.13893

Genome-wide association study of bronchopulmonary dysplasia: a potential role for variants near the CRP gene
Scientific Reports
Mari Mahlman and Minna K. Karjalainen and Johanna M. Huusko and Sture Andersson and M. Anneli Kari and Outi K. T. Tammela and Ulla Sankilampi and Liisa Lehtonen and Riitta H. Marttila and Dirk Bassler and Christian F. Poets and Thierry Lacaze-Masmonteil and Claude Danan and Christophe Delacourt and Aarno Palotie and Louis J. Muglia and Pascal M. Lavoie and Alice Hadchouel and Mika Rämet and Mikko Hallman
DOI: 10.1038/s41598-017-08977-w

The phenotype and function of preterm infant monocytes: implications for susceptibility to infection
Journal of Leukocyte Biology
Emma de Jong and Tobias Strunk and David Burgner and Pascal M. Lavoie and Andrew Currie
DOI: 10.1189/jlb.4ru0317-111r

Cord blood hematopoietic cells from preterm infants display altered DNA methylation patterns
Clinical Epigenetics
Olivia M. de Goede and Pascal M. Lavoie and Wendy P. Robinson
DOI: 10.1186/s13148-017-0339-1

Respiratory syncytial virus-neutralizing serum antibody titers in infants following palivizumab prophylaxis with an abbreviated dosing regimen
Jennifer Claydon and Amitava Sur and Allison Callejas and Mihoko Ladd and Eddie Kwan and Richard Taylor and Stuart E. Turvey and Alfonso Solimano and Pascal M. Lavoie and Nico Marr
DOI: 10.1371/journal.pone.0176152

Antifungal Immunological Defenses in Newborns
Frontiers in Immunology
Christina Michalski and Bernard Kan and Pascal M. Lavoie
DOI: 10.3389/fimmu.2017.00281

Mononuclear Phagocyte System
Fetal and Neonatal Physiology
Pascal M. Lavoie and Ofer Levy
DOI: 10.1016/b978-0-323-35214-7.00125-6

A Potential Role for the NOD1 Variant (rs6958571) in Gram-Positive Blood Stream Infection in ELBW Infants
Venkatesh Sampath and Neil Mulrooney and Aloka L. Patel and Jonathan Cohen and Liyun Zhang and Jeffery Garland and Pascal M. Lavoie and John Dagle and Pippa Simpson
DOI: 10.1159/000477433

Characterizing the hypomethylated DNA methylation profile of nucleated red blood cells from cord blood
Olivia M de Goede and Pascal M Lavoie and Wendy P Robinson
DOI: 10.2217/epi-2016-0069

A functional ATG16L1 (T300A) variant is associated with necrotizing enterocolitis in premature infants
Pediatric Research
Venkatesh Sampath and Vineet Bhandari and Jessica Berger and Daniel Merchant and Liyun Zhang and Mihoko Ladd and Heather Menden and Jeffery Garland and Namasivayam Ambalavanan and Neil Mulrooney and Michael Quasney and John Dagle and Pascal M. Lavoie and Pippa Simpson and Mary Dahmer
DOI: 10.1038/pr.2016.260

An Immunological Perspective on Neonatal Sepsis
Trends in Molecular Medicine
Bernard Kan and Hamid Reza Razzaghian and Pascal M. Lavoie
DOI: 10.1016/j.molmed.2016.02.001

Outcomes of Respiratory Syncytial Virus Immunoprophylaxis in Infants Using an Abbreviated Dosing Regimen of Palivizumab
JAMA Pediatrics
Pascal M. Lavoie and Alfonso Solimano and Richard Taylor and Eddie Kwan and Jennifer Claydon and Stuart E. Turvey and Nico Marr
DOI: 10.1001/jamapediatrics.2015.3235

Multiple Successful Angioembolizations for Refractory Cardiac Failure in a Preterm with Rapidly Involuting Congenital Hemangioma
American Journal of Perinatology Reports
Amitava Sur and Heran Manraj and Pascal Lavoie and Ken Lim and Douglas Courtemanche and Paul Brooks and Susan Albersheim
DOI: 10.1055/s-0035-1570342

Genetics of Bronchopulmonary Dysplasia
Bronchopulmonary Dysplasia
Pascal M. Lavoie
DOI: 10.1007/978-3-319-28486-6_6

Cardiac Autonomic Function at Baseline and under Stress and Its Relationship to Circulatory Markers of Inflammation in Obese Compared to Nonobese Children: A Pilot Study
Hormone Research in Paediatrics
Brenden E. Hursh and Mir Sohail Fazeli and Sarah Wang and Elizabeth A. Marchant and Paula Woo and Rajavel Elango and Pascal M. Lavoie and Jean-Pierre Chanoine and Jean-Paul Collet
DOI: 10.1159/000445685

Editor’s Focus
Pediatric Research
Elizabeth A. Marchant and Bernard Kan and Ashish A. Sharma and Alice van Zanten and Tobias R. Kollmann and Rollin Brant and Pascal M. Lavoie
DOI: 10.1038/pr.2015.171

A Randomized Controlled Trial of the Use of Oral Glucose with or without Gentle Facilitated Tucking of Infants during Neonatal Echocardiography
Pascal M. Lavoie and Amelie Stritzke and Joseph Ting and Mohammad Jabr and Amish Jain and Eddie Kwan and Ela Chakkarapani and Paul Brooks and Rollin Brant and Patrick J. McNamara and Liisa Holsti
DOI: 10.1371/journal.pone.0141015

Nucleated red blood cells impact DNA methylation and expression analyses of cord blood hematopoietic cells
Clinical Epigenetics
Olivia M. de Goede and Hamid R. Razzaghian and E. Magda Price and Meaghan J. Jones and Michael S. Kobor and Wendy P. Robinson and Pascal M. Lavoie
DOI: 10.1186/s13148-015-0129-6

Attenuated innate immune defenses in very premature neonates during the neonatal period
Pediatric Research
Elizabeth A. Marchant and Bernard Kan and Ashish A. Sharma and Alice van Zanten and Tobias R. Kollmann and Rollin Brant and Pascal M. Lavoie
DOI: 10.1038/pr.2015.132

The Crohn’s disease-associated polymorphism in ATG16L1 (rs2241880) reduces SHIP gene expression and activity in human subjects
Genes & Immunity
E N Ngoh and H K Brugger and M Monajemi and S C Menzies and A F Hirschfeld and K L Del Bel and K Jacobson and P M Lavoie and S E Turvey and L M Sly
DOI: 10.1038/gene.2015.30

Malaria in immigrant and traveler children: Clinical presentation and risk factors for complications
Journal of Pediatric Infectious Diseases
Pascal Lavoie and Monique Robert and Marc Lebel and Dorothy Moore and Selim Rashed and Ana Carceller
DOI: 10.1055/s-0035-1557058

Oral glucose during targeted neonatal echocardiography: is it useful?: Table 1
Archives of Disease in Childhood - Fetal and Neonatal Edition
Pascal M Lavoie and Amelie Stritzke and Joseph Ting and Mohammad Jabr and Amish Jain and Eddie Kwan and Ela Chakkarapani and Paul Brooks and Rollin Brant and Patrick J McNamara and Liisa Holsti
DOI: 10.1136/archdischild-2015-308191

Genes Encoding Vascular Endothelial Growth Factor A (VEGF-A) and VEGF Receptor 2 (VEGFR-2) and Risk for Bronchopulmonary Dysplasia
Mari Mahlman and Johanna M. Huusko and Minna K. Karjalainen and Tuula Kaukola and Riitta Marttila and Marja Ojaniemi and Ritva Haataja and Pascal M. Lavoie and Mika Rämet and Mikko Hallman and on behalf of the Gen-BPD Study Group
DOI: 10.1159/000381279

Cerebral Palsy and Polymorphism of the Chemokine CCL18 in Very Preterm Children
Hanna Kallankari and Johanna M. Huusko and Tuula Kaukola and Marja Ojaniemi and Mari Mahlman and Riitta Marttila and Stephen F. Kingsmore and Leena Haataja and Pascal M. Lavoie and Anne Synnes and Mikko Hallman
DOI: 10.1159/000430765

A study of genes encoding cytokines (IL6, IL10, TNF), cytokine receptors (IL6R, IL6ST), and glucocorticoid receptor (NR3C1) and susceptibility to bronchopulmonary dysplasia
BMC Medical Genetics
Johanna M Huusko and Minna K Karjalainen and Mari Mahlman and Ritva Haataja and M Anneli Kari and Sture Andersson and Gergely Toldi and Outi Tammela and Mika Rämet and Pascal M Lavoie and Mikko Hallman
DOI: 10.1186/s12881-014-0120-7

Impaired NLRP3 inflammasome activity during fetal development regulates IL-1ß production in human monocytes
European Journal of Immunology
Ashish A. Sharma and Roger Jen and Bernard Kan and Abhinav Sharma and Elizabeth Marchant and Anthony Tang and Izabelle Gadawski and Christof Senger and Amanda Skoll and Stuart E. Turvey and Laura M. Sly and Hélène C.F. Côté and Pascal M. Lavoie
DOI: 10.1002/eji.201444707

Catecholamine-resistant hypotension and myocardial performance following patent ductus arteriosus ligation
Journal of Perinatology
S Noori and PDA Ligation/Hypotension Trial Investigators and P McNamara and A Jain and P M Lavoie and A Wickremasinghe and T A Merritt and T Solomon and K Sekar and J T Attridge and J R Swanson and M Gillam-Krakauer and J Reese and B B Poindexter and M Brook and R J Auchus and R I Clyman
DOI: 10.1038/jp.2014.151

Assessment of Genetic Associations between Common Single Nucleotide Polymorphisms in RIG-I-Like Receptor and IL-4 Signaling Genes and Severe Respiratory Syncytial Virus Infection in Children: A Candidate Gene Case-Control Study
Nico Marr and Aaron F. Hirschfeld and Angie Lam and Shirley Wang and Pascal M. Lavoie and Stuart E. Turvey
DOI: 10.1371/journal.pone.0100269

Hypotension following Patent Ductus Arteriosus Ligation: The Role of Adrenal Hormones
The Journal of Pediatrics
Ronald I. Clyman and Andrea Wickremasinghe and T. Allen Merritt and Tabitha Solomon and Patrick McNamara and Amish Jain and Jaideep Singh and Alison Chu and Shahab Noori and Krishnamurthy Sekar and Pascal M. Lavoie and Joshua T. Attridge and Jonathan R. Swanson and Maria Gillam-Krakauer and Jeff Reese and Sara DeMauro and Brenda Poindexter and Sue Aucott and Monique Satpute and Erika Fernandez and Richard J. Auchus
DOI: 10.1016/j.jpeds.2014.01.058

Omega-3 Long-Chain Polyunsaturated Fatty Acids for Extremely Preterm Infants: A Systematic Review
P. Zhang and P. M. Lavoie and T. Lacaze-Masmonteil and M. Rhainds and I. Marc
DOI: 10.1542/peds.2014-0459

Preferential Cephalic Redistribution of Left Ventricular Cardiac Output during Therapeutic Hypothermia for Perinatal Hypoxic-Ischemic Encephalopathy
The Journal of Pediatrics
Ori Hochwald and Mohammad Jabr and Horacio Osiovich and Steven P. Miller and Patrick J. McNamara and Pascal M. Lavoie
DOI: 10.1016/j.jpeds.2014.01.028

Surgical site infections in infants admitted to the neonatal intensive care unit
Journal of Pediatric Surgery
Ilan Segal and Christine Kang and Susan G. Albersheim and Erik D. Skarsgard and Pascal M. Lavoie
DOI: 10.1016/j.jpedsurg.2013.08.001

Polymorphisms of the gene encoding Kit ligand are associated with bronchopulmonary dysplasia
Pediatric Pulmonology
Johanna M. Huusko and Mari Mahlman and Minna K. Karjalainen and Tuula Kaukola and Ritva Haataja and Riitta Marttila and Gergely Toldi and Miklós Szabó and Stephen F. Kingsmore and Mika Rämet and Pascal M. Lavoie and Mikko Hallman and on behalf of Gen-BPD Study Group
DOI: 10.1002/ppul.23018

Attenuation of Respiratory Syncytial Virus-Induced and RIG-I-Dependent Type I IFN Responses in Human Neonates and Very Young Children
The Journal of Immunology
N. Marr and T.-I. Wang and S. H. Y. Kam and Y. S. Hu and A. A. Sharma and A. Lam and J. Markowski and A. Solimano and P. M. Lavoie and S. E. Turvey
DOI: 10.4049/jimmunol.1302007

Hierarchical Maturation of Innate Immune Defences in Very Preterm Neonates
Ashish Arunkumar Sharma and Roger Jen and Rollin Brant and Mihoko Ladd and Qing Huang and Amanda Skoll and Christof Senger and Stuart E. Turvey and Nico Marr and Pascal M. Lavoie
DOI: 10.1159/000358550

Neonatal Pain-Related Stress and NFKBIA Genotype Are Associated with Altered Cortisol Levels in Preterm Boys at School Age
Ruth E. Grunau and Ivan L. Cepeda and Cecil M. Y. Chau and Susanne Brummelte and Joanne Weinberg and Pascal M. Lavoie and Mihoko Ladd and Aaron F. Hirschfeld and Evan Russell and Gideon Koren and Stan Van Uum and Rollin Brant and Stuart E. Turvey
DOI: 10.1371/journal.pone.0073926

Response to Comment on "Helios+ and Helios- Cells Coexist within the Natural FOXP3+ T Regulatory Cell Subset in Humans"
The Journal of Immunology
K. G. MacDonald and J. M. Han and M. E. Himmel and Q. Huang and B. Kan and A. I. M. Campbell and P. M. Lavoie and M. K. Levings
DOI: 10.4049/jimmunol.1390019

Functional Genetic Variation in NFKBIA and Susceptibility to Childhood Asthma, Bronchiolitis, and Bronchopulmonary Dysplasia
The Journal of Immunology
S. Ali and A. F. Hirschfeld and M. L. Mayer and E. S. Fortuno and N. Corbett and M. Kaplan and S. Wang and J. Schneiderman and C. D. Fjell and J. Yan and L. Akhabir and F. Aminuddin and N. Marr and T. Lacaze-Masmonteil and R. G. Hegele and A. Becker and M. Chan-Yeung and R. E. W. Hancock and T. R. Kollmann and D. Daley and A. J. Sandford and P. M. Lavoie and S. E. Turvey
DOI: 10.4049/jimmunol.1201015

Neonatal Sepsis due to Coagulase-Negative Staphylococci
Clinical and Developmental Immunology
Elizabeth A. Marchant and Guilaine K. Boyce and Manish Sadarangani and Pascal M. Lavoie
DOI: 10.1155/2013/586076

The developing human preterm neonatal immune system: A case for more research in this area
Clinical Immunology
Ashish Arunkumar Sharma and Roger Jen and Alison Butler and Pascal M. Lavoie
DOI: 10.1016/j.clim.2012.08.006

Inflammasome-Mediated IL-1ß Production in Humans with Cystic Fibrosis
Anthony Tang and Ashish Sharma and Roger Jen and Aaron F. Hirschfeld and Mark A. Chilvers and Pascal M. Lavoie and Stuart E. Turvey
DOI: 10.1371/journal.pone.0037689

Influence of Common Non-Synonymous Toll-like Receptor 4 Polymorphisms on Bronchopulmonary Dysplasia and Prematurity in Human Infants
Pascal M. Lavoie and Mihoko Ladd and Aaron F. Hirschfeld and Johanna Huusko and Mari Mahlman and David P. Speert and Mikko Hallman and Thierry Lacaze-Masmonteil and Stuart E. Turvey
DOI: 10.1371/journal.pone.0031351

Ex vivo purification and characterization of human invariant natural killer T cells
Journal of Immunological Methods
Ashish Arunkumar Sharma and Leon Chew and Mihoko Ladd and Roger Jen and Pascal M. Lavoie
DOI: 10.1016/j.jim.2011.08.002

Early inflammation in the absence of overt infection in preterm neonates exposed to intensive care
Brent A. Chang and Qing Huang and Joanne Quan and Vann Chau and Mihoko Ladd and Eddie Kwan and Deborah E. McFadden and Thierry Lacaze-Masmonteil and Steven P. Miller and Pascal M. Lavoie
DOI: 10.1016/j.cyto.2011.08.028

Ontogeny of Toll-Like Receptor Mediated Cytokine Responses of Human Blood Mononuclear Cells
Nathan P. Corbett and Darren Blimkie and Kevin C. Ho and Bing Cai and Darren P. Sutherland and Arlene Kallos and Juliet Crabtree and Annie Rein-Weston and Pascal M. Lavoie and Stuart E. Turvey and Natalie R. Hawkins and Steven G. Self and Christopher B. Wilson and Adeline M. Hajjar and Edgardo S. Fortuno and Tobias R. Kollmann
DOI: 10.1371/journal.pone.0015041

Inflammatory Response in Preterm Infants Is Induced Early in Life by Oxygen and Modulated by Total Parenteral Nutrition
Pediatric Research
Pascal M Lavoie and Jean-Claude Lavoie and Carla Watson and Thérèse Rouleau and Brent A Chang and Philippe Chessex
DOI: 10.1203/pdr.0b013e3181eb2f18

Natural killer T cells constitutively expressing the interleukin-2 receptor a chain early in life are primed to respond to lower antigenic stimulation
Mihoko Ladd and Ashish Sharma and Qing Huang and Adele Y. Wang and Lixin Xu and Indira Genowati and Megan K. Levings and Pascal M. Lavoie
DOI: 10.1111/j.1365-2567.2010.03304.x

Genetics of bronchopulmonary dysplasia in the age of genomics
Current Opinion in Pediatrics
Pascal M Lavoie and Marie-Pierre Dubé
DOI: 10.1097/mop.0b013e328336eb85

Incidence of septicemia immediately after elective gastrointestinal contrast procedures in infants: a cohort study
Journal of Pediatric Surgery
Ziad Abu-Sharar and Ashley Robinson and Pascal M. Lavoie
DOI: 10.1016/j.jpedsurg.2009.07.023

Constitutive Expression of CD25 on the Surface of Neonatal (invariant) Natural Killer T Cells Primes Them to Proliferate with Lower Antigenic Stimulation
Clinical Immunology
Ashish Sharma and Mihoko Whalen and Qing Huang and Adele Wang and Lixin Xu and Indira Genowati and Meghan Levings and Pascal Lavoie
DOI: 10.1016/j.clim.2010.03.151

Do neonatologists limit parental decision-making authority? A Canadian perspective
Early Human Development
Susan G. Albersheim and Pascal M. Lavoie and Yaron D. Keidar
DOI: 10.1016/j.earlhumdev.2010.09.007

Profound Lack of Interleukin (IL)–12/IL-23p40 in Neonates Born Early in Gestation Is Associated with an Increased Risk of Sepsis
The Journal of Infectious Diseases
Pascal M. Lavoie and Qing Huang and Elyse Jolette and Mihoko Whalen and Anne Monique Nuyt and Francois Audibert and David P. Speert and Thierry Lacaze-Masmonteil and Hugo Soudeyns and Tobias R. Kollmann
DOI: 10.1086/657143

Neonatal Innate TLR-Mediated Responses Are Distinct from Those of Adults
The Journal of Immunology
T. R. Kollmann and J. Crabtree and A. Rein-Weston and D. Blimkie and F. Thommai and X. Y. Wang and P. M. Lavoie and J. Furlong and E. S. Fortuno and A. M. Hajjar and N. R. Hawkins and S. G. Self and C. B. Wilson
DOI: 10.4049/jimmunol.0901481

Earlier initiation of enteral nutrition is associated with lower risk of late-onset bacteremia only in most mature very low birth weight infants
Journal of Perinatology
P M Lavoie
DOI: 10.1038/jp.2009.8

Heritability of Bronchopulmonary Dysplasia, Defined According to the Consensus Statement of the National Institutes of Health
P. M. Lavoie and C. Pham and K. L. Jang
DOI: 10.1542/peds.2007-2313

Gastrointestinal Presentation of Relative Adrenal Insufficiency in a Sick Preterm Neonate
American Journal of Perinatology
Pascal Lavoie and Gustavo Pelligra and Brian Lupton and Horacio Osiovich
DOI: 10.1055/s-2007-986694

Attitudes of Canadian Neonatologists in Delivery Room Resuscitation of Newborns at Threshold of Viability
Journal of Obstetrics and Gynaecology Canada
Pascal M. Lavoie and Yaron Keidar and Susan Albersheim
DOI: 10.1016/s1701-2163(16)32599-3

Delayed expansion of a restricted T cell repertoire by low-density TCR ligands
International Immunology
Pascal M. Lavoie and Alain R. Dumont and Helen McGrath and Anne-Elen Kernaleguen and Rafick-P. Sékaly
DOI: 10.1093/intimm/dxh273

Lead poisoning from "lead-free" paint
Canadian Medical Association Journal
P. M. Lavoie
DOI: 10.1503/cmaj.1031524

Quantitative Relationship Between MHC Class II-Superantigen Complexes and the Balance of T Cell Activation Versus Death
The Journal of Immunology
P. M. Lavoie and H. McGrath and N. H. Shoukry and P.-A. Cazenave and R.-P. Sekaly and J. Thibodeau
DOI: 10.4049/jimmunol.166.12.7229

Superantigen presenting capacity of human astrocytes
Journal of Neuroimmunology
Mina Hassan-Zahraee and Uma Ladiwala and Pascal M Lavoie and Ellie McCrea and Rafick P Sekaly and Trevor Owens and Jack P Antel
DOI: 10.1016/s0165-5728(99)00174-5

Understanding the mechanism of action of bacterial superantigens from a decade of research
Immunological Reviews
Pascal M. Lavoie and Jacques Thibodeau and Francois Erard and Rafick-Pierre Sekaly
DOI: 10.1111/j.1600-065x.1999.tb01297.x

Role of the T Cell Receptor a Chain in Stabilizing TCR-Superantigen-MHC Class II Complexes
Peter S Andersen and Pascal M Lavoie and Rafick-Pierre Sékaly and Hywyn Churchill and David M Kranz and Patrick M Schlievert and Klaus Karjalainen and Roy A Mariuzza
DOI: 10.1016/s1074-7613(00)80047-3

Viewpoint Conserved structural features between HLA-DOß and -DRß
Molecular Immunology
J Thibodeau and P.M Lavoie and A Samaan and J.-P Corre and R.-P Sékaly and P.-a Cazenave
DOI: 10.1016/s0161-5890(98)00061-3

A Mutational Analysis of the Binding of Staphylococcal Enterotoxins B and C3 to the T Cell Receptor ß Chain and Major Histocompatibility Complex Class II
The Journal of Experimental Medicine
Lukas Leder and Andrea Llera and Pascal M. Lavoie and Marina I. Lebedeva and Hongmin Li and Rafick-Pierre Sékaly and Gregory A. Bohach and Pamala J. Gahr and Patrick M. Schlievert and Klaus Karjalainen and Roy A. Mariuzza
DOI: 10.1084/jem.187.6.823

L'infection par le VIH : importance des facteurs de l'hôte.
P Champagne and PM Lavoie and RP Sékaly and A Yachou
DOI: 10.4267/10608/1001

Selective binding of bacterial toxins to major histocompatibility complex class II-expressing cells is controlled by invariant chain and HLA-DM
Proceedings of the National Academy of Sciences
P. M. Lavoie and J. Thibodeau and I. Cloutier and R. Busch and R.-P. Sekaly
DOI: 10.1073/pnas.94.13.6892

MHC Class II-Dependent Peptide Antigen Versus Superantigen Presentation to T Cells
Human Immunology
Naglaa H Shoukry and Pascal M Lavoie and Jacques Thibodeau and Sushila D’Souza and Rafick P Sekaly
DOI: 10.1016/s0198-8859(97)00074-8

“Bazinc” instinct: how SEA attracts MHC class II molecules
Research in Immunology
J. Thibodeau and P.M. Lavoie and P.-A. Cazenave
DOI: 10.1016/s0923-2494(97)80864-4

Molecular characterization and role in T cell activation of staphylococcal enterotoxin A binding to the HLA-DR alpha-chain.
The Journal of Immunology
Thibodeau, J and Dohlsten, M and Cloutier, I and Lavoie, P M and Bjork, P and Michel, F and L{\'e}veill{\'e}, C and Mourad, W and Kalland, T and S{\'e}kaly, R P}, title= {Molecular characterization and role in T cell activation of staphylococcal enterotoxin A binding to the HLA-DR alpha-chain.}, volume= {158}, number= {8}, pages= {3698--3704}, year= {1997}, publisher= {American Association of Immunologists}, abstract= {Superantigens bind to MHC class II-positive cells and stimulate T lymphocytes expressing specific V beta regions of the TCR. Two distinct regions of staphylococcal enterotoxin A superantigen (SEA) have been shown to affect the binding to MHC class II molecules. Results presented here demonstrate for the first time that the SEA-DR interaction can be affected by mutations on the class II alpha-chain. Furthermore, we have precisely mapped the interaction of the SEA N-terminal domain with the alpha1 domain of HLA-DR. Scatchard analysis using DAP cells transfected with mutant class II molecules showed a role for residue DR alpha K39 in the binding of SEA. Also, complementation experiments using mutant SEA molecules revealed an interaction between SEA residue F47 and position alphaQ18 on an outer loop of HLA-DR. These interactions between SEAF47 and the DR alpha-chain are critical, as they allow the recognition by an otherwise nonreactive V beta1+ T cell hybridoma and induction of tyrosine phosphorylation through the TCR.}, issn= {0022-1767}, URL= {http://www.jimmunol.org/content/158/8/3698}, eprint= {http://www.jimmunol.org/content/158/8/3698.full.pdf}, journal= {The Journal of Immunology

Subsets of HLA-DR1 molecules defined by SEB and TSST-1 binding
J Thibodeau and I Cloutier and P. Lavoie and N Labrecque and W Mourad and T Jardetzky and R. Sekaly
DOI: 10.1126/science.7997881


Anti-inflamatory treatment for bronchopulmonary dysplasia
We are one of the leading centres of the CIHR-funded MOBYDICK Canadian multi-centre randomized clinical trial to determine whether maternal omega-3 supplementation effectively reduces bronchopulmonary dysplasia in very preterm infants.

Heritability of bronchopulmonary dysplasia
Bronchopulmonary dysplasia (BPD) is a form of chronic inflammatory-mediated lung disease affecting thousands of infants born prematurely each year in Canada. BPD is a serious disease which often results in significant morbidity and mortality. Clinicians lack a cure for BPD and current treatments can have serious side-effects. Evidence suggests that susceptibility to this condition is in part genetically inherited. In this study, we collaborate with other neonatal centres internationally to examine the role of common gene polymorphisms in clinically well-characterized cohorts of preterm infants, in order to understand how genetic factors interact in the regulation of inflammation and how they predispose infants to BPD and neonatal conditions more generally.

Innate and adaptive immune defences in human neonates across development
We aim to define characteristics of innate and adaptive immune system in newborns as well as preterm infants that sometimes fail to protect them against infection and inflammatory-mediated neonatal diseases. We study adaptive T cell function in the context of vaccine responses. We develop small scaled immunoassays to characterize the immunological phenotype of these infants from 24 weeks to the term of gestation. We employ molecular and human cell-based immunology approaches (advanced flow cytometry). Our current focus is on the Toll-like receptor/inflammasome innate immune pathways as well as neonatal T cell differentiation.


CIHR Operating Grant – Project: "Maternal Omega-3 supplementation to reduce Bronchopulmonary Dysplasia in very Preterm Infants : A Randomized Controlled Trial (MOBYDIck Trial)" (2014-2019)

CIHR Operating Grant – Project: "Understanding the immunological basis for early-life infections in preterm infants" (2012-2017)

Honours & Awards

CFRI Clinician-Scientist Award – 2008-2013

Michael Smith Foundation for Health Research – Career Investigator Award – 2011-2019

Research Group Members

Maye Cheng, Co-op Student
Elizabeth Marchant, PhD Candidate, Lab manager
Christina Michalski, Doctoral Student
Constantin Radu Popescu, Doctoral Student
Hamid Reza Razzaghian, Postdoctoral Fellow
Zohreh Sharafian, Research Assistant