Overview

Research in my laboratory is focused on a novel subset of CD4+ T cells, termed T regulatory (Treg) cells, which control immune homeostasis. Although we know that Treg cells have a fundamental role in regulating immunity to both self and foreign proteins, little is known about how they function. Current work is focused on determining how Treg cells differ from normal CD4+ T cells at both the biochemical and molecular levels, and elucidating their role in transplantation tolerance, cancer and inflammatory bowel disease. A long-term goal is to develop methods to generate Treg cells in vitro for use as a cellular therapy to replace standard immunosuppression in the context of organ transplantation or to restore tolerance in the context of autoimmunity.

Publications

Megadose Bone Marrow and Regulatory T cells for the Induction of Immune Tolerance in Non-Human Primates Through Durable Mixed Hematopoietic Chimerism Across MHC-barriers
Transplantation
Paula Alonso-Guallart and Raimon Duran-Struuck and Jeffrey Stern and Erik Berglund and Nathaly Llore and Jonah Zitsman and Genevieve Pierre and Sigal Kofman and Makenzie Danton and Samanta Gokova and Emily Lopes and Alicia McMurchy and Qizhi Tang and Megan Levings and Megan Sykes and Adam Griesemer
DOI: 10.1097/01.tp.0000542954.80179.6b
07/2018

Engineering therapeutic T cells to suppress alloimmune responses using TCRs, CARs, or BARs
American Journal of Transplantation
Antoine Sicard and Megan K. Levings and David W. Scott
DOI: 10.1111/ajt.14747
04/2018

Guiding regulatory T cells to the allograft
Current Opinion in Organ Transplantation
Caroline Lamarche and Megan K. Levings
DOI: 10.1097/mot.0000000000000483
11/2017

Engineered Tolerance: Tailoring Development, Function, and Antigen-Specificity of Regulatory T Cells
Frontiers in Immunology
Nicholas A. J. Dawson and Jens Vent-Schmidt and Megan K. Levings
DOI: 10.3389/fimmu.2017.01460
11/2017

Harnessing Advances in T Regulatory Cell Biology for Cellular Therapy in Transplantation
Transplantation
Avery J. Lam and Romy E. Hoeppli and Megan K. Levings
DOI: 10.1097/tp.0000000000001757
10/2017

Antigen-specific regulatory T cells: are police CARs the answer?
Translational Research
Nicholas A.J. Dawson and Megan K. Levings
DOI: 10.1016/j.trsl.2017.06.009
09/2017

The outstanding questions in transplantation: It's about time…
American Journal of Transplantation
Jamil Azzi and Giorgio Raimondi and Valeria Mas and Leonardo V. Riella and Nissreen Elfadawy and Kassem Safa and David Wojciechowski and Mazhar Kanak and Rajat Nog and Jonathan S. Maltzman and Mandy L. Ford and Jordan S. Pober and Xung-Rong Luo and David Rothstein and Michelle L. Miller and David Matthews and William Burlingham and Megan Levings and Peter Heeger and Lauren Higdon and John Gill and Ronald G. Gill and Maria-Luisa Alegre
DOI: 10.1111/ajt.14450
09/2017

Adipose-tissue regulatory T cells: Critical players in adipose-immune crosstalk
European Journal of Immunology
Maike Becker and Megan K. Levings and Carolin Daniel
DOI: 10.1002/eji.201646739
09/2017

OR42 Expanded thymic regulatory T cells (Tregs) have low class I HLA expression levels and are HLA-DR negative – Potential for ‘Off-The-Shelf’ Cellular Therapy?
Human Immunology
Esme Dijke and Romy Hoeppli and Ingrid Larsen and Ivan Rebeyka and David Ross and Megan Levings and Lori West
DOI: 10.1016/j.humimm.2017.06.048
09/2017

Regulatory T-cells drive immune dysfunction in CLL
Leukemia & Lymphoma
Deepesh Lad and Romy Hoeppli and Qing Huang and Rosa Garcia and Lixin Xu and Cynthia Toze and Raewyn Broady and Megan Levings
DOI: 10.1080/10428194.2017.1330475
06/2017

Thymic progenitors of TCRaß + CD8aa intestinal intraepithelial lymphocytes require RasGRP1 for development
The Journal of Experimental Medicine
Dominic P. Golec and Romy E. Hoeppli and Laura M. Henao Caviedes and Jillian McCann and Megan K. Levings and Troy A. Baldwin
DOI: 10.1084/jem.20170844
06/2017

Migration Capacity of Thymic Regulatory T Cells can be Tuned by Expansion in Cytokine-Enriched Culture Conditions
Transplantation
Romy E. Hoeppli and Esmé Dijke and Andrew Campbell and Lori J. West and Megan K. Levings
DOI: 10.1097/01.tp.0000520352.47820.13
05/2017

Standardized Immunophenotyping in the Canadian National Transplant Research Program
Transplantation
Sabine Ivison and Salima Janmohamed and Mehrnoush Malek and Rosa Garcia and Raewyn Broady and Anne Halpin and Manon Richaud and Rollin Brant and Jean-Sebastien Delisle and Lori West and Ryan R. Brinkman and Megan Levings
DOI: 10.1097/01.tp.0000520396.84839.9b
05/2017

Thymus-Derived Treg Infusion to Prevent Graft Rejection in Heart-Transplanted Children
Transplantation
Esther Bernaldo de Quirós and Verónica A. Pérez and Maribel Clemente and Juan Miguel Gil-Jaurena and María Teresa González and Marjorie Pion and Giovanna Lombardi and Lori J. West and Megan Levings and Rafael Correa-Rocha
DOI: 10.1097/01.tp.0000520350.40197.6b
05/2017

Development of GMP-Compatible Protocols for Thymus-Derived Regulatory T Cell Expansion
Transplantation
Katherine MacDonald and Romy Hoeppli and James Piret and Esmé Dijke and Lori West and Megan Levings
DOI: 10.1097/01.tp.0000520303.10595.5e
05/2017

A Novel Whole Blood Assay Detects Flagellin-Specific CD4 + T Cells in Patients with Inflammatory Bowel Disease
Gastroenterology
Laura Cook and May Q. Wong and Rosa Garcia and Megan E. Himmel and Brian Bressler and Ernest G. Seidman and Theodore S. Steiner and Megan K. Levings
DOI: 10.1016/s0016-5085(17)32185-6
04/2017

Evaluating the Role of Tregs in the Progression of Multiple Myeloma
Clinical Lymphoma Myeloma and Leukemia
Deeepesh Lad and Raewyn Broady and Jessica Huang and Romy Hoeppli and Anne Pesenacker and Rosa Garcia and Lixin Xu and Megan Levings and Kevin Song
DOI: 10.1016/j.clml.2017.03.075
02/2017

Effect of Ex Vivo–Expanded Recipient Regulatory T Cells on Hematopoietic Chimerism and Kidney Allograft Tolerance Across MHC Barriers in Cynomolgus Macaques
Transplantation
Raimon Duran-Struuck and Hugo P. Sondermeijer and Leo Bühler and Paula Alonso-Guallart and Jonah Zitsman and Yojiro Kato and Anette Wu and Alicia N. McMurchy and David Woodland and Adam Griesemer and Mercedes Martinez and Svetlan Boskovic and Tatsuo Kawai and A. Benedict Cosimi and Cheng-Shie Wuu and Andrea Slate and Markus Y. Mapara and Sam Baker and Rafal Tokarz and Vivette D'Agati and Scott Hammer and Marcus Pereira and W. Ian Lipkin and Thomas Wekerle and Megan K. Levings and Megan Sykes
DOI: 10.1097/tp.0000000000001559
02/2017

CD4+ T Cells Specific for C. difficile Toxins are a Marker of Patients with Active Relapsing Disease
Open Forum Infectious Diseases
Laura Cook and May Wong and William Rees and Torey Lau and Megan Levings and Theodore Steiner
DOI: 10.1093/ofid/ofx163.948
2017

Circulating gluten-specific FOXP3 + CD39 + regulatory T cells have impaired suppressive function in patients with celiac disease
Journal of Allergy and Clinical Immunology
Laura Cook and C. Mee Ling Munier and Nabila Seddiki and David van Bockel and Noé Ontiveros and Melinda Y. Hardy and Jana K. Gillies and Megan K. Levings and Hugh H. Reid and Jan Petersen and Jamie Rossjohn and Robert P. Anderson and John J. Zaunders and Jason A. Tye-Din and Anthony D. Kelleher
DOI: 10.1016/j.jaci.2017.02.015
2017

Biomarker-guided stratification of autoimmune patients for biologic therapy
Current Opinion in Immunology
Sabine Ivison and Christine Des Rosiers and Sylvie Lesage and John D Rioux and Megan K Levings
DOI: 10.1016/j.coi.2017.09.006
2017

Filgrastim-Stimulated Bone Marrow Compared with Filgrastim-Mobilized Peripheral Blood in Myeloablative Sibling Allografting for Patients with Hematologic Malignancies: A Randomized Canadian Blood and Marrow Transplant Group Study
Biology of Blood and Marrow Transplantation
Stephen Couban and Mahmoud Aljurf and Sylvie Lachance and Irwin Walker and Cynthia Toze and Morel Rubinger and Jeffrey H. Lipton and Stephanie J. Lee and Jeff Szer and Richard Doocey and Ian D. Lewis and Lothar Huebsch and Kang Howson-Jan and Michel Lalancette and Fahad Almohareb and Nadeem Chaudhri and Sabine Ivison and Raewyn Broady and Megan Levings and Diane Fairclough and Gerald Devins and David Szwajcer and Ronan Foley and Clayton Smith and Tony Panzarella and Holly Kerr and Amina Kariminia and Kirk R. Schultz
DOI: 10.1016/j.bbmt.2016.04.017
08/2016

How antigen specificity directs regulatory T-cell function: self, foreign and engineered specificity
HLA
R. E. Hoeppli and K. G. MacDonald and M. K. Levings and L. Cook
DOI: 10.1111/tan.12822
06/2016

Alloantigen-specific regulatory T cells generated with a chimeric antigen receptor
Journal of Clinical Investigation
Katherine G. MacDonald and Romy E. Hoeppli and Qing Huang and Jana Gillies and Dan S. Luciani and Paul C. Orban and Raewyn Broady and Megan K. Levings
DOI: 10.1172/jci82771
03/2016

T regulatory cell chemokine production mediates pathogenic T cell attraction and suppression
Journal of Clinical Investigation
Scott J. Patterson and Anne M. Pesenacker and Adele Y. Wang and Jana Gillies and Majid Mojibian and Kim Morishita and Rusung Tan and Timothy J. Kieffer and C. Bruce Verchere and Constadina Panagiotopoulos and Megan K. Levings
DOI: 10.1172/jci83987
02/2016

A Regulatory T-Cell Gene Signature Is a Specific and Sensitive Biomarker to Identify Children With New-Onset Type 1 Diabetes
Diabetes
Anne M. Pesenacker and Adele Y. Wang and Amrit Singh and Jana Gillies and Youngwoong Kim and Ciriaco A. Piccirillo and Duc Nguyen and W. Nicholas Haining and Scott J. Tebbutt and Constadina Panagiotopoulos and Megan K. Levings
DOI: 10.2337/db15-0572
01/2016

Obesity-Associated Adipose Tissue Inflammation and Transplantation
American Journal of Transplantation
D. Wu and N. A. J. Dawson and M. K. Levings
DOI: 10.1111/ajt.13578
01/2016

The role of FOXP3 in autoimmunity
Current Opinion in Immunology
Anne M Pesenacker and Laura Cook and Megan K Levings
DOI: 10.1016/j.coi.2016.07.004
2016

CCL22 Prevents Rejection of Mouse Islet Allografts and Induces Donor-Specific Tolerance
Cell Transplantation
Joel Montane and Merce Obach and Sigrid Alvarez and Loraine Bischoff and Derek L. Dai and Galina Soukhatcheva and John J. Priatel and Gijs Hardenberg and Megan K. Levings and Rusung Tan and Paul C. Orban and C. Bruce Verchere
DOI: 10.3727/096368914x685249
10/2015

Discarded Human Thymus Is a Novel Source of Stable and Long-Lived Therapeutic Regulatory T Cells
American Journal of Transplantation
I. E. Dijke and R. E. Hoeppli and T. Ellis and J. Pearcey and Q. Huang and A. N. McMurchy and K. Boer and A. M. A. Peeters and G. Aubert and I. Larsen and D. B. Ross and I. Rebeyka and A. Campbell and C. C. Baan and M. K. Levings and L. J. West
DOI: 10.1111/ajt.13456
09/2015

Tr1 Cells, but Not Foxp3+Regulatory T Cells, Suppress NLRP3 Inflammasome Activation via an IL-10–Dependent Mechanism
The Journal of Immunology
Yu Yao and Jens Vent-Schmidt and Matthew D. McGeough and May Wong and Hal M. Hoffman and Theodore S. Steiner and Megan K. Levings
DOI: 10.4049/jimmunol.1403225
06/2015

IL-33 Reverses an Obesity-Induced Deficit in Visceral Adipose Tissue ST2+T Regulatory Cells and Ameliorates Adipose Tissue Inflammation and Insulin Resistance
The Journal of Immunology
Jonathan M. Han and Dan Wu and Heather C. Denroche and Yu Yao and C. Bruce Verchere and Megan K. Levings
DOI: 10.4049/jimmunol.1500020
04/2015

Regulatory T cells produce profibrotic cytokines in the skin of patients with systemic sclerosis
Journal of Allergy and Clinical Immunology
Katherine G. MacDonald and Nicholas A.J. Dawson and Qing Huang and James V. Dunne and Megan K. Levings and Raewyn Broady
DOI: 10.1016/j.jaci.2014.12.1932
04/2015

The Environment of Regulatory T Cell Biology: Cytokines, Metabolites, and the Microbiome
Frontiers in Immunology
Romy E. Hoeppli and Dan Wu and Laura Cook and Megan K. Levings
DOI: 10.3389/fimmu.2015.00061
02/2015

Restimulation After Cryopreservation and Thawing Preserves the Phenotype and Function of Expanded Baboon Regulatory T Cells
Transplantation Direct
Joshua Weiner and Raimon Duran-Struuck and Jonah Zitsman and Leo Buhler and Hugo Sondermeijer and Alicia N. McMurchy and Megan K. Levings and Megan Sykes and Adam Griesemer
DOI: 10.1097/txd.0000000000000511
02/2015

Phenotype, Function and Expansion of Regulatory T Cells in the Cynomolgus Macaque (Macaca fascicularis)
Biology of Blood and Marrow Transplantation
Paula Alonso Guallart and Jonah Zitsman and Hugo Sondermeijer and Leo Bühler and Christopher Ovanez and Alicia McMurchy and Megan Levings and Megan Sykes and Raimon Duran-Struuck
DOI: 10.1016/j.bbmt.2014.11.445
02/2015

Induction of Durable Mixed Hematopoietic Chimerism and Immune Tolerance in Monkeys
Biology of Blood and Marrow Transplantation
Paula Alonso Guallart and Jonah Zitsman and Hugo Sondermeijer and David Woodland and Yojiro Kato and Joshua Weiner and Adam Griesemer and Leo Bühler and Alicia McMurchy and Megan Levings and Megan Sykes and Raimon Duran-Struuck
DOI: 10.1016/j.bbmt.2014.11.040
02/2015

Methyltransferase G9A regulates T cell differentiation during murine intestinal inflammation
Journal of Clinical Investigation
Frann Antignano and Kyle Burrows and Michael R. Hughes and Jonathan M. Han and Ken J. Kron and Nadia M. Penrod and Menno J. Oudhoff and Steven Kai Hao Wang and Paul H. Min and Matthew J. Gold and Alistair L. Chenery and Mitchell J.S. Braam and Thomas C. Fung and Fabio M.V. Rossi and Kelly M. McNagny and Cheryl H. Arrowsmith and Mathieu Lupien and Megan K. Levings and Colby Zaph
DOI: 10.1172/jci69592
03/2014

Control of tissue-localized immune responses by human regulatory T cells
European Journal of Immunology
Anne M. Pesenacker and Raewyn Broady and Megan K. Levings
DOI: 10.1002/eji.201344205
2014

The Role of FOXP3 in Regulating Immune Responses
International Reviews of Immunology
Jens Vent-Schmidt and Jonathan M. Han and Katherine G. MacDonald and Megan K. Levings
DOI: 10.3109/08830185.2013.811657
08/2013

Immune Regulation in Obesity-Associated Adipose Inflammation
The Journal of Immunology
J. M. Han and M. K. Levings
DOI: 10.4049/jimmunol.1301035
07/2013

Response to Comment on "Helios+ and Helios- Cells Coexist within the Natural FOXP3+ T Regulatory Cell Subset in Humans"
The Journal of Immunology
K. G. MacDonald and J. M. Han and M. E. Himmel and Q. Huang and B. Kan and A. I. M. Campbell and P. M. Lavoie and M. K. Levings
DOI: 10.4049/jimmunol.1390019
04/2013

Autologous transplant for autoimmune disease: optimizing the regulatory T cells
Cytotherapy
T.D. Prestidge and J.H. Davis and J. Huang and M. Himmel and M. Levings
DOI: 10.1016/j.jcyt.2013.01.161
04/2013

Pediatric thymic tissue as a source of CD25+FOXP3+ regulatory T cells (Tregs) for cellular therapy
Cytotherapy
E. Dijke and M. Levings and A. McMurchy and I. Larsen and I. Rebeyka and D. Ross and L. West
DOI: 10.1016/j.jcyt.2013.01.160
04/2013

Helios+ and Helios- Cells Coexist within the Natural FOXP3+ T Regulatory Cell Subset in Humans
The Journal of Immunology
M. E. Himmel and K. G. MacDonald and R. V. Garcia and T. S. Steiner and M. K. Levings
DOI: 10.4049/jimmunol.1201379
01/2013

Th-2 cytokine producing Tregs in the skin of patients with systemic sclerosis
Frontiers in Immunology
Broady Raewyn and Huang Qing and MacDonald Katherine and Dunne James and Levings Megan
DOI: 10.3389/conf.fimmu.2013.02.01011
2013

Insulin impairs regulatory T cell function: implications for obesity
Frontiers in Immunology
Han Jonathan and Patterson Scott and Ehses Jan and Levings Megan
DOI: 10.3389/conf.fimmu.2013.02.00587
2013

Expansion of Highly Suppressive FOXP3+ Regulatory T Cells from Pediatric Thymic Tissue
Transplantation Journal
E. Dijke and A. McMurchy and M. Levings and I. Larsen and I. Rebeyka and D. Ross and L. West
DOI: 10.1097/00007890-201211271-02368
11/2012

A novel function for FOXP3 in humans: intrinsic regulation of conventional T cells
Blood
A. N. McMurchy and J. Gillies and M. C. Gizzi and M. Riba and J. M. Garcia-Manteiga and D. Cittaro and D. Lazarevic and S. Di Nunzio and I. S. Piras and A. Bulfone and M. G. Roncarolo and E. Stupka and R. Bacchetta and M. K. Levings
DOI: 10.1182/blood-2012-05-431023
11/2012

In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells
Basic Cell Culture Protocols
Alicia N. McMurchy and Megan K. Levings
DOI: 10.1007/978-1-62703-128-8_8
10/2012

Chemokine Induced Tolerance in Mouse Islet Allografts
Canadian Journal of Diabetes
Joel Montane and Merce Obach and Loraine Bischoff and Galina Soukhatcheva and Paul C. Orban and Gijs Hardenberg and Megan K. Levings and Rusung Tan and C. Bruce Verchere
DOI: 10.1016/j.jcjd.2012.07.468
10/2012

ATP conditions intestinal epithelial cells to an inflammatory state that promotes components of DC maturation
European Journal of Immunology
Yu Yao and Megan K. Levings and Theodore S. Steiner
DOI: 10.1002/eji.201142213
10/2012

Active vitamin D (1,25-dihydroxyvitamin D3) increases host susceptibility to Citrobacter rodentium by suppressing mucosal Th17 responses
AJP: Gastrointestinal and Liver Physiology
N. R. Ryz and S. J. Patterson and Y. Zhang and C. Ma and T. Huang and G. Bhinder and X. Wu and J. Chan and A. Glesby and H. P. Sham and J. P. Dutz and M. K. Levings and K. Jacobson and B. A. Vallance
DOI: 10.1152/ajpgi.00320.2012
09/2012

T regulatory cell therapy in transplantation
Current Opinion in Organ Transplantation
Katherine G. MacDonald and Paul C. Orban and Megan K. Levings
DOI: 10.1097/mot.0b013e328355aaaf
08/2012

Regulatory T-cell therapy for inflammatory bowel disease: more questions than answers
Immunology
Megan E. Himmel and Yu Yao and Paul C. Orban and Theodore S. Steiner and Megan K. Levings
DOI: 10.1111/j.1365-2567.2012.03572.x
04/2012

Inflammation-Driven Reprogramming of CD4+Foxp3+ Regulatory T Cells into Pathogenic Th1/Th17 T Effectors Is Abrogated by mTOR Inhibition in vivo
PLoS ONE
Ekaterina Yurchenko and Marina T. Shio and Tony C. Huang and Maria Da Silva Martins and Moshe Szyf and Megan K. Levings and Martin Olivier and Ciriaco A. Piccirillo
DOI: 10.1371/journal.pone.0035572
04/2012

Toll-like receptor 5 deficiency protects from wasting disease in a T cell transfer colitis model in T cell receptor-ß-deficient mice
Inflammatory Bowel Diseases
Gijs Hardenberg and Yu Yao and Ciriaco A. Piccirillo and Megan K. Levings and Theodore S. Steiner
DOI: 10.1002/ibd.21738
01/2012

TH17 Cells in Autoimmunity and Immunodeficiency: Protective or Pathogenic?
Frontiers in Immunology
Ashish K. Marwaha and Nicole J. Leung and Alicia N. McMurchy and Megan K. Levings
DOI: 10.3389/fimmu.2012.00129
2012

Transcriptome Analysis Reveals Markers of Aberrantly Activated Innate Immunity in Vitiligo Lesional and Non-Lesional Skin
PLoS ONE
Richard Yu and Raewyn Broady and Yuanshen Huang and Yang Wang and Jie Yu and Min Gao and Megan Levings and Shencai Wei and Shengquan Zhang and Aie Xu and Mingwan Su and Jan Dutz and Xuejun Zhang and Youwen Zhou
DOI: 10.1371/journal.pone.0051040
2012

The Role of the PI3K Signaling Pathway in CD4+ T Cell Differentiation and Function
Frontiers in Immunology
Jonathan M. Han and Scott J. Patterson and Megan K. Levings
DOI: 10.3389/fimmu.2012.00245
2012

Cellular magnetic resonance imaging of monocyte-derived dendritic cell migration from healthy donors and cancer patients as assessed in a scid mouse model
Cytotherapy
Xizhong Zhang and Sonali N. de Chickera and Christy Willert and Vasliki Economopoulos and Jennifer Noad and Roja Rohani and Adele Y. Wang and Megan K. Levings and Elizabeth Scheid and Ronan Foley and Paula J. Foster and Gregory A. Dekaban
DOI: 10.3109/14653249.2011.605349
11/2011

Human Th1 and Th17 Cells Exhibit Epigenetic Stability at Signature Cytokine and Transcription Factor Loci
The Journal of Immunology
C. J. Cohen and S. Q. Crome and K. G. MacDonald and E. L. Dai and D. L. Mager and M. K. Levings
DOI: 10.4049/jimmunol.1101058
11/2011

CD4+Foxp3+ regulatory T cells suppress ¿d T-cell effector functions in a model of T-cell-induced mucosal inflammation
European Journal of Immunology
Ekaterina Yurchenko and Megan K. Levings and Ciriaco A. Piccirillo
DOI: 10.1002/eji.201141814
11/2011

Moving to tolerance: Clinical application of T regulatory cells
Seminars in Immunology
Alicia N. McMurchy and Andrew Bushell and Megan K. Levings and Kathryn J. Wood
DOI: 10.1016/j.smim.2011.04.001
08/2011

Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets
Journal of Clinical Investigation
Joel Montane and Loraine Bischoff and Galina Soukhatcheva and Derek L. Dai and Gijs Hardenberg and Megan K. Levings and Paul C. Orban and Timothy J. Kieffer and Rusung Tan and C. Bruce Verchere
DOI: 10.1172/jci43048
08/2011

SHIP-Deficient Dendritic Cells, Unlike Wild Type Dendritic Cells, Suppress T Cell Proliferation via a Nitric Oxide-Independent Mechanism
PLoS ONE
Frann Antignano and Melisa Hamilton and Scott Patterson and Victor Ho and Carla Cohen and Megan K. Levings and Gerald Krystal
DOI: 10.1371/journal.pone.0021893
07/2011

Environmental influences on T regulatory cells in inflammatory bowel disease
Seminars in Immunology
Gijs Hardenberg and Theodore S. Steiner and Megan K. Levings
DOI: 10.1016/j.smim.2011.01.012
04/2011

Human CD4+FOXP3+ regulatory T cells produce CXCL8 and recruit neutrophils
European Journal of Immunology
Megan E. Himmel and Sarah Q. Crome and Sabine Ivison and Ciriaco Piccirillo and Theodore S. Steiner and Megan K. Levings
DOI: 10.1002/eji.201040459
01/2011

The Stress signal extracellular ATP modulates antiflagellin immune responses in intestinal epithelial cells
Inflammatory Bowel Diseases
Sabine M. Ivison and Megan E. Himmel and Matt Mayer and Yu Yao and Arnawaz Kifayet and Megan K. Levings and Theodore S. Steiner
DOI: 10.1002/ibd.21428
01/2011

Role of epithelial integrin-linked kinase in promoting intestinal inflammation: effects on CCL2, fibronectin and the T cell repertoire
BMC Immunology
Kiran Assi and Scott Patterson and Shoukat Dedhar and David Owen and Megan Levings and Baljinder Salh
DOI: 10.1186/1471-2172-12-42
2011

Suppression assays with human T regulatory cells: A technical guide
European Journal of Immunology
Alicia N. McMurchy and Megan K. Levings
DOI: 10.1002/eji.201141651
2011

T Regulatory Cells and Cancer Immunotherapy
Experimental and Applied Immunotherapy
Adele Y. Wang and Megan K. Levings
DOI: 10.1007/978-1-60761-980-2_10
11/2010

Natural killer T cells constitutively expressing the interleukin-2 receptor a chain early in life are primed to respond to lower antigenic stimulation
Immunology
Mihoko Ladd and Ashish Sharma and Qing Huang and Adele Y. Wang and Lixin Xu and Indira Genowati and Megan K. Levings and Pascal M. Lavoie
DOI: 10.1111/j.1365-2567.2010.03304.x
09/2010

Leishmania Exosomes Modulate Innate and Adaptive Immune Responses through Effects on Monocytes and Dendritic Cells
The Journal of Immunology
J. M. Silverman and J. Clos and E. Horakova and A. Y. Wang and M. Wiesgigl and I. Kelly and M. A. Lynn and W. R. McMaster and L. J. Foster and M. K. Levings and N. E. Reiner
DOI: 10.4049/jimmunol.1000541
09/2010

Deconvolution and chromatic aberration corrections in quantifying colocalization of a transcription factor in three-dimensional cellular space
Micron
Thomas Abraham and Sarah E. Allan and Megan K. Levings
DOI: 10.1016/j.micron.2010.03.005
08/2010

Inflammatory Effects of Ex Vivo Human Th17 Cells Are Suppressed by Regulatory T Cells
The Journal of Immunology
S. Q. Crome and B. Clive and A. Y. Wang and C. Y. Kang and V. Chow and J. Yu and A. Lai and A. Ghahary and R. Broady and M. K. Levings
DOI: 10.4049/jimmunol.1000557
08/2010

CD161 is a marker of all human IL-17-producing T-cell subsets and is induced by RORC
European Journal of Immunology
Laura Maggi and Veronica Santarlasci and Manuela Capone and Anna Peired and Francesca Frosali and Sarah Q. Crome and Valentina Querci and Massimiliano Fambrini and Francesco Liotta and Megan K. Levings and Enrico Maggi and Lorenzo Cosmi and Sergio Romagnani and Francesco Annunziato
DOI: 10.1002/eji.200940257
05/2010

TLR5 is not required for flagellin-mediated exacerbation of DSS colitis
Inflammatory Bowel Diseases
Sabine M. Ivison and Megan E. Himmel and Gijs Hardenberg and Paige A.J. Wark and Arnawaz Kifayet and Megan K. Levings and Theodore S. Steiner
DOI: 10.1002/ibd.21097
03/2010

PHLPP Regulates the Development, Function and Molecular Signaling Pathways of T Regulatory Cells
Clinical Immunology
Scott Patterson and Rosa Garcia and Audrey O'Neill and Tianyan Gao and Alexandra Newton and Megan Levings
DOI: 10.1016/j.clim.2010.03.025
01/2010

Constitutive Expression of CD25 on the Surface of Neonatal (invariant) Natural Killer T Cells Primes Them to Proliferate with Lower Antigenic Stimulation
Clinical Immunology
Ashish Sharma and Mihoko Whalen and Qing Huang and Adele Wang and Lixin Xu and Indira Genowati and Meghan Levings and Pascal Lavoie
DOI: 10.1016/j.clim.2010.03.151
01/2010

The Inflammatory Effects of ex vivo Human T Helper 17 Cells are Suppressed by T Regulatory Cells
Clinical Immunology
Sarah Crome and Breanna Clive and Adele Wang and Christine Kang and Vickie Chow and Jessie Yiu and Raewyn Broady and Megan Levings
DOI: 10.1016/j.clim.2010.03.361
01/2010

Increased Fraction of IL-17 Secreting Cells in Type 1 Diabetes
Clinical Immunology
Ashish Marwaha and Megan Levings and Sarah Crome and Constadina Panagiotopoulos and John Priatel and Rusung Tan
DOI: 10.1016/j.clim.2010.03.398
01/2010

Adenoviral-transduced dendritic cells are susceptible to suppression by T regulatory cells and promote interleukin 17 production
Cancer Immunology, Immunotherapy
Adele Y. Wang and Sarah Q. Crome and Kristina M. Jenkins and Jeffrey A. Medin and Jonathan L. Bramson and Megan K. Levings
DOI: 10.1007/s00262-010-0948-4
2010

Point mutants of forkhead box P3 that cause immune dysregulation, polyendocrinopathy, enteropathy, X-linked have diverse abilities to reprogram T cells into regulatory T cells
Journal of Allergy and Clinical Immunology
Alicia N. McMurchy and Jana Gillies and Sarah E. Allan and Laura Passerini and Eleonora Gambineri and Maria Grazia Roncarolo and Rosa Bacchetta and Megan K. Levings
DOI: 10.1016/j.jaci.2010.09.001
2010

The role of retinoic acid‐related orphan receptor variant 2 and IL‐17 in the development and function of human CD4+T cells
European Journal of Immunology
Sarah Q. Crome and Adele Y. Wang and Christine Y. Kang and Megan K. Levings
DOI: 10.1002/eji.200838908
06/2009

SHIP Regulates the Reciprocal Development of T Regulatory and Th17 Cells
The Journal of Immunology
N. R. Locke and S. J. Patterson and M. J. Hamilton and L. M. Sly and G. Krystal and M. K. Levings
DOI: 10.4049/jimmunol.0803749
06/2009

Quantifying colocalization of a conditionally active transcription factor FOXP3 in three-dimensional cellular space
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XVI
Thomas Abraham and Sarah E. Allan and Megan K. Levings
DOI: 10.1117/12.808049
02/2009

Molecular Regulation ofCellular Immunity by FOXP3
Forkhead Transcription Factors
Alicia N. McMurchy and Sara Di Nunzio and Maria Grazia Roncarolo and Rosa Bacchetta and Megan K. Levings
DOI: 10.1007/978-1-4419-1599-3_3
2009

Graft versus host disease: suppression by statins
Nature Medicine
Raewyn Broady and Megan K Levings
DOI: 10.1038/nm1108-1155
11/2008

Inducible reprogramming of human T cells into Treg cells by a conditionally active form of FOXP3
European Journal of Immunology
Sarah E. Allan and George X. Song-Zhao and Thomas Abraham and Alicia N. McMurchy and Megan K. Levings
DOI: 10.1002/eji.200838373
11/2008

The role of T-regulatory cells and Toll-like receptors in the pathogenesis of human inflammatory bowel disease
Immunology
Megan E. Himmel and Gijs Hardenberg and Ciriaco A. Piccirillo and Theodore S. Steiner and Megan K. Levings
DOI: 10.1111/j.1365-2567.2008.02939.x
10/2008

CD4+T-regulatory cells: toward therapy for human diseases
Immunological Reviews
Sarah E. Allan and Raewyn Broady and Silvia Gregori and Megan E. Himmel and Natasha Locke and Maria Grazia Roncarolo and Rosa Bacchetta and Megan K. Levings
DOI: 10.1111/j.1600-065x.2008.00634.x
06/2008

STAT5-signaling cytokines regulate the expression of FOXP3 in CD4+CD25+ regulatory T cells and CD4+CD25- effector T cells
International Immunology
Laura Passerini and Sarah E. Allan and Manuela Battaglia and Sara Di Nunzio and Alicia N. Alstad and Megan K. Levings and Maria G. Roncarolo and Rosa Bacchetta
DOI: 10.1093/intimm/dxn002
02/2008

Expression of RORC2 Controls the Development of Human Th17 Cells
Clinical Immunology
Sarah Crome and Adele Wang and Megan Levings
DOI: 10.1016/j.clim.2008.03.043
01/2008

Molecular and Functional Studies in T Cells from Healthy Carriers of FOXP3 Mutations
Clinical Immunology
Rosa Bacchetta and Sara Di Nunzio and Lucia Beatrice Perroni and Laura Passerini and Sarah Allan and Alicia Alstad and Anne Junker and Megan Levings and Maria Grazia Roncarolo
DOI: 10.1016/j.clim.2008.03.187
01/2008

Generation of Potent and Stable Human CD4+ T Regulatory Cells by Activation-independent Expression of FOXP3
Molecular Therapy
Sarah E Allan and Alicia N Alstad and Natacha Merindol and Natasha K Crellin and Mario Amendola and Rosa Bacchetta and Luigi Naldini and Maria Grazia Roncarolo and Hugo Soudeyns and Megan K Levings
DOI: 10.1038/sj.mt.6300341
01/2008

Molecular Signalling in T Regulatory Cells
Regulatory T Cells and Clinical Application
Natasha R. Locke and Natasha K. Crellin and Megan K. Levings
DOI: 10.1007/978-0-387-77909-6_8
2008

Pro-tolerogenic effects of photodynamic therapy with TH9402 on dendritic cells
Journal of Clinical Apheresis
Raewyn Broady and Jie Yu and Megan K. Levings
DOI: 10.1002/jca.20162
2008

27 Effects of IL-17 On Effector and Regultory T Cell Subsets
Cytokine
Sarah Q. Crome and Adele Wang and Megan K. Levings
DOI: 10.1016/j.cyto.2007.07.032
07/2007

Flow cytometry-based methods for studying signaling in human CD4+CD25+FOXP3+ T regulatory cells
Journal of Immunological Methods
Natasha K. Crellin and Rosa V. Garcia and Megan K. Levings
DOI: 10.1016/j.jim.2007.05.008
07/2007

Altered activation of AKT is required for the suppressive function of human CD4+CD25+ T regulatory cells
Blood
N. K. Crellin and R. V. Garcia and M. K. Levings
DOI: 10.1182/blood-2006-07-035279
03/2007

Activation-induced FOXP3 in human T effector cells does not suppress proliferation or cytokine production
International Immunology
S. E. Allan and S. Q. Crome and N. K. Crellin and L. Passerini and T. S. Steiner and R. Bacchetta and M. G. Roncarolo and M. K. Levings
DOI: 10.1093/intimm/dxm014
02/2007

Isolation, Expansion, and Characterization of Human Natural and Adaptive Regulatory T Cells
Immunological Tolerance
Silvia Gregori and Rosa Bacchetta and Laura Passerini and Megan K. Levings and Maria Grazia Roncarolo
DOI: 10.1007/978-1-59745-395-0_6
2007

Gliadin-Specific Type 1 Regulatory T Cells from the Intestinal Mucosa of Treated Celiac Patients Inhibit Pathogenic T Cells
The Journal of Immunology
C. Gianfrani and M. K. Levings and C. Sartirana and G. Mazzarella and G. Barba and D. Zanzi and A. Camarca and G. Iaquinto and N. Giardullo and S. Auricchio and R. Troncone and M.-G. Roncarolo
DOI: 10.4049/jimmunol.177.6.4178
09/2006

Interleukin-10-secreting type 1 regulatory T cells in rodents and humans
Immunological Reviews
Maria Grazia Roncarolo and Silvia Gregori and Manuela Battaglia and Rosa Bacchetta and Katharina Fleischhauer and Megan K. Levings
DOI: 10.1111/j.0105-2896.2006.00420.x
08/2006

Su.98. Defining a Role for FOXP3 in Human Cd4+ T-Cells
Clinical Immunology
Sarah Allan and Laura Passerini and Rosa Bacchetta and Mario Amendola and Luigi Naldini and Maria Grazia Roncarolo and Megan Levings
DOI: 10.1016/j.clim.2006.04.525
01/2006

Human CD4+CD25+ T Regulatory Cells Have Altered PKB Signalling Which Correlates with Suppressive Function
Clinical Immunology
Natasha Crellin and Rosa Garcia and Megan Levings
DOI: 10.1016/j.clim.2006.04.009
01/2006

Foxp3 Mutations in Children: Different Degrees of Impairment in Regulatory and Effector T-Cell Functions in Patients with Distinct Genotypes and Phenotypes
Clinical Immunology
Rosa Bacchetta and Laura Passerini and Eleonora Gambineri and Sarah Allan and Lucia Perroni and Susan Matthes-Martin and Anita Lawitschka and Steven Ziegler and Megan Levings and Maria Grazia Roncarolo
DOI: 10.1016/j.clim.2006.04.196
01/2006

Functional Dynamics of Naturally Occurring Regulatory T Cells in Health and Autoimmunity
Advances in Immunology
Megan K. Levings and Sarah Allan and Eva d'Hennezel and Ciriaco A. Piccirillo
DOI: 10.1016/s0065-2776(06)92003-3
2006

Human CD4+ T Cells Express TLR5 and Its Ligand Flagellin Enhances the Suppressive Capacity and Expression of FOXP3 in CD4+CD25+ T Regulatory Cells
The Journal of Immunology
N. K. Crellin and R. V. Garcia and O. Hadisfar and S. E. Allan and T. S. Steiner and M. K. Levings
DOI: 10.4049/jimmunol.175.12.8051
2005

Differentiation of Tr1 cells by immature dendritic cells requires IL-10 but not CD25+CD4+ Tr cells
Blood
M. K. Levings
DOI: 10.1182/blood-2004-03-1211
09/2004

Can we throw the master-switch in autoimmune disease?
Clinical Genetics
Paul C Orban and Megan K Levings
DOI: 10.1034/j.1399-0004.2003.t01-2-00121.x
06/2003

Discoveries in sphingolipid metabolism, spinocerebellar ataxia and autoimmune disease
Clinical Genetics
Liam R Brunham and Edmond YW Chan and Paul C Orban and Megan K Levings
DOI: 10.1034/j.1399-0004.2003.00121.x
06/2003

Human CD25+CD4+T Suppressor Cell Clones Produce Transforming Growth Factor ß, but not Interleukin 10, and Are Distinct from Type 1 T Regulatory Cells
The Journal of Experimental Medicine
Megan K. Levings and Romina Sangregorio and Claudia Sartirana and Anna Lisa Moschin and Manuela Battaglia and Paul C. Orban and Maria-Grazia Roncarolo
DOI: 10.1084/jem.20021139
11/2002

Growth and expansion of human T regulatory type 1 cells are independent from TCR activation but require exogenous cytokines
European Journal of Immunology
Rosa Bacchetta and Claudia Sartirana and Megan K. Levings and Claudio Bordignon and Satwant Narula and Maria-Grazia Roncarolo
DOI: 10.1002/1521-4141(200208)32:83.0.co;2-2
08/2002

The Role of IL-10 and TGF-ß in the Differentiation and Effector Function of T Regulatory Cells
International Archives of Allergy and Immunology
Megan K. Levings and Rosa Bacchetta and Ute Schulz and Maria Grazia Roncarolo
DOI: 10.1159/000067596
2002

Type 1 T regulatory cells
Immunological Reviews
Maria Grazia Roncarolo and Rosa Bacchetta and Claudio Bordignon and Satwant Narula and Megan K. Levings
DOI: 10.1034/j.1600-065x.2001.1820105.x
08/2001

Human Cd25+Cd4+T Regulatory Cells Suppress Naive and Memory T Cell Proliferation and Can Be Expanded in Vitro without Loss of Function
The Journal of Experimental Medicine
Megan K. Levings and Romina Sangregorio and Maria-Grazia Roncarolo
DOI: 10.1084/jem.193.11.1295
06/2001

IFN-  and IL-10 Induce the Differentiation of Human Type 1 T Regulatory Cells
The Journal of Immunology
M. K. Levings and R. Sangregorio and F. Galbiati and S. Squadrone and R. de Waal Malefyt and M.-G. Roncarolo
DOI: 10.4049/jimmunol.166.9.5530
05/2001

T-regulatory 1 cells: A novel subset of CD4+ T cells with immunoregulatory properties
Journal of Allergy and Clinical Immunology
Megan K Levings and Maria-Grazia Roncarolo
DOI: 10.1067/mai.2000.106635
07/2000

The role of different subsets of T regulatory cells in controlling autoimmunity
Current Opinion in Immunology
Maria-Grazia Roncarolo and Megan K Levings
DOI: 10.1016/s0952-7915(00)00162-x
2000

Insulin Receptor Substrate-2 Is the Major 170-kDa Protein Phosphorylated on Tyrosine in Response to Cytokines in Murine Lymphohemopoietic Cells
Journal of Biological Chemistry
Melanie J. Welham and Heather Bone and Megan Levings and Leslie Learmonth and Ling-Mei Wang and Kevin B. Leslie and Jacalyn H. Pierce and John W. Schrader
DOI: 10.1074/jbc.272.2.1377
01/1997

Isolation, Expansion, and Characterization of Human Natural and Adaptive Regulatory T Cells
Immunological Tolerance
Silvia Gregori and Rosa Bacchetta and Laura Passerini and Megan K. Levings and Maria Grazia Roncarolo
DOI: 10.1385/1-59745-395-1:83

Type 1 T Regulatory Cells and Their Relationship with CD4+CD25+ T Regulatory Cells
Generation and Effector Functions of Regulatory Lymphocytes
Maria Grazia Roncarolo and Silvia Gregori and Megan Levings
DOI: 10.1002/0470871628.ch9

Phenotypic and Functional Differences Between Human CD4+CD25+ and Type 1 Regulatory T Cells
CD4+CD25+ Regulatory T Cells: Origin, Function and Therapeutic Potential
M. K. Levings and M. G. Roncarolo
DOI: 10.1007/3-540-27702-1_14

Research

Current Projects
Studying the interaction between Tregs and IL-17-producing cells
Developing biomarkers to track the function of Tregs
Studying the unique cell-signalling pathways in human and mouse Tregs

Grants

Genome Canada and CIHR: Applied Research Project Competition in Genomics and Personalized Health (2013)

Canadian Cancer Society Research Institute: "Generation of antigen specific T regulatory cells to treat GVHD post allogeneic stem cell transplant" (2013)

CIHR and Genome BC: Canadian National Transplant Research Program (2013)

Honours & Awards

2012 Genome Canada-CIHR Competition in Personalized Health

2016 UBC Killam Research Prize in Applied Science & Sciences

2003-2013 CRC in Transplantation Tier II; Canada Research Chairs

Research Group Members

Dominic Boardman
Daphne Brockington, Clinical Trial Manager
Sunny Chen
Laura Cook
Nicholas Dawson
Vivian Fung, Lab Technician
Rosa Garcia
Jana Gillies
Miika Hakkinen, Co-op Student
Romy Hoeppli
Jessica Huang
Mitsu Komba, Lab Tech
Avery Lam, Doctoral Student
Caroline Lamarche, Post-doctoral fellow
Katie MacDonald, Doctoral Student
Majid Mojibian, Research & Development Manager
Uyen Nguyen, Masters Student
Anne Pesenacker
Kyle Reid, Student
Isaac Rosado Sanchez, Postdoctoral Fellow
Antoine Sicard, Postdoctoral Fellow
Madeleine Speck
Kaija Strautins, Research Coordinator
Kirsten Ward Harstonge, Postdoctoral Fellow
May Wong
Dan Wu