Overview

Dr. Goldfarb’s current research focuses primarily on novel diagnostic,management and prevention strategies for pediatric infectious disease in resource limited settings.

Publications

Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes.
Scientific reports
Gadkar VJ, Goldfarb DM, Gantt S, Tilley PAG
DOI: 10.1038/s41598-018-23930-1
PubMed: 29615801
04/2018

Evaluation of the Influence of Gastrointestinal Coinfections on Rotavirus Vaccine Effectiveness in Botswana
The Pediatric Infectious Disease Journal
Margaret Mokomane, Jacqueline E. Tate, Andrew P. Steenhoff, Mathew D. Esona, Michael D. Bowen, Kwana Lechiile, Jeffrey M. Pernica, Ishmael Kasvosve, Umesh D. Parashar, David M. Goldfarb
DOI: 10.1097/inf.0000000000001828
03/2018

The global burden of paediatric and neonatal sepsis: a systematic review.
The Lancet. Respiratory medicine
Fleischmann-Struzek C, Goldfarb DM, Schlattmann P, Schlapbach LJ, Reinhart K, Kissoon N
DOI: 10.1016/s2213-2600(18)30063-8
PubMed: 29508706
03/2018

The global problem of childhood diarrhoeal diseases: emerging strategies in prevention and management.
Therapeutic advances in infectious disease
Mokomane M, Kasvosve I, de Melo E, Pernica JM, Goldfarb DM
DOI: 10.1177/2049936117744429
PubMed: 29344358
01/2018

Phylogenetic analyses and antimicrobial resistance profiles of Campylobacter spp. from diarrhoeal patients and chickens in Botswana.
PloS one
de Vries SPW, Vurayai M, Holmes M, Gupta S, Bateman M, Goldfarb D, Maskell DJ, Matsheka MI, Grant AJ
DOI: 10.1371/journal.pone.0194481
PubMed: 29561903
2018

Correction: Rapid enteric testing to permit targeted antimicrobial therapy, with and without Lactobacillus reuteri probiotics, for paediatric acute diarrhoeal disease in Botswana: A pilot, randomized, factorial, controlled trial.
PloS one
Pernica JM, Steenhoff AP, Mokomane M, Moorad B, Lechiile K, Smieja M, Mazhani L, Cheng J, Kelly MS, Loeb M, Stordal K, Goldfarb DM
DOI: 10.1371/journal.pone.0194957
PubMed: 29561910
2018

In reference to should infants who fail their newborn hearing screen undergo cytomegalovirus testing?
The Laryngoscope
Gantt S, Goldfarb DM, Dionne F, Bulman D, Doutré SM
DOI: 10.1002/lary.27032
PubMed: 29266275
12/2017

Rapid enteric testing to permit targeted antimicrobial therapy, with and without Lactobacillus reuteri probiotics, for paediatric acute diarrhoeal disease in Botswana: A pilot, randomized, factorial, controlled trial.
PloS one
Pernica JM, Steenhoff AP, Mokomane M, Moorad B, Lechiile K, Smieja M, Mazhani L, Cheng J, Kelly MS, Loeb M, Stordal K, Goldfarb DM
DOI: 10.1371/journal.pone.0185177
PubMed: 28991918
2017

Pneumococcal Colonization and the Nasopharyngeal Microbiota of Children in Botswana
Open Forum Infectious Diseases
Matthew Kelly, Michael Surette, Marek Smieja, Laura Rossi, Kathy Luinstra, Andrew Steenhoff, David Goldfarb, Tonya Arscott-Mills, Sefelani Boiditswe, Ikanyeng Rulaganyang, Charles Muthoga, Kwana Lechiile, Tiny Mazhani, John Rawls, Coleen Cunningham, Samir Shah, Kristen Feemster, Patrick Seed
DOI: 10.1093/ofid/ofx163.490
2017

Hospital-acquired gastroenteritis at a referral hospital in Gaborone, Botswana
International Journal of Infection Control
Henry Welch, David M. Goldfarb, Banno Moorad, Margaret Mokomane, Marek Smieja, Unoda Chakalisa, Andrew P Steenhoff, Rodney Finalle, Susan E Coffin
DOI: 10.3396/ijic.v13i2.006.17
2017

The Nasopharyngeal Microbiota of Children with Respiratory Infections in Botswana
Pediatric Infectious Disease Journal
Kelly, M.S. and Surette, M.G. and Smieja, M. and Pernica, J.M. and Rossi, L. and Luinstra, K. and Steenhoff, A.P. and Feemster, K.A. and Goldfarb, D.M. and Arscott-Mills, T. and Boiditswe, S. and Rulaganyang, I. and Muthoga, C. and Gaofiwe, L. and Mazhani, T. and Rawls, J.F. and Cunningham, C.K. and Shah, S.S. and Seed, P.C.
DOI: 10.1097/INF.0000000000001607
2017

A comparison of flocked swabs and traditional swabs, using multiplex real-time PCR for detection of common gastroenteritis pathogens in Botswana
Diagnostic Microbiology and Infectious Disease
Mokomane, M. and Kasvosve, I. and Gaseitsiwe, S. and Steenhoff, A.P. and Pernica, J.M. and Lechiile, K. and Luinstra, K. and Smieja, M. and Goldfarb, D.M.
DOI: 10.1016/j.diagmicrobio.2016.07.007
2016

Impact of Rotavirus Vaccination on Hospitalizations and Deaths from Childhood Gastroenteritis in Botswana
Clinical Infectious Diseases
Enane, L.A., Gastañaduy, P.A., Goldfarb, D.M., Pernica, J.M., Mokomane, M., Moorad, B., Masole, L., Tate, J.E., Parashar, U.D., Steenhoff, A.P.
DOI: 10.1093/cid/civ1210
2016

Correlation of clinical outcomes with multiplex molecular testing of stool from children admitted to hospital with gastroenteritis in Botswana
Journal of the Pediatric Infectious Diseases Society
Pernica, J.M. and Steenhoff, A.P. and Welch, H. and Mokomane, M. and Quaye, I. and Arscott-Mills, T. and Mazhani, L. and Lechiile, K. and Mahony, J. and Smieja, M. and Goldfarb, D.M.
DOI: 10.1093/jpids/piv028
2016

Effectiveness of Monovalent Rotavirus Vaccine after Programmatic Implementation in Botswana: A Multisite Prospective Case-Control Study
Clinical Infectious Diseases
Gastañaduy, P.A., Steenhoff, A.P., Mokomane, M., Esona, M.D., Bowen, M.D., Jibril, H., Pernica, J.M., Mazhani, L., Smieja, M., Tate, J.E., Parashar, U.D., Goldfarb, D.M.
DOI: 10.1093/cid/civ1207
2016

Cost-effectiveness of universal and targeted newborn screening for congenital cytomegalovirus infection
JAMA Pediatrics
Gantt, S. and Dionne, F. and Kozak, F.K. and Goshen, O. and Goldfarb, D.M. and Park, A.H. and Boppana, S.B. and Fowler, K.
DOI: 10.1001/jamapediatrics.2016.2016
2016

Enteric Parasites in Arctic Communities: Tip of the Iceberg?
Trends in Parasitology
Yansouni, C.P. and Pernica, J.M. and Goldfarb, D.
DOI: 10.1016/j.pt.2016.08.004
2016

Molecular Detection of Diarrheal Pathogens
Clinical Microbiology Newsletter
Smieja, M. and Goldfarb, D.M.
DOI: 10.1016/j.clinmicnews.2016.08.001
2016

Bordetella species other than bordetella pertussis
Clinical Microbiology Newsletter
Srigley, J.A. and Goldfarb, D.M. and Pernica, J.M.
DOI: 10.1016/j.clinmicnews.2015.03.004
2015

Optimizing Management of Pediatric Diarrhoeal Disease in Botswana: A Pilot, Factorial, Randomized, Placebo-Controlled Trial of Rapid Enteric Diagnostics and Probiotic Therapy
Open Forum Infectious Diseases
Jeffrey Pernica, Andrew Steenhoff, Margaret Mokomane, Kwana Lechiile, Banno Moorad, Marek Smieja, Loeto Mazhani, Mark Loeb, Ketil Stordal, David Goldfarb
DOI: 10.1093/ofid/ofv133.1177
2015

Association of respiratory viruses with outcomes of severe childhood pneumonia in Botswana
PLoS ONE
Kelly, M.S. and Smieja, M. and Luinstra, K. and Wirth, K.E. and Goldfarb, D.M. and Steenhoff, A.P. and Arscott-Mills, T. and Cunningham, C.K. and Boiditswe, S. and Sethomo, W. and Shah, S.S. and Finalle, R. and Feemster, K.A.
DOI: 10.1371/journal.pone.0126593
2015

Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in diarrhoeic patients in the Qikiqtani Region, Nunavut, Canada
International Journal of Circumpolar Health
Iqbal, A. and Goldfarb, D.M. and Slinger, R. and Dixon, B.R.
DOI: 10.3402/ijch.v74.27713
2015

Risk factors for measles in HIV-infected children and adolescents in Botswana
Pediatric Infectious Disease Journal
Wirth, K.E. and Wolf, E.R. and Goldfarb, D.M. and Ho-Foster, A. and Tolle, M. and Jacovides, C. and Kirk, B. and Chise, M. and Steenhoff, A.P.
DOI: 10.1097/INF.0000000000000816
2015

Early childhood diarrhoeal diseases and cognition: Are we missing the rest of the iceberg?
Paediatrics and International Child Health
Macintyre, J. and McTaggart, J. and Guerrant, R.L. and Goldfarb, D.M.
DOI: 10.1179/2046905514Y.0000000141
2014

Evaluation of anatomically designed flocked rectal swabs for molecular detection of enteric pathogens in children admitted to hospital with severe gastroenteritis in botswana
Journal of Clinical Microbiology
Goldfarb, D.M. and Steenhoff, A.P. and Pernica, J.M. and Chong, S. and Luinstra, K. and Mokomane, M. and Mazhani, L. and Quaye, I. and Goercke, I. and Mahony, J. and Smieja, M.
DOI: 10.1128/JCM.01894-14
2014

CT before lumbar puncture in suspected meningitis in Botswana: How established guidelines may not apply
African Journal of Emergency Medicine
Kestler, A. and Caruso, N. and Chandra, A. and Goldfarb, D. and Haas, M.
DOI: 10.1016/j.afjem.2013.07.004
2014

Hospital-Based Surveillance for Rotavirus Gastroenteritis using Molecular Testing and Immunoassay During the 2011 Season in Botswana
The Pediatric Infectious Disease Journal
Henry Welch, Andrew P. Steenhoff, Unoda Chakalisa, Tonya Arscott-Mills, Loeto Mazhani, Margaret Mokomane, Sara Foster-Fabiano, Kathleen E. Wirth, Andrew Skinn, Jeffrey M. Pernica, Marek Smieja, David M. Goldfarb
DOI: 10.1097/inf.0b013e3182847295
05/2013

Microbiology of Urinary Tract Infections in Gaborone, Botswana
PLoS ONE
Andrew J. Renuart, David M. Goldfarb, Margaret Mokomane, Ephraim O. Tawanana, Mohan Narasimhamurthy, Andrew P. Steenhoff, Jonathan A. Silverman
DOI: 10.1371/journal.pone.0057776
03/2013

Yield of contact tracing from pediatric tuberculosis index cases in Gaborone, Botswana
International Journal of Tuberculosis and Lung Disease
Puryear, S. and Seropola, G. and Ho-Foster, A. and Arscott-Mills, T. and Mazhani, L. and Firth, J. and Goldfarb, D.M. and Ncube, R. and Bisson, G.P. and Steenhoff, A.P.
DOI: 10.5588/ijtld.12.0933
2013

Development and evaluation of global child health educational modules
Pediatrics
Audcent, T.A. and MacDonnell, H.M. and Moreau, K.A. and Hawkes, M. and Sauve, L.J. and Crockett, M. and Fisher, J.A. and Goldfarb, D.M. and Hunter, A.J. and McCarthy, A.E. and Pernica, J.M. and Liu, J. and Luong, T.-N. and Sandhu, A.K. and Rashed, S. and Levy, A. and Brenner, J.L.
DOI: 10.1542/peds.2013-1534
2013

Nanolitre real-time PCR detection of bacterial, parasitic, and viral agents from patients with diarrhoea in Nunavut, Canada
International Journal of Circumpolar Health
Goldfarb, D.M. and Dixon, B. and Moldovan, I. and Barrowman, N. and Mattison, K. and Zentner, C. and Baikie, M. and Bidawid, S. and Chan, F. and Slinger, R.
DOI: 10.3402/ijch.v72i0.19903
2013

Hospital-based surveillance for rotavirus gastroenteritis using molecular testing and immunoassay during the 2011 season in Botswana
Pediatric Infectious Disease Journal
Welch, H. and Steenhoff, A.P. and Chakalisa, U. and Arscott-Mills, T. and Mazhani, L. and Mokomane, M. and Foster-Fabiano, S. and Wirth, K.E. and Skinn, A. and Pernica, J.M. and Smieja, M. and Goldfarb, D.
DOI: 10.1097/INF.0b013e3182847295
2013

Severe late-onset multisystem cytomegalovirus infection in a premature neonate previously treated for congenital infection
BMC Pediatrics
El-Sayed, M.F. and Goldfarb, D.M. and Fulford, M. and Pernica, J.M.
DOI: 10.1186/1471-2431-13-142
2013

Rapid PCR detection of group a streptococcus from flocked throat swabs: A retrospective clinical study
Annals of Clinical Microbiology and Antimicrobials
Slinger, R. and Goldfarb, D. and Rajakumar, D. and Moldovan, I. and Barrowman, N. and Tam, R. and Chan, F.
DOI: 10.1186/1476-0711-10-33
2011

Prevalence of methicillin-resistant Staphylococcus aureus colonization in children and adolescents admitted to CHEO
Paediatrics and Child Health
Pernica, J.M. and Grzyb, M. and Goldfarb, D.M. and Slinger, R. and Suh, K.
2010

Protocol for management of imported pediatric malaria decreases time to medication administration
Pediatric Infectious Disease Journal
Goldfarb, D.M. and Gaboury, I. and Dayneka, N. and Saux, N.L.
DOI: 10.1097/INF.0b013e3181a3d12f
2009

Assessment of flocked swabs for use in identification of streptococcal pharyngitis
Journal of Clinical Microbiology
Goldfarb, D.M. and Slinger, R. and Tam, R.K. and Barrowman, N. and Chan, F.
DOI: 10.1128/JCM.01163-09
2009

Index of suspicion.
Pediatrics in review / American Academy of Pediatrics
Liu, H. and Patel, R. and Lee, B. and Pernica, J.M. and Goldfarb, D. and Harrison, M.E.
2009

Detection of plasmid-mediated KPC-producing Klebsiella pneumoniae in Ottawa, Canada: Evidence of intrahospital transmission
Journal of Clinical Microbiology
Goldfarb, D. and Harvey, S.-B. and Jessamine, K. and Jessamine, P. and Toye, B. and Desjardins, M.
DOI: 10.1128/JCM.00098-09
2009

Seasonal timing of the Royal College examination in general paediatrics: A survey of residents' preferences
Paediatrics and Child Health
Brill, H. and Benchimol, E.I. and Goldfarb, D.
2007

Case 2: Newborn with jaundice and "hyperglycemia"
Paediatrics and Child Health
Goldfarb, D. and Chakraborty, P.
2006

Research

Gastrointestinal Infections in the Arctic
Nunavut has an unusually high infection rate of cryptosporidium, a parasite that causes abdominal cramps and diarrhea. Children in the region are particularly impacted by these infections. When coupled with malnutrition, the parasite could affect early brain development. I am involved in a registry aimed at documenting enteric infections in the Canadian Arctic and am also leading a prospective study to investigate outcomes of pediatric cryptosporidium infections in Northern communities.

Improving Neonatal Care in Malawi
Malawi has the highest preterm delivery rate (18%) in the world. Inadequate newborn care at the facility level, particularly care of preterm infants, has resulted in an unacceptably high neonatal mortality rate in Malawi. I am a co-principal investigator on a project aimed at implementing various simple, life-saving, evidence-based interventions into routine clinical practice at Malawi hospitals.We hope that our program will result in reduced neonatal mortality and that our knowledge can be applied to other sub-saharan countries.

Point of Care Testing of Gastroenteritis in Botswana:
I am leading a team of collaborators from the University of Pennsylvania, University of Botswana and McMaster that plans to design an innovative, user- and patient-friendly sample collection method that will allow for point of care testing for gastroenteritis. Combined with more recent, highly sensitive detection techniques, this work could have immediate impact in much of the world where diarrheal disease remains deadly and for the large part undiagnosed.

Grants

2015 Apr - 2018 Mar. Yansouni, C (PI), Goldfarb, DM (co-PI), Diarrheal Illness and Enteric Infections Among Young Children in Nunavik and Nunavut, ArcticNet PhaseIV, Research - $120,000 (awarded)

2015 Apr - 2020 Mar. Dube, Q (PI), Goldfarb, DM (co-PI), Integrating a Neonatal Health Care Package in Malawi, International Development Research Centre/CIHR - $1,000,000 (awarded)

2015 Jan - 2016 Jan. Goldfarb DM (PI), Building an Arctic Enteric Infections Research Agenda. Canadian Institutes of Health Research Planning and Dissemination Grant, $12,000

Honours & Awards

2008 University of Ottawa, Dept of Pathology & Lab Medicine Annual Research Day - 2nd prize

2008 Children’s Hospital of Eastern Ontario, Popham Fellowship

2008 Children’s Hospital of Eastern Ontario Research Institute, Best Sub-specialty Resident Presentation

Research Group Members

Margaret Mokomane, Doctoral student
Moses Vurayai, Master's student
Emilia de Melo, Master's student
Bronwyn Barker
Haya Abuzuluf