Consultation and study design
- Consultation service for the design and implementation of human and animal gut microbiome studies – including assistance with ethics and grant applications
- Sample preparation and quality control tests
- Optimal sample collection - fecal collection kits
- Sample size calculations
Storage of clinical specimens
- Fecal sample, mucosal wash, swab and biopsy storage (-80oC)
- Maintenance of a sample inventory
- Sample processing/aliquoting of liquids, body fluids and fecal specimens
- Microbial cultivation and freezing of bacterial isolates
Nucleic acid isolation and quantification
- Genomic DNA extraction
- Qubit and nanodrop quantification
Next generation sequencing
- 16S rRNA sequencing
- Shotgun metagenomic sequencing
- 18S rRNA sequencing
- ITS sequencing
Anaerobic culturing
- Bacterial culturing under strict anaerobic conditions
Metabolomics
- Short-chain fatty acids, amino acids and bile acids
Targeted Microbiome enumeration
- QX200 automated droplet digital PCR
In vitro and in vivo assays related to microbiome investigation
Data Analysis and Bioinformatics
- Standardized basic 16S rRNA sequencing analysis
- Advanced bioinformatics capabilities available by request
Instruments available in the microbiome core facility
A35 Don Whitley Anaerobic chamber
BioRad QX200 automated droplet digital PCR
Pre-PCR hood
Lyophilizer
nanodrop
qubit
Service requests and fees
Contact us for service requests and click on the button below for fee information.